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The map label for this gene is waaA [H]

Identifier: 218531146

GI number: 218531146

Start: 3375781

End: 3377085

Strand: Reverse

Name: waaA [H]

Synonym: Mchl_3196

Alternate gene names: 218531146

Gene position: 3377085-3375781 (Counterclockwise)

Preceding gene: 218531147

Following gene: 218531145

Centisome position: 58.45

GC content: 71.95

Gene sequence:

>1305_bases
GTGAGGGTCCCGAAGCTTCCCGTCGCCCTGCGCGCCTACCGCTACGGACTCTATCTCGGCGAACCCGCCGTGGCCGGGCT
GCTGGCGTGGCGCTCGCGCCGGGGCAAGGAGGATCCGGTCCGGCTGTCCGAGCGTCGCGGCCTGCCCGGCCGGGCGCGGC
CGGTGGGCCATCTCGTCTGGATGCACGGGGCGAGCATCGGCGAGGCGCTTTCCCTCGTCGGGTTGATCGAGGGCATGATC
GCCCGCGGCTGCTCGGTTCTCGTCACCACCGGCACGCGCAGCGCCGCCGACCTGCTGAGCAAGCGCCTGCCCCCCGGCGC
CGTGCATCAGTACATGCCGCTCGACGCGCCGCGCTGGATCGAGCGCTTCCTCGCGCATTGGCAGCCCGATCTCGCTGTCG
TCGCCGAATCCGAGATCTGGCCCAACACCATCGTGTCGCTGCACCGCCGGGGCATCCCGCTGGTGCTCGTCAACGGCCGG
ATGTCGGAGCGTTCGTTCAAGGCCTGGACGCGCTCGCCCGACACCGCTCGGGCGCTGCTGGCGCGGATCGCGGTCTGCCT
CGTCCAGACCCGCGAGGATGGCGAGCGCTTCGCCCGGCTCGGTGCGCCGCGCATCAACGTCGTCGGCAATCTCAAATACG
ATTCGGCCGTGCCACCCGCCGATTCGCAGCAACTCGCCTATCTCGGCGACATGATCGGGGACCGGCCCGTCTGGGTCGCG
GCCAGCACCCATGCCGGCGAGGACGAGGTCGTCGCCCGTGTCCATGCCGGCCTGAAGGCGCGTTTCCCGCGCCTGCTGAC
GGTGATCGTGCCGCGCCATCCGCGCCGGGGCGAGGAAGCGGCGCGGATTGCCACCGCCGCCGGCCTGCGGGTGAGCCGGC
GCGCCAAGGGCGGGCGTCCGCTGCCCTCGATCGACCTCTACGTCGCCGACACGATCGGCGAACTCGGTCTGTTCTACCGG
CTCTGCCCCCTGGTCTTCCTCGGTGGCTCGCTGGTGCCGCATGGCGGCCAGAACCCGATCGAGCCGGTGCGCCTGGACAG
CGCGATCCTGCATGGGCCGCATGTCCACAACTTCCACGAACCCTACGGCGCGCTCGACACCAGGGGCGGCGCGCGTCGGG
TCGCGGACGAGGCGGAGCTTCAGAAGGCCGTCGGCGAACTGGTCGCCGACCCGCGTGCGCTCGCGGCGATGTGGGCCAAG
GGCCAGGCCGCGCTCCTGCCCTTCGAGGGCGCGGTTGGGCGCACCTTCGCGGTGCTCGAACCCTACGTCGCGCAGATGAA
GCTCTCCGCGCGCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
AAGACGAGCTGAACCGCGTCCACGACCGGGCCTACACCCTCGTCGGCCGGCCGGACCGGGTCGGCGCCGTGTCCGCGCCC
GCATCGCAGGGCAGCCCGGC

Downstream 100 bases:

>100_bases
GGCCGCGATGCGTCCGCCCGGCTTCTGGACCCGGCCGCCCACGCATCCGCTCGCGCGGCTGCTGGCCCCCGTCGGCCGGG
TCTATGGCGGCCTGACCGCC

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRVPKLPVALRAYRYGLYLGEPAVAGLLAWRSRRGKEDPVRLSERRGLPGRARPVGHLVWMHGASIGEALSLVGLIEGMI
ARGCSVLVTTGTRSAADLLSKRLPPGAVHQYMPLDAPRWIERFLAHWQPDLAVVAESEIWPNTIVSLHRRGIPLVLVNGR
MSERSFKAWTRSPDTARALLARIAVCLVQTREDGERFARLGAPRINVVGNLKYDSAVPPADSQQLAYLGDMIGDRPVWVA
ASTHAGEDEVVARVHAGLKARFPRLLTVIVPRHPRRGEEAARIATAAGLRVSRRAKGGRPLPSIDLYVADTIGELGLFYR
LCPLVFLGGSLVPHGGQNPIEPVRLDSAILHGPHVHNFHEPYGALDTRGGARRVADEAELQKAVGELVADPRALAAMWAK
GQAALLPFEGAVGRTFAVLEPYVAQMKLSARERD

Sequences:

>Translated_434_residues
MRVPKLPVALRAYRYGLYLGEPAVAGLLAWRSRRGKEDPVRLSERRGLPGRARPVGHLVWMHGASIGEALSLVGLIEGMI
ARGCSVLVTTGTRSAADLLSKRLPPGAVHQYMPLDAPRWIERFLAHWQPDLAVVAESEIWPNTIVSLHRRGIPLVLVNGR
MSERSFKAWTRSPDTARALLARIAVCLVQTREDGERFARLGAPRINVVGNLKYDSAVPPADSQQLAYLGDMIGDRPVWVA
ASTHAGEDEVVARVHAGLKARFPRLLTVIVPRHPRRGEEAARIATAAGLRVSRRAKGGRPLPSIDLYVADTIGELGLFYR
LCPLVFLGGSLVPHGGQNPIEPVRLDSAILHGPHVHNFHEPYGALDTRGGARRVADEAELQKAVGELVADPRALAAMWAK
GQAALLPFEGAVGRTFAVLEPYVAQMKLSARERD
>Mature_434_residues
MRVPKLPVALRAYRYGLYLGEPAVAGLLAWRSRRGKEDPVRLSERRGLPGRARPVGHLVWMHGASIGEALSLVGLIEGMI
ARGCSVLVTTGTRSAADLLSKRLPPGAVHQYMPLDAPRWIERFLAHWQPDLAVVAESEIWPNTIVSLHRRGIPLVLVNGR
MSERSFKAWTRSPDTARALLARIAVCLVQTREDGERFARLGAPRINVVGNLKYDSAVPPADSQQLAYLGDMIGDRPVWVA
ASTHAGEDEVVARVHAGLKARFPRLLTVIVPRHPRRGEEAARIATAAGLRVSRRAKGGRPLPSIDLYVADTIGELGLFYR
LCPLVFLGGSLVPHGGQNPIEPVRLDSAILHGPHVHNFHEPYGALDTRGGARRVADEAELQKAVGELVADPRALAAMWAK
GQAALLPFEGAVGRTFAVLEPYVAQMKLSARERD

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=417, Percent_Identity=35.9712230215827, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47324; Mature: 47324

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPKLPVALRAYRYGLYLGEPAVAGLLAWRSRRGKEDPVRLSERRGLPGRARPVGHLVW
CCCCCCCHHHHHHHHHEEECCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHEEEE
MHGASIGEALSLVGLIEGMIARGCSVLVTTGTRSAADLLSKRLPPGAVHQYMPLDAPRWI
ECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHCCCCCCHHHH
ERFLAHWQPDLAVVAESEIWPNTIVSLHRRGIPLVLVNGRMSERSFKAWTRSPDTARALL
HHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHHH
ARIAVCLVQTREDGERFARLGAPRINVVGNLKYDSAVPPADSQQLAYLGDMIGDRPVWVA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
ASTHAGEDEVVARVHAGLKARFPRLLTVIVPRHPRRGEEAARIATAAGLRVSRRAKGGRP
ECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
LPSIDLYVADTIGELGLFYRLCPLVFLGGSLVPHGGQNPIEPVRLDSAILHGPHVHNFHE
CCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHCCCCCCCCCC
PYGALDTRGGARRVADEAELQKAVGELVADPRALAAMWAKGQAALLPFEGAVGRTFAVLE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
PYVAQMKLSARERD
HHHHHHHHHHCCCC
>Mature Secondary Structure
MRVPKLPVALRAYRYGLYLGEPAVAGLLAWRSRRGKEDPVRLSERRGLPGRARPVGHLVW
CCCCCCCHHHHHHHHHEEECCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHEEEE
MHGASIGEALSLVGLIEGMIARGCSVLVTTGTRSAADLLSKRLPPGAVHQYMPLDAPRWI
ECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHCCCCCCHHHH
ERFLAHWQPDLAVVAESEIWPNTIVSLHRRGIPLVLVNGRMSERSFKAWTRSPDTARALL
HHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHHH
ARIAVCLVQTREDGERFARLGAPRINVVGNLKYDSAVPPADSQQLAYLGDMIGDRPVWVA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
ASTHAGEDEVVARVHAGLKARFPRLLTVIVPRHPRRGEEAARIATAAGLRVSRRAKGGRP
ECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
LPSIDLYVADTIGELGLFYRLCPLVFLGGSLVPHGGQNPIEPVRLDSAILHGPHVHNFHE
CCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHCCCCCCCCCC
PYGALDTRGGARRVADEAELQKAVGELVADPRALAAMWAKGQAALLPFEGAVGRTFAVLE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
PYVAQMKLSARERD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]