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The map label for this gene is ada [H]

Identifier: 218531089

GI number: 218531089

Start: 3315452

End: 3316378

Strand: Reverse

Name: ada [H]

Synonym: Mchl_3139

Alternate gene names: 218531089

Gene position: 3316378-3315452 (Counterclockwise)

Preceding gene: 218531090

Following gene: 218531088

Centisome position: 57.4

GC content: 71.31

Gene sequence:

>927_bases
ATGACGCGCACCCCTTCGCCTGCGACCCGAGAGGACGGGGCCTGTTTTACGCTGGCCTGCCGCCAGCCCTACGATTGGGC
GCACCTCGAACGCTTCTTCGCCGATCATGCGAGCCCCGGCGTCGAAACCGTGACGCCCGGCCGCTATGCCCGCACCTTCG
CCCTCGACGGGCATCGCGGCTCCGTCTCCGTGGCGGCTGAGGCGAATGTTCTGTCGGTCCGTATCCGCGGCCTCGGCTCG
GATGACGACCACGAAAACATCCTTGCGCGGGTGCGGGCGATGTTCGATCTCGACGCCGACCCGCGGGCGATCGCTGCCGG
ACTCGGCCGCGATCCGTTCACCGCTGGCCTCGTGGGGCGCCGGCCGGGTTTGCGGATGCCGGGCGCCTTCGACGGGTTCG
AACTGGCGGTCCGGGCGATCCTCGGCCAGCAGGTCTCGGTCGCGGCCGCCACGCGTCTCGCCGGCCGACTCGTCGCGGGT
TTCGGTACGCCCCTCGACGGGGAGACGTGCGGGAACGAGCCGGGTCTCACGCACCTGTTTCCGACGCCGAAGCAGTTGGT
GGACGCCGAAATCTCGCTCGTGCTCAACATGCCCCGCGCCCGCGGCCGGGCGATCCAGGGGCTTGCCGCGGCAATGCTCG
CCATGCCGGATCTGTTCGCGCCCGGCGGCGAACTCGACGCGACGGTTGCCCGGCTGAAGGCCCTGCCCGGCATCGGGGAC
TGGACCGCGCATTACGTCGCCATGCGCGCACTGGCGCAAGCCGACGCCTTTCCGGCGGGGGATGTCGGGCTGATGCGTGC
CCTCGATGCCGGCGACGGGCGGCCGAGCCGGCTGGCACTTCTCGACCGTGCGGCGGCTTGGCGGCCCTGGCGAGCCTATG
CCGCGATCCATCTCTGGGCCGAGGATGCCGCGCGGAGGATGCCATGA

Upstream 100 bases:

>100_bases
GTGGGCGATACCGCGGAGTTCGAGAGCACCGAGCCGGTTGCGCCGGGCTGACGCCGGCCCAACAGTGGGGCCGAATTCCT
GCCACGCTCCCCACTGATCC

Downstream 100 bases:

>100_bases
CGATCCGTGATCCCGCCGCTGCCGACGAGGCCGGATGGCACCGGCTCTGGGCCGGCTACCTCGCCTTCTACGGTGAGACT
CTGCCGGAGGCGGTCACCGC

Product: AlkA domain protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTRTPSPATREDGACFTLACRQPYDWAHLERFFADHASPGVETVTPGRYARTFALDGHRGSVSVAAEANVLSVRIRGLGS
DDDHENILARVRAMFDLDADPRAIAAGLGRDPFTAGLVGRRPGLRMPGAFDGFELAVRAILGQQVSVAAATRLAGRLVAG
FGTPLDGETCGNEPGLTHLFPTPKQLVDAEISLVLNMPRARGRAIQGLAAAMLAMPDLFAPGGELDATVARLKALPGIGD
WTAHYVAMRALAQADAFPAGDVGLMRALDAGDGRPSRLALLDRAAAWRPWRAYAAIHLWAEDAARRMP

Sequences:

>Translated_308_residues
MTRTPSPATREDGACFTLACRQPYDWAHLERFFADHASPGVETVTPGRYARTFALDGHRGSVSVAAEANVLSVRIRGLGS
DDDHENILARVRAMFDLDADPRAIAAGLGRDPFTAGLVGRRPGLRMPGAFDGFELAVRAILGQQVSVAAATRLAGRLVAG
FGTPLDGETCGNEPGLTHLFPTPKQLVDAEISLVLNMPRARGRAIQGLAAAMLAMPDLFAPGGELDATVARLKALPGIGD
WTAHYVAMRALAQADAFPAGDVGLMRALDAGDGRPSRLALLDRAAAWRPWRAYAAIHLWAEDAARRMP
>Mature_307_residues
TRTPSPATREDGACFTLACRQPYDWAHLERFFADHASPGVETVTPGRYARTFALDGHRGSVSVAAEANVLSVRIRGLGSD
DDHENILARVRAMFDLDADPRAIAAGLGRDPFTAGLVGRRPGLRMPGAFDGFELAVRAILGQQVSVAAATRLAGRLVAGF
GTPLDGETCGNEPGLTHLFPTPKQLVDAEISLVLNMPRARGRAIQGLAAAMLAMPDLFAPGGELDATVARLKALPGIGDW
TAHYVAMRALAQADAFPAGDVGLMRALDAGDGRPSRLALLDRAAAWRPWRAYAAIHLWAEDAARRMP

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=290, Percent_Identity=34.4827586206897, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32779; Mature: 32648

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTPSPATREDGACFTLACRQPYDWAHLERFFADHASPGVETVTPGRYARTFALDGHRG
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
SVSVAAEANVLSVRIRGLGSDDDHENILARVRAMFDLDADPRAIAAGLGRDPFTAGLVGR
CEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHCCCCC
RPGLRMPGAFDGFELAVRAILGQQVSVAAATRLAGRLVAGFGTPLDGETCGNEPGLTHLF
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PTPKQLVDAEISLVLNMPRARGRAIQGLAAAMLAMPDLFAPGGELDATVARLKALPGIGD
CCHHHHHHHHEEEEEECCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCH
WTAHYVAMRALAQADAFPAGDVGLMRALDAGDGRPSRLALLDRAAAWRPWRAYAAIHLWA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHEEEEEEH
EDAARRMP
HHHHHCCC
>Mature Secondary Structure 
TRTPSPATREDGACFTLACRQPYDWAHLERFFADHASPGVETVTPGRYARTFALDGHRG
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
SVSVAAEANVLSVRIRGLGSDDDHENILARVRAMFDLDADPRAIAAGLGRDPFTAGLVGR
CEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHCCCCC
RPGLRMPGAFDGFELAVRAILGQQVSVAAATRLAGRLVAGFGTPLDGETCGNEPGLTHLF
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PTPKQLVDAEISLVLNMPRARGRAIQGLAAAMLAMPDLFAPGGELDATVARLKALPGIGD
CCHHHHHHHHEEEEEECCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCH
WTAHYVAMRALAQADAFPAGDVGLMRALDAGDGRPSRLALLDRAAAWRPWRAYAAIHLWA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHEEEEEEH
EDAARRMP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]