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The map label for this gene is ybiS [H]

Identifier: 218531069

GI number: 218531069

Start: 3301593

End: 3302270

Strand: Reverse

Name: ybiS [H]

Synonym: Mchl_3119

Alternate gene names: 218531069

Gene position: 3302270-3301593 (Counterclockwise)

Preceding gene: 218531072

Following gene: 218531068

Centisome position: 57.15

GC content: 68.88

Gene sequence:

>678_bases
ATGGCAGCCCCCCGAACCGCCGATAGGACGGCGCGCAGCACCCGCCGGGTCGTGCTCGCCGCGCTCTGCCTCATTCCCCT
GGCCGCCTGCCAATCCTCGAAGCCGGGCCCGATGGCCGGCGGCGACGACGACGCGGCCTGGTACATCGGCACGATGCCCG
ACAAGCCGTTCGACGTGCCGCTGGTCGACCGGTCGCGCATCGACCCGAAGTACCGCCGTCAGGAGGTGGCCTATAGCGGT
CCCGAGGCGCCGGGCACGATCGTCGTCGATATCGACAAGCGTCATCTCGCTCTCGTGCAGGAGGGCGGCACGGCGCTCCA
ATACGGCGTCGGTGTCGGCAAGGCCGGCTTCTCCTGGAAGGGCGACGCCCGCATCGGCCGCAAGGGCGTCTGGCCGGATT
GGAGCCCGACCACGACGATGGTCTCGCTCAATCCGGGCATCGAGCGCTCGCGTAAGGGCGGCATCGACAACCCGCTCGGC
GCGCGCGCGCTCTACCTCTACCAAGGCAACCGCGACACGATGTTCCGCATCCACGGCACCAACGAACCGTGGAGCATCGG
CGAGCAGATGTCCTCGGGCTGCGTGCGCATGCTCAACGAGGACATCGTCGATCTCTTCGAGCGCGTCCCCGTCGGCGCCC
GTGTGGTGGTGAAGCGCAGCAGCAAGTATCGCGCCTGA

Upstream 100 bases:

>100_bases
ATGCGGCCTCGAAGTCACACCCGATCCGCTCAAGGGCGGGTAGTCATGGCGCATGATCTGTAACGCCAACCCGCCCCCGC
CGGCGGCGACAGGAGCCGCC

Downstream 100 bases:

>100_bases
GGACGCCCTCGCGTCGAAGAGCCGACGCGCGCATCCGGCTCGAGCCGGTCGGCCTCCTTGTGGGGCGCGGCCGCGTGCGG
GATCAGAACAGCCGACGGTA

Product: ErfK/YbiS/YcfS/YnhG family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MAAPRTADRTARSTRRVVLAALCLIPLAACQSSKPGPMAGGDDDAAWYIGTMPDKPFDVPLVDRSRIDPKYRRQEVAYSG
PEAPGTIVVDIDKRHLALVQEGGTALQYGVGVGKAGFSWKGDARIGRKGVWPDWSPTTTMVSLNPGIERSRKGGIDNPLG
ARALYLYQGNRDTMFRIHGTNEPWSIGEQMSSGCVRMLNEDIVDLFERVPVGARVVVKRSSKYRA

Sequences:

>Translated_225_residues
MAAPRTADRTARSTRRVVLAALCLIPLAACQSSKPGPMAGGDDDAAWYIGTMPDKPFDVPLVDRSRIDPKYRRQEVAYSG
PEAPGTIVVDIDKRHLALVQEGGTALQYGVGVGKAGFSWKGDARIGRKGVWPDWSPTTTMVSLNPGIERSRKGGIDNPLG
ARALYLYQGNRDTMFRIHGTNEPWSIGEQMSSGCVRMLNEDIVDLFERVPVGARVVVKRSSKYRA
>Mature_224_residues
AAPRTADRTARSTRRVVLAALCLIPLAACQSSKPGPMAGGDDDAAWYIGTMPDKPFDVPLVDRSRIDPKYRRQEVAYSGP
EAPGTIVVDIDKRHLALVQEGGTALQYGVGVGKAGFSWKGDARIGRKGVWPDWSPTTTMVSLNPGIERSRKGGIDNPLGA
RALYLYQGNRDTMFRIHGTNEPWSIGEQMSSGCVRMLNEDIVDLFERVPVGARVVVKRSSKYRA

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=138, Percent_Identity=35.5072463768116, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787968, Length=151, Percent_Identity=37.0860927152318, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788299, Length=150, Percent_Identity=32.6666666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787356, Length=146, Percent_Identity=32.1917808219178, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 24573; Mature: 24442

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPRTADRTARSTRRVVLAALCLIPLAACQSSKPGPMAGGDDDAAWYIGTMPDKPFDVP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
LVDRSRIDPKYRRQEVAYSGPEAPGTIVVDIDKRHLALVQEGGTALQYGVGVGKAGFSWK
CCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCCCCCCCCC
GDARIGRKGVWPDWSPTTTMVSLNPGIERSRKGGIDNPLGARALYLYQGNRDTMFRIHGT
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEECC
NEPWSIGEQMSSGCVRMLNEDIVDLFERVPVGARVVVKRSSKYRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
AAPRTADRTARSTRRVVLAALCLIPLAACQSSKPGPMAGGDDDAAWYIGTMPDKPFDVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
LVDRSRIDPKYRRQEVAYSGPEAPGTIVVDIDKRHLALVQEGGTALQYGVGVGKAGFSWK
CCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCCCCCCCCC
GDARIGRKGVWPDWSPTTTMVSLNPGIERSRKGGIDNPLGARALYLYQGNRDTMFRIHGT
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEECC
NEPWSIGEQMSSGCVRMLNEDIVDLFERVPVGARVVVKRSSKYRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]