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The map label for this gene is ppdK [H]

Identifier: 218531067

GI number: 218531067

Start: 3298319

End: 3300985

Strand: Reverse

Name: ppdK [H]

Synonym: Mchl_3117

Alternate gene names: 218531067

Gene position: 3300985-3298319 (Counterclockwise)

Preceding gene: 218531068

Following gene: 218531066

Centisome position: 57.13

GC content: 67.15

Gene sequence:

>2667_bases
ATGACGAAATGGGTCTATTCCTTCGGTGACGGCAAGGCCGAAGGTGAGGCAGCGATGCGCAACCTCCTCGGCGGCAAGGG
CGCGAACCTCGCCGAGATGTCCAATCTCGGCCTTCCGGTTCCCCCCGGCTTCACCATCACGACCGAGGTCTGCACCTACT
ATTACCAGCATGGCGAGACCTACCCGGCCGAGCTCACCGACGCGGTGAAGGAAGCTCTGGAGAAGGTCGGCACGCTCACC
GGCCGCCGCTTCGGCGATGCGGAAAAGCCGCTGCTCGTCTCGGTCCGCTCCGGCGCGCGGGCCTCGATGCCGGGCATGAT
GGACACGGTGCTCAATCTCGGCCTCAACGACGCCACCGTCGAGGCCCTGGCCAAGGATGCCGACGACGAGCGCTTCGCCT
ACGATTCCTACCGCCGCTTCATCACGATGTACTCGAACGTCGTGCTCGGCGTGGAGCACCACGCCTTCGAGGAGGCGCTG
GAGCACTACAAGGAGCAGAAGGGCCTCACCCTCGACACCGACCTGAAGGCCGACGACTGGAAGCACCTGATCGGCGTCTA
CAAGAAGATCGTCCGTGACGAGCACGGCTCCGAGTTCCCGCAAGGAGCGGAGGACCAGCTCTGGGGCGCGATCGGCGCGG
TGTTCGATTCCTGGATGATCCCGCGGGCCAAGAAGTACCGCGAGCTGAACCAGATTCCCGAGAGCTGGGGCACGGCGGTC
AACGTCCAGGCCATGGTGTTCGGCAACATGGGCGACACCTCGGCCACCGGCGTCGCCTTCACCCGCAACCCCTCGACCGG
CGAGAGCGCGCTCTACGGCGAGTTCCTCATCAACGCCCAGGGCGAGGATGTGGTGGCGGGTATCCGCACGCCGCAGGACA
TTACCGAGAAGGCCCGCATCGAGGCCAAGTCCGACAAGCCGTCGATGGAAAAGGCGATGCCCGAGAGCTTCGCCGAGCTG
ACGCGGATCTACGGGATCCTCGAGAAGCACTACCGCGACATGCAGGACATGGAGTTCACCATTGAGCGGGGTAAGCTCTG
GATGCTCCAGACCCGCAACGGCAAACGCACCGCCAAGGCGGCCCTGCGTATCGCCGTCGAACTCGCGGGCGAGGGCCTGA
TTTCGAAGGAAGAGGCGATCAAGCGCATCGAGCCCGGCGCGCTCGATCAGCTCCTGCACCCCACCATCGACCCGGATGCC
GAGCGGACGGTGATCGCCACCGGCCTGCCCGCCTCGCCGGGCGCGGCGGTCGGCGAGATCGTCTTCAACTCGGAGGAGGC
GGAGGCCGCCAAGAAGGCCGAGCGCAAGTGTATCCTCGTGCGCATCGAGACCTCCCCGGAGGACATCCACGGCATGCACG
CCGCCGAGGGCATCCTCACGACGCGCGGCGGCATGACCAGCCACGCGGCGGTGGTCGCCCGCGGCATGGGCAAGCCCTGC
GTCTCCGGCGTCGGCTCGATCCGGATCGACTACAAGGCCAAGACCCTGACCGTCGGCGGCGTGACCCTGAAGGCGGGTGA
CGTCATCACCGTCGACGGCTCGACCGGCCAGGTGATCCAGGGCGAGGTCAAGATGCTCCAGCCGGAGCTGTCGGGCGACT
TCGCCGCCCTGATGGAGTGGGCGGATGCGGTGCGGACCATGAAGGTGCGCACCAACGCCGACACCCCGGCCGACGCCCGC
GCCGCCCGCAAGTTCGGCGCGGAGGGGATCGGCCTGTGCCGGACCGAACACATGTTCTTCGAGGGCGACCGGATCGTGGC
GGTGCGCGAGATGATCCTCGCCGACGACACCGAAGGGCGTCGCGCGGCACTCGCCAAGATCCTGCCCTACCAGCGCCAGG
ACTTCGTCGAGCTGTTCACGATCATGTCGGGCCTGCCCGTCACGATCCGCCTGCTCGATCCGCCGCTGCACGAGTTCCTG
CCGCACACCGACGAGGAAATCCGCGAGGTGATGTCGGCCACCGGCATCAGCGAGGACAAGATCCGCAACCGCATCCGCGA
ACTCTCCGAGCACAACCCGATGCTCGGCTTCCGCGGCTGCCGCCTCGCCATCGCCTTCCCCGAAATCGCCGAGATGCAGG
CCCGGGCGATCTTCGAGGCTGCGGTGCAGGCCGCCAAGGATACCGGCGCCCCGGTGACCTCCGAAGTGATGGTGCCGCTG
GTGTTCACCCGGATGGAGTTCGACATCGTCAAGGCCCGCATCGACGCCATTGCCAAGGCCGTGACGGAGGAGACCGGCGC
GACGCTCGAATATCAGGTCGGCACGATGATCGAGCTGCCGCGCGCGGCGCTGATGGCCGGCGAGATCGCCGAGACGGCGG
AGTTCTTCTCCTTCGGCACCAACGATCTCACCCAGACCGCGCTCGGCATCTCCCGCGACGACGCCGCGACCTTCCTTGGC
CCCTATACCCAGAAGGGCATCCTCTCGGTCGATCCGTTCGTGTCGATCGATCAGGAGGGCGTGGGCGAACTGGTGAAGAT
CGGCGCCGAGCGCGGTCGCAAGACCCGCGAAGGGCTCAAGCTCGGCATCTGCGGCGAGCACGGCGGTGATCCGGCCTCGA
TCGCCTTCTGCCAGGAGGTCGGGCTCGATTACGTCTCCTGCTCGCCCTTCCGTGTGCCGATCGCCCGCCTCGCCGCGGCG
CAGGCGGCCCTCGGCAAGGACGTCTGA

Upstream 100 bases:

>100_bases
TGCGGGGCGACGCGAAGCTAATCCTTGCACTGCGACATGAGATGCGCCACGCCCGCGTGAACGGCGCTAGGGTCGCCAAA
TCCGTCCAGCGGAGAGCCTG

Downstream 100 bases:

>100_bases
GACGACGCATGACGACCCCGCGGGCATGACCCGCGGGGCCTTTCCGGAAGGCGGGGAAGCTTCGCAATAATTTTGCGGAC
AGCCCGCTGAACCCGTGTCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MTKWVYSFGDGKAEGEAAMRNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYQHGETYPAELTDAVKEALEKVGTLT
GRRFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDATVEALAKDADDERFAYDSYRRFITMYSNVVLGVEHHAFEEAL
EHYKEQKGLTLDTDLKADDWKHLIGVYKKIVRDEHGSEFPQGAEDQLWGAIGAVFDSWMIPRAKKYRELNQIPESWGTAV
NVQAMVFGNMGDTSATGVAFTRNPSTGESALYGEFLINAQGEDVVAGIRTPQDITEKARIEAKSDKPSMEKAMPESFAEL
TRIYGILEKHYRDMQDMEFTIERGKLWMLQTRNGKRTAKAALRIAVELAGEGLISKEEAIKRIEPGALDQLLHPTIDPDA
ERTVIATGLPASPGAAVGEIVFNSEEAEAAKKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGVGSIRIDYKAKTLTVGGVTLKAGDVITVDGSTGQVIQGEVKMLQPELSGDFAALMEWADAVRTMKVRTNADTPADAR
AARKFGAEGIGLCRTEHMFFEGDRIVAVREMILADDTEGRRAALAKILPYQRQDFVELFTIMSGLPVTIRLLDPPLHEFL
PHTDEEIREVMSATGISEDKIRNRIRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAKDTGAPVTSEVMVPL
VFTRMEFDIVKARIDAIAKAVTEETGATLEYQVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTALGISRDDAATFLG
PYTQKGILSVDPFVSIDQEGVGELVKIGAERGRKTREGLKLGICGEHGGDPASIAFCQEVGLDYVSCSPFRVPIARLAAA
QAALGKDV

Sequences:

>Translated_888_residues
MTKWVYSFGDGKAEGEAAMRNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYQHGETYPAELTDAVKEALEKVGTLT
GRRFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDATVEALAKDADDERFAYDSYRRFITMYSNVVLGVEHHAFEEAL
EHYKEQKGLTLDTDLKADDWKHLIGVYKKIVRDEHGSEFPQGAEDQLWGAIGAVFDSWMIPRAKKYRELNQIPESWGTAV
NVQAMVFGNMGDTSATGVAFTRNPSTGESALYGEFLINAQGEDVVAGIRTPQDITEKARIEAKSDKPSMEKAMPESFAEL
TRIYGILEKHYRDMQDMEFTIERGKLWMLQTRNGKRTAKAALRIAVELAGEGLISKEEAIKRIEPGALDQLLHPTIDPDA
ERTVIATGLPASPGAAVGEIVFNSEEAEAAKKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGVGSIRIDYKAKTLTVGGVTLKAGDVITVDGSTGQVIQGEVKMLQPELSGDFAALMEWADAVRTMKVRTNADTPADAR
AARKFGAEGIGLCRTEHMFFEGDRIVAVREMILADDTEGRRAALAKILPYQRQDFVELFTIMSGLPVTIRLLDPPLHEFL
PHTDEEIREVMSATGISEDKIRNRIRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAKDTGAPVTSEVMVPL
VFTRMEFDIVKARIDAIAKAVTEETGATLEYQVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTALGISRDDAATFLG
PYTQKGILSVDPFVSIDQEGVGELVKIGAERGRKTREGLKLGICGEHGGDPASIAFCQEVGLDYVSCSPFRVPIARLAAA
QAALGKDV
>Mature_887_residues
TKWVYSFGDGKAEGEAAMRNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYQHGETYPAELTDAVKEALEKVGTLTG
RRFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDATVEALAKDADDERFAYDSYRRFITMYSNVVLGVEHHAFEEALE
HYKEQKGLTLDTDLKADDWKHLIGVYKKIVRDEHGSEFPQGAEDQLWGAIGAVFDSWMIPRAKKYRELNQIPESWGTAVN
VQAMVFGNMGDTSATGVAFTRNPSTGESALYGEFLINAQGEDVVAGIRTPQDITEKARIEAKSDKPSMEKAMPESFAELT
RIYGILEKHYRDMQDMEFTIERGKLWMLQTRNGKRTAKAALRIAVELAGEGLISKEEAIKRIEPGALDQLLHPTIDPDAE
RTVIATGLPASPGAAVGEIVFNSEEAEAAKKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPCV
SGVGSIRIDYKAKTLTVGGVTLKAGDVITVDGSTGQVIQGEVKMLQPELSGDFAALMEWADAVRTMKVRTNADTPADARA
ARKFGAEGIGLCRTEHMFFEGDRIVAVREMILADDTEGRRAALAKILPYQRQDFVELFTIMSGLPVTIRLLDPPLHEFLP
HTDEEIREVMSATGISEDKIRNRIRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAKDTGAPVTSEVMVPLV
FTRMEFDIVKARIDAIAKAVTEETGATLEYQVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTALGISRDDAATFLGP
YTQKGILSVDPFVSIDQEGVGELVKIGAERGRKTREGLKLGICGEHGGDPASIAFCQEVGLDYVSCSPFRVPIARLAAAQ
AALGKDV

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=458, Percent_Identity=24.235807860262, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787994, Length=727, Percent_Identity=25.0343878954608, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1789193, Length=418, Percent_Identity=24.8803827751196, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI48994992, Length=436, Percent_Identity=24.7706422018349, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788726, Length=470, Percent_Identity=22.7659574468085, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96572; Mature: 96441

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYSFGDGKAEGEAAMRNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYQHGET
CCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCC
YPAELTDAVKEALEKVGTLTGRRFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDATV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EALAKDADDERFAYDSYRRFITMYSNVVLGVEHHAFEEALEHYKEQKGLTLDTDLKADDW
HHHHHCCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCEEECCCCCHHH
KHLIGVYKKIVRDEHGSEFPQGAEDQLWGAIGAVFDSWMIPRAKKYRELNQIPESWGTAV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCEE
NVQAMVFGNMGDTSATGVAFTRNPSTGESALYGEFLINAQGEDVVAGIRTPQDITEKARI
EEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHC
EAKSDKPSMEKAMPESFAELTRIYGILEKHYRDMQDMEFTIERGKLWMLQTRNGKRTAKA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHH
ALRIAVELAGEGLISKEEAIKRIEPGALDQLLHPTIDPDAERTVIATGLPASPGAAVGEI
HHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHE
VFNSEEAEAAKKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EECCHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCHH
VSGVGSIRIDYKAKTLTVGGVTLKAGDVITVDGSTGQVIQGEVKMLQPELSGDFAALMEW
HCCCCCEEEEEEEEEEEECCEEEECCCEEEEECCCCCEEECHHHHCCCCCCCHHHHHHHH
ADAVRTMKVRTNADTPADARAARKFGAEGIGLCRTEHMFFEGDRIVAVREMILADDTEGR
HHHHHEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCEEECCCEEHHHHHHHHCCCCCCH
RAALAKILPYQRQDFVELFTIMSGLPVTIRLLDPPLHEFLPHTDEEIREVMSATGISEDK
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHH
IRNRIRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAKDTGAPVTSEVMVPL
HHHHHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VFTRMEFDIVKARIDAIAKAVTEETGATLEYQVGTMIELPRAALMAGEIAETAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCC
NDLTQTALGISRDDAATFLGPYTQKGILSVDPFVSIDQEGVGELVKIGAERGRKTREGLK
CHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LGICGEHGGDPASIAFCQEVGLDYVSCSPFRVPIARLAAAQAALGKDV
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKWVYSFGDGKAEGEAAMRNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYQHGET
CCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCC
YPAELTDAVKEALEKVGTLTGRRFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDATV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EALAKDADDERFAYDSYRRFITMYSNVVLGVEHHAFEEALEHYKEQKGLTLDTDLKADDW
HHHHHCCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCEEECCCCCHHH
KHLIGVYKKIVRDEHGSEFPQGAEDQLWGAIGAVFDSWMIPRAKKYRELNQIPESWGTAV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCEE
NVQAMVFGNMGDTSATGVAFTRNPSTGESALYGEFLINAQGEDVVAGIRTPQDITEKARI
EEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHC
EAKSDKPSMEKAMPESFAELTRIYGILEKHYRDMQDMEFTIERGKLWMLQTRNGKRTAKA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHH
ALRIAVELAGEGLISKEEAIKRIEPGALDQLLHPTIDPDAERTVIATGLPASPGAAVGEI
HHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHE
VFNSEEAEAAKKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EECCHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCHH
VSGVGSIRIDYKAKTLTVGGVTLKAGDVITVDGSTGQVIQGEVKMLQPELSGDFAALMEW
HCCCCCEEEEEEEEEEEECCEEEECCCEEEEECCCCCEEECHHHHCCCCCCCHHHHHHHH
ADAVRTMKVRTNADTPADARAARKFGAEGIGLCRTEHMFFEGDRIVAVREMILADDTEGR
HHHHHEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCEEECCCEEHHHHHHHHCCCCCCH
RAALAKILPYQRQDFVELFTIMSGLPVTIRLLDPPLHEFLPHTDEEIREVMSATGISEDK
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHH
IRNRIRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAKDTGAPVTSEVMVPL
HHHHHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VFTRMEFDIVKARIDAIAKAVTEETGATLEYQVGTMIELPRAALMAGEIAETAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCC
NDLTQTALGISRDDAATFLGPYTQKGILSVDPFVSIDQEGVGELVKIGAERGRKTREGLK
CHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LGICGEHGGDPASIAFCQEVGLDYVSCSPFRVPIARLAAAQAALGKDV
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]