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The map label for this gene is sleB [H]

Identifier: 218531065

GI number: 218531065

Start: 3296864

End: 3297769

Strand: Reverse

Name: sleB [H]

Synonym: Mchl_3115

Alternate gene names: 218531065

Gene position: 3297769-3296864 (Counterclockwise)

Preceding gene: 218531066

Following gene: 218531061

Centisome position: 57.08

GC content: 70.2

Gene sequence:

>906_bases
GTGCGTACGAGGCGCGTTCGAGGCCTGGGGATCGGCCCCCGCTGGATCGTCTCGGCCGTGGTGCCGTGGATGCTGGCGAG
CGGCCTGCTGGTTTCCTTCACCGCCACCGCGGGCACCGATGCCACGCTTCGGCCCGCCCAGCTCTCGGAGCGGGATGTCA
CGGCGACCGGCGCGCTCGTGGCGTCGACCGCCTGGAGCCCGGACGTCATCGATGCGGGTCTCGTGGCGCCCGGCGCCTCC
CATGAATCCGAGCCCGGAGAAGACGGCCTGCCCGCGCTCGCCGACTCGGATGGCGCCACCCCCGCGACCCCCCGCGCCGT
CGCCCTGTCCTCAGTCACACCCGCGCCCGCCGACGCGACCCCGATCGAGGTCGCGGCGGCGAACCTCGCCCTACCCGGCT
TCTCGGCCCGTCTCGATCGCGGCCCTTCCGCCCTCGATCCGCAGATGACGGCGCCGGAGGCCAAGTCCCATTATGCCGAC
CTGATCGATCCGAATGCGATGGACCGCGAGCAGCGCTGCCTCGCCGAAGCCGTCTACTTTGAGGCGCGCAGCGAGCCCGA
GGAGGGGCAGGCCGCCGTCGCGCAGGTCATTCTGAACCGCGTGAAGAGCGGGCTCTACCCGTCCTCGGTCTGCGGTGTCG
TCTACCAGAACCGCCACCGCTTCATGGGGTGCCAGTTCTCCTTCGCTTGCGAGGGCAAGGCTTTGCGCATCACCGACGCC
GAATCCTGGCGCAGCGCGACCCGCGTCGCCAGCGCGGTGATCGACGGCCGCACGTATGTCAGCGAGGTCGGCGGCGCGAC
CCACTATCACGCCGATTACGTCCGCCCCGGCTGGTCGCGCCGGCTCAAGCGCAAGGACATGATCGGCCGGCATATTTTTT
ATCAGCTGAAGCCGAACCAGACCTGA

Upstream 100 bases:

>100_bases
GGCAACCACGTTCGCGATTAAACTCTCCTCATGAGGAACGGTCGCTCCGGCTTGGTCTAAGCTGAGGCCTCCGGACGCTC
GTGAGTGTGGAGTATCGGCG

Downstream 100 bases:

>100_bases
TGGTGTCTCAAAACGTCATCTCATCGACGACCTCATCCTGAGGTGCCGCGTGAGCGGCCTCGAAGGGTGTTCCAGAGGTC
GCTCGAATGCTGGAGGATCC

Product: cell wall hydrolase SleB

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRTRRVRGLGIGPRWIVSAVVPWMLASGLLVSFTATAGTDATLRPAQLSERDVTATGALVASTAWSPDVIDAGLVAPGAS
HESEPGEDGLPALADSDGATPATPRAVALSSVTPAPADATPIEVAAANLALPGFSARLDRGPSALDPQMTAPEAKSHYAD
LIDPNAMDREQRCLAEAVYFEARSEPEEGQAAVAQVILNRVKSGLYPSSVCGVVYQNRHRFMGCQFSFACEGKALRITDA
ESWRSATRVASAVIDGRTYVSEVGGATHYHADYVRPGWSRRLKRKDMIGRHIFYQLKPNQT

Sequences:

>Translated_301_residues
MRTRRVRGLGIGPRWIVSAVVPWMLASGLLVSFTATAGTDATLRPAQLSERDVTATGALVASTAWSPDVIDAGLVAPGAS
HESEPGEDGLPALADSDGATPATPRAVALSSVTPAPADATPIEVAAANLALPGFSARLDRGPSALDPQMTAPEAKSHYAD
LIDPNAMDREQRCLAEAVYFEARSEPEEGQAAVAQVILNRVKSGLYPSSVCGVVYQNRHRFMGCQFSFACEGKALRITDA
ESWRSATRVASAVIDGRTYVSEVGGATHYHADYVRPGWSRRLKRKDMIGRHIFYQLKPNQT
>Mature_301_residues
MRTRRVRGLGIGPRWIVSAVVPWMLASGLLVSFTATAGTDATLRPAQLSERDVTATGALVASTAWSPDVIDAGLVAPGAS
HESEPGEDGLPALADSDGATPATPRAVALSSVTPAPADATPIEVAAANLALPGFSARLDRGPSALDPQMTAPEAKSHYAD
LIDPNAMDREQRCLAEAVYFEARSEPEEGQAAVAQVILNRVKSGLYPSSVCGVVYQNRHRFMGCQFSFACEGKALRITDA
ESWRSATRVASAVIDGRTYVSEVGGATHYHADYVRPGWSRRLKRKDMIGRHIFYQLKPNQT

Specific function: Required for spore cortex hydrolysis during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: NA

Molecular weight: Translated: 32129; Mature: 32129

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRRVRGLGIGPRWIVSAVVPWMLASGLLVSFTATAGTDATLRPAQLSERDVTATGALV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
ASTAWSPDVIDAGLVAPGASHESEPGEDGLPALADSDGATPATPRAVALSSVTPAPADAT
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
PIEVAAANLALPGFSARLDRGPSALDPQMTAPEAKSHYADLIDPNAMDREQRCLAEAVYF
CHHEEEHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
EARSEPEEGQAAVAQVILNRVKSGLYPSSVCGVVYQNRHRFMGCQFSFACEGKALRITDA
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCEEEEEECCCEEEEECH
ESWRSATRVASAVIDGRTYVSEVGGATHYHADYVRPGWSRRLKRKDMIGRHIFYQLKPNQ
HHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCC
T
C
>Mature Secondary Structure
MRTRRVRGLGIGPRWIVSAVVPWMLASGLLVSFTATAGTDATLRPAQLSERDVTATGALV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
ASTAWSPDVIDAGLVAPGASHESEPGEDGLPALADSDGATPATPRAVALSSVTPAPADAT
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
PIEVAAANLALPGFSARLDRGPSALDPQMTAPEAKSHYADLIDPNAMDREQRCLAEAVYF
CHHEEEHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
EARSEPEEGQAAVAQVILNRVKSGLYPSSVCGVVYQNRHRFMGCQFSFACEGKALRITDA
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCEEEEEECCCEEEEECH
ESWRSATRVASAVIDGRTYVSEVGGATHYHADYVRPGWSRRLKRKDMIGRHIFYQLKPNQ
HHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132; 10484179 [H]