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The map label for this gene is metB [H]

Identifier: 218531034

GI number: 218531034

Start: 3265746

End: 3266963

Strand: Reverse

Name: metB [H]

Synonym: Mchl_3084

Alternate gene names: 218531034

Gene position: 3266963-3265746 (Counterclockwise)

Preceding gene: 218531036

Following gene: 218531033

Centisome position: 56.54

GC content: 72.74

Gene sequence:

>1218_bases
ATGAGCCGGTCCAACGCGCCTCACGGCACTTCCGATGCAGCCCCCTCGGAGGCGCTGTCGCCCGCGGAATGGCAGCCGGC
GACGCTGGCCGCGCAGGCGCTCGGCCGAATCGATCCGGTGACGCGGGCGGTGGTGCCGCCGCTTCATCTCTCCACCACCT
ATCTGCGCGACGCCGACAACGCCTACCGCTCCGGCTTCGTCTACGCCCGTCCCGACAACGCCACGACGCGCGAGGCGGAG
GACGTGATCGCCGCGCTCGAACGGGCGGAAGCGGGCGCCCTCCTGTTCGGCTCCGGCATGGCGGCGGCGTCGGCCGTGTT
CTGCGCGCTGGAGCGCGGCGACCACGTGATCGCGCCTGAAGTCATGTACTGGGGGCTGCGCCGCTGGCTGCGGCAGGAGG
CGCCGCGCCTCGACCTCGCGGTCGATTTCATGCCGATGGACGACCCCGAGGCCGTGCGTGCCGCGATCCGGCCCGGCAAG
ACCCGGCTCGTCTGGATCGAGACGCCGGGCAACCCGCTCTGCACGGTCGCCGACATCGCCCGCATCGCCGAGATCGCCCA
CGCCGCCGGGGCGCGGGTCGCCGTGGACTCGACCGCCGCGAGCCCGATCCTGACGCGGCCGCTGACGCTCGGGGCCGACA
TCGTCATGCATTCGGCCACCAAGATCCTGAACGGCCATTCCGACGTGGTGGCCGGCGTCCTGGCCGGGGCGCGGAAGGAT
GCGTTCTGGGATCGTCTCGTGGCGATCCGCGGCGGCTGGGGCGCGATCCTCGGTCCCTTCGAAGCTTACCTGCTGATGCG
GGGCTTGCGCACGCTGCCCTTGCGCGCGGCCGCCCAGAGCGCCGGGGCGCTCCACCTCGCGCAGCGTCTCTCCGCGCATC
CGCTCGTGAGTGCGGTGCTCTATCCGGGTCTACCCGACCATCCCGGCCACGCGGTTGCCGCGCGGCAGATGACGGGCGGG
TTCGGCTACATGCTCTCGATCCGCGTGGCCGGCGGCGAGGCGGCGGCGATCGAGACCGCCGCACGGGTGGCTCTGTGGAA
GCGGGCAACCTCGCTCGGCGGCGTGGAAAGCCTCATCGAGCACCGCGCCTCCGTCGAGGGGCCGGGCACCCCCTGCCCGC
CCGACCTGTTGCGCCTCTCCACCGGCATCGAAGATCCGGACGACCTGCTTCGCGATCTCGACGCCGCTCTGACGGGCGCG
CATGGGCCGACCGAGTGA

Upstream 100 bases:

>100_bases
CATGCGCTGTGCAATGCTCCGTCGCTCATTTCTTCGCCTTGCCCGGTCCGGCGGATTCGCTCAAGCCGGCATTGCGATCC
GACCTCGAGCGAGGAAACCC

Downstream 100 bases:

>100_bases
CGCAGTTCCGAATTGTGTGAAGGCGCGTGGCTGTTGCGGGGCTCCGTGCGTTGTCGGGTCTACGAGCCCCCAACGGGACG
AAAACCGCCATGATGAACCG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSRSNAPHGTSDAAPSEALSPAEWQPATLAAQALGRIDPVTRAVVPPLHLSTTYLRDADNAYRSGFVYARPDNATTREAE
DVIAALERAEAGALLFGSGMAAASAVFCALERGDHVIAPEVMYWGLRRWLRQEAPRLDLAVDFMPMDDPEAVRAAIRPGK
TRLVWIETPGNPLCTVADIARIAEIAHAAGARVAVDSTAASPILTRPLTLGADIVMHSATKILNGHSDVVAGVLAGARKD
AFWDRLVAIRGGWGAILGPFEAYLLMRGLRTLPLRAAAQSAGALHLAQRLSAHPLVSAVLYPGLPDHPGHAVAARQMTGG
FGYMLSIRVAGGEAAAIETAARVALWKRATSLGGVESLIEHRASVEGPGTPCPPDLLRLSTGIEDPDDLLRDLDAALTGA
HGPTE

Sequences:

>Translated_405_residues
MSRSNAPHGTSDAAPSEALSPAEWQPATLAAQALGRIDPVTRAVVPPLHLSTTYLRDADNAYRSGFVYARPDNATTREAE
DVIAALERAEAGALLFGSGMAAASAVFCALERGDHVIAPEVMYWGLRRWLRQEAPRLDLAVDFMPMDDPEAVRAAIRPGK
TRLVWIETPGNPLCTVADIARIAEIAHAAGARVAVDSTAASPILTRPLTLGADIVMHSATKILNGHSDVVAGVLAGARKD
AFWDRLVAIRGGWGAILGPFEAYLLMRGLRTLPLRAAAQSAGALHLAQRLSAHPLVSAVLYPGLPDHPGHAVAARQMTGG
FGYMLSIRVAGGEAAAIETAARVALWKRATSLGGVESLIEHRASVEGPGTPCPPDLLRLSTGIEDPDDLLRDLDAALTGA
HGPTE
>Mature_404_residues
SRSNAPHGTSDAAPSEALSPAEWQPATLAAQALGRIDPVTRAVVPPLHLSTTYLRDADNAYRSGFVYARPDNATTREAED
VIAALERAEAGALLFGSGMAAASAVFCALERGDHVIAPEVMYWGLRRWLRQEAPRLDLAVDFMPMDDPEAVRAAIRPGKT
RLVWIETPGNPLCTVADIARIAEIAHAAGARVAVDSTAASPILTRPLTLGADIVMHSATKILNGHSDVVAGVLAGARKDA
FWDRLVAIRGGWGAILGPFEAYLLMRGLRTLPLRAAAQSAGALHLAQRLSAHPLVSAVLYPGLPDHPGHAVAARQMTGGF
GYMLSIRVAGGEAAAIETAARVALWKRATSLGGVESLIEHRASVEGPGTPCPPDLLRLSTGIEDPDDLLRDLDAALTGAH
GPTE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=321, Percent_Identity=41.7445482866044, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI299473759, Length=321, Percent_Identity=38.3177570093458, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI25453487, Length=320, Percent_Identity=35, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1790375, Length=393, Percent_Identity=40.2035623409669, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789383, Length=346, Percent_Identity=31.2138728323699, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=356, Percent_Identity=38.7640449438202, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17560056, Length=354, Percent_Identity=37.5706214689266, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI71980708, Length=390, Percent_Identity=37.6923076923077, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=39.386189258312, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6321904, Length=370, Percent_Identity=30.8108108108108, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321254, Length=390, Percent_Identity=30, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=28.6697247706422, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=298, Percent_Identity=28.5234899328859, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922554, Length=407, Percent_Identity=37.3464373464373, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42629; Mature: 42498

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSNAPHGTSDAAPSEALSPAEWQPATLAAQALGRIDPVTRAVVPPLHLSTTYLRDADN
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCH
AYRSGFVYARPDNATTREAEDVIAALERAEAGALLFGSGMAAASAVFCALERGDHVIAPE
HHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEECHH
VMYWGLRRWLRQEAPRLDLAVDFMPMDDPEAVRAAIRPGKTRLVWIETPGNPLCTVADIA
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
RIAEIAHAAGARVAVDSTAASPILTRPLTLGADIVMHSATKILNGHSDVVAGVLAGARKD
HHHHHHHHCCCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHH
AFWDRLVAIRGGWGAILGPFEAYLLMRGLRTLPLRAAAQSAGALHLAQRLSAHPLVSAVL
HHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCHHHHHHH
YPGLPDHPGHAVAARQMTGGFGYMLSIRVAGGEAAAIETAARVALWKRATSLGGVESLIE
CCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
HRASVEGPGTPCPPDLLRLSTGIEDPDDLLRDLDAALTGAHGPTE
HHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRSNAPHGTSDAAPSEALSPAEWQPATLAAQALGRIDPVTRAVVPPLHLSTTYLRDADN
CCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCH
AYRSGFVYARPDNATTREAEDVIAALERAEAGALLFGSGMAAASAVFCALERGDHVIAPE
HHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEECHH
VMYWGLRRWLRQEAPRLDLAVDFMPMDDPEAVRAAIRPGKTRLVWIETPGNPLCTVADIA
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
RIAEIAHAAGARVAVDSTAASPILTRPLTLGADIVMHSATKILNGHSDVVAGVLAGARKD
HHHHHHHHCCCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHH
AFWDRLVAIRGGWGAILGPFEAYLLMRGLRTLPLRAAAQSAGALHLAQRLSAHPLVSAVL
HHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCHHHHHHH
YPGLPDHPGHAVAARQMTGGFGYMLSIRVAGGEAAAIETAARVALWKRATSLGGVESLIE
CCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
HRASVEGPGTPCPPDLLRLSTGIEDPDDLLRDLDAALTGAHGPTE
HHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]