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The map label for this gene is ispDF [H]

Identifier: 218530994

GI number: 218530994

Start: 3214564

End: 3215898

Strand: Reverse

Name: ispDF [H]

Synonym: Mchl_3044

Alternate gene names: 218530994

Gene position: 3215898-3214564 (Counterclockwise)

Preceding gene: 218531003

Following gene: 218530993

Centisome position: 55.66

GC content: 71.39

Gene sequence:

>1335_bases
ATGTCCGACCAAGCCGCGCGGCCGCCCGGACAGGAGCCGGGAAATAAATCGGTGGCGGCGGTCGTGGTCGCGGCCGGCAA
GGGGCTGCGCGTCGGCGGCGACTTACCCAAGCAATATCGCCGCGTCGGCGGCCGGGCCGTGCTGACGCGGACGCTTGCGG
CGCTGGCGCAATCGCCCCGCATCACCCGCATCCAGCCGGTGATCGCGCCGGATGCGCAGGACTTCTATCGCGAATGCCTC
GCCGATCTCGACCCGGCCCATCGTGAAAAGCTCGCCGAGCCGGTGCCGGGCGGGGCGACGCGCCAGCAATCGGTGGCGGC
CGGGCTCAAAGGGCTTGCCCGCTCGGGCGCGCCCGATCTCGTGCTCGTCCACGACGCGGCGCGGCCCTTCGTGGACGAGG
CGCTGATCGCCCGCGCGGTCGCGGCAGGCTCCGAGCACGGCGCATCGGTGCCGGGCATCACGGTCTCCGACACGATCAAG
CTCGTGGAGGAGATCGCGCCGGGCATCGGCCGCGTCCACGAAACTCCGGCTCGCGAAAATCTCCGCGCGGTGCAGACGCC
GCAGAGCTTCCGTTTCGGCCTGCTTCTCGACGCGCATCGCCGGGCGGTGGCCGAGGGCCGCGACGGCTTCACCGATGACG
GGGCGCTCGCCGAATGGGCCGGGCTGCCGGTCGTGGTGTTCGAGGGCGACGCACGCAACCGCAAGATCACCCAGGCCGCG
GACCTGATCGAGGCCGACCGGGCATTCTCCGGACGGGCTTTCTCTGAACCTGCGCCCGCGATATCGGATGACACCATGAC
CACTTACGTAACCCGCCTTGGCACCGGCTTCGACGTCCACGCCTTCACGGAGGGCGACCATGTCTGGCTCGGCGGCGTGA
AAATCCCCGCCGACCGCGGCGTGCTCGCCCATTCCGACGGCGACGTGGCGCTGCACGCCCTCACCGACGCGCTGCTCGGC
GCCATCGCCGACGGCGATATCGGCACGCACTTCCCGCCCTCGGACGAAAAGTGGCGCGGCGCGGCCTCCGACCAGTTCCT
GGCACACGCCTGCGAATTGGTGCGGGCGCGCGGCGGCAAGATCGACCATCTCGACATCACGGTGCTGGCGGAAGCTCCGC
GCATCGGTCAGCACCGCGAGGCGATCCGCGCGCGCATCGCCGAGATCGCGGGCGTGCCGCTCTCCTCGGTGTCGATCAAG
GCGACCACGACCGAGAAGCTCGGCTTCGTCGGCCGCGCCGAGGGCCTCGCCGCCCAGGCCGCCGCGACGGTGCGCCTGCC
GGAGGTTTGCGCGGAGCTGGAGACCGAGGCGGAGACCAACGAGCGCCGTTCGTGA

Upstream 100 bases:

>100_bases
GCAGACGCAAGCGCCTGCGGCGTTTGCCTTCGCGCATCTTGCCGCATAAGGACGCGGCGATCCCCATCCATCCCCAGATG
ATTTCCATTCGAGGCCCAGC

Downstream 100 bases:

>100_bases
TCGAAGACGCCGCTTTGCTGGCCCGCGCCGAGGCGCTGGTGCGGGCCTATGCGGCGGCGGGCCAACGCATCGCGACCGCC
GAGTCCTGCACCGGCGGTCT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSDQAARPPGQEPGNKSVAAVVVAAGKGLRVGGDLPKQYRRVGGRAVLTRTLAALAQSPRITRIQPVIAPDAQDFYRECL
ADLDPAHREKLAEPVPGGATRQQSVAAGLKGLARSGAPDLVLVHDAARPFVDEALIARAVAAGSEHGASVPGITVSDTIK
LVEEIAPGIGRVHETPARENLRAVQTPQSFRFGLLLDAHRRAVAEGRDGFTDDGALAEWAGLPVVVFEGDARNRKITQAA
DLIEADRAFSGRAFSEPAPAISDDTMTTYVTRLGTGFDVHAFTEGDHVWLGGVKIPADRGVLAHSDGDVALHALTDALLG
AIADGDIGTHFPPSDEKWRGAASDQFLAHACELVRARGGKIDHLDITVLAEAPRIGQHREAIRARIAEIAGVPLSSVSIK
ATTTEKLGFVGRAEGLAAQAAATVRLPEVCAELETEAETNERRS

Sequences:

>Translated_444_residues
MSDQAARPPGQEPGNKSVAAVVVAAGKGLRVGGDLPKQYRRVGGRAVLTRTLAALAQSPRITRIQPVIAPDAQDFYRECL
ADLDPAHREKLAEPVPGGATRQQSVAAGLKGLARSGAPDLVLVHDAARPFVDEALIARAVAAGSEHGASVPGITVSDTIK
LVEEIAPGIGRVHETPARENLRAVQTPQSFRFGLLLDAHRRAVAEGRDGFTDDGALAEWAGLPVVVFEGDARNRKITQAA
DLIEADRAFSGRAFSEPAPAISDDTMTTYVTRLGTGFDVHAFTEGDHVWLGGVKIPADRGVLAHSDGDVALHALTDALLG
AIADGDIGTHFPPSDEKWRGAASDQFLAHACELVRARGGKIDHLDITVLAEAPRIGQHREAIRARIAEIAGVPLSSVSIK
ATTTEKLGFVGRAEGLAAQAAATVRLPEVCAELETEAETNERRS
>Mature_443_residues
SDQAARPPGQEPGNKSVAAVVVAAGKGLRVGGDLPKQYRRVGGRAVLTRTLAALAQSPRITRIQPVIAPDAQDFYRECLA
DLDPAHREKLAEPVPGGATRQQSVAAGLKGLARSGAPDLVLVHDAARPFVDEALIARAVAAGSEHGASVPGITVSDTIKL
VEEIAPGIGRVHETPARENLRAVQTPQSFRFGLLLDAHRRAVAEGRDGFTDDGALAEWAGLPVVVFEGDARNRKITQAAD
LIEADRAFSGRAFSEPAPAISDDTMTTYVTRLGTGFDVHAFTEGDHVWLGGVKIPADRGVLAHSDGDVALHALTDALLGA
IADGDIGTHFPPSDEKWRGAASDQFLAHACELVRARGGKIDHLDITVLAEAPRIGQHREAIRARIAEIAGVPLSSVSIKA
TTTEKLGFVGRAEGLAAQAAATVRLPEVCAELETEAETNERRS

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157671913, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789103, Length=153, Percent_Identity=51.6339869281046, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=36.0869565217391, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 46754; Mature: 46623

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00626 RCC1_2 ; PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQAARPPGQEPGNKSVAAVVVAAGKGLRVGGDLPKQYRRVGGRAVLTRTLAALAQSPR
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
ITRIQPVIAPDAQDFYRECLADLDPAHREKLAEPVPGGATRQQSVAAGLKGLARSGAPDL
CEEECCEECCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
VLVHDAARPFVDEALIARAVAAGSEHGASVPGITVSDTIKLVEEIAPGIGRVHETPAREN
EEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHC
LRAVQTPQSFRFGLLLDAHRRAVAEGRDGFTDDGALAEWAGLPVVVFEGDARNRKITQAA
CHHHCCCCHHEEEEEEHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCHHHHH
DLIEADRAFSGRAFSEPAPAISDDTMTTYVTRLGTGFDVHAFTEGDHVWLGGVKIPADRG
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEECCEECCCCCC
VLAHSDGDVALHALTDALLGAIADGDIGTHFPPSDEKWRGAASDQFLAHACELVRARGGK
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
IDHLDITVLAEAPRIGQHREAIRARIAEIAGVPLSSVSIKATTTEKLGFVGRAEGLAAQA
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCHHHHH
AATVRLPEVCAELETEAETNERRS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDQAARPPGQEPGNKSVAAVVVAAGKGLRVGGDLPKQYRRVGGRAVLTRTLAALAQSPR
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
ITRIQPVIAPDAQDFYRECLADLDPAHREKLAEPVPGGATRQQSVAAGLKGLARSGAPDL
CEEECCEECCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
VLVHDAARPFVDEALIARAVAAGSEHGASVPGITVSDTIKLVEEIAPGIGRVHETPAREN
EEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHC
LRAVQTPQSFRFGLLLDAHRRAVAEGRDGFTDDGALAEWAGLPVVVFEGDARNRKITQAA
CHHHCCCCHHEEEEEEHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCHHHHH
DLIEADRAFSGRAFSEPAPAISDDTMTTYVTRLGTGFDVHAFTEGDHVWLGGVKIPADRG
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEECCEECCCCCC
VLAHSDGDVALHALTDALLGAIADGDIGTHFPPSDEKWRGAASDQFLAHACELVRARGGK
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
IDHLDITVLAEAPRIGQHREAIRARIAEIAGVPLSSVSIKATTTEKLGFVGRAEGLAAQA
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCHHHHH
AATVRLPEVCAELETEAETNERRS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA