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The map label for this gene is ilvG [H]

Identifier: 218711014

GI number: 218711014

Start: 3277464

End: 3279110

Strand: Direct

Name: ilvG [H]

Synonym: VS_3143

Alternate gene names: 218711014

Gene position: 3277464-3279110 (Clockwise)

Preceding gene: 218711012

Following gene: 218711015

Centisome position: 99.34

GC content: 48.94

Gene sequence:

>1647_bases
ATGAAAGGCGCAGAACTGGTCGTATCCGCACTCAAGCAACAAGGCATTGAGACCGTATTTGGTTACCCTGGCGGTGCCAT
CATGCCAATCTACGATGCACTCTATGACGGCGGGGTTGAACATATCCTATGTCGCCATGAGCAAGGCGCTGCAATGGCCG
CTATCGGTATGGCTCGAGCAACGCAAGACGTCGCTGTTTGTATGGCAACCTCAGGCCCGGGTGCAACTAACCTAGTCACA
GGTCTTGCTGATGCTTTTATGGATTCCATCCCACTCGTAGCGATCACAGGTCAAGTTGCTAGTTCCCACATTGGTACCGA
TGCATTCCAAGAAATGGATGTGATTGGTATGTCTCTGTCATGTACTAAACACAGCTACCTTGTTACCGATATTGAAGATC
TTGCGCCGACATTGGCTGAAGCATTTATCGTTGCAAAAGCAGGGCGTCCCGGTCCGGTTATTGTCGATATCGCTAAAGAT
GTTCAACTAGCAGAGTCCCCTGTAAAAACTCTTCCTGAATTTACACCACCCGCAATTCCTGTAGTGACAACGGATGCTAT
TGAGCAAGCTCAGTACTTCTTGTCTCAAGCGACTCGTCCTGTTTTATACGTAGGTGGTGGCGTTCAACTGGCGAAAGCAA
CCGATGCTGTTCGTGAGTTTTTACGTCTAAACCCAATGCCTGCAGTAAGCACACTAAAAGGCTTAGGCACCATTGAACGT
GACGACCCACACTACTTGGGTATGCTGGGTATGCACGGCACCAAAGCCGCTAACCTAGTGGTTCAAGAAAGTGACCTGTT
GATTGTTGTTGGCGCTCGTTTTGATGACCGAGTAACCGGCAAGCTCGACACCTTTGCACCGCACGCTAAAGTTATCCATA
TTGATATCGATGCGGCAGAGTTCAGTAAACTACGTCTTGCCGATGCACCAATTCGTGGTGACATCAACAAGATCATGCCT
CAACTGGAGCTAAGCCAAGACATCTCTTCTTGGGTTCATCACTCCGAAGGCCTTCGTAGCTCATTCAAATGGCGTTACGA
CCACCCTGGCGACCTTATCTTCGCACCTCTGTTGTTGAAACAACTGTCAGACATGATGCCTGCGAGTTCTATGGTTTCGA
CCGATGTTGGCCAACATCAGATGTGGGCAGCTCAACACATTCAGCCACGCGATCCACAAAACTTCATCACTTCAGCGGGC
TTAGGCACTATGGGCTTTGGCCTACCTGCAGCCATGGGTGCATCGGTTGGTCGTCCTAATGACCAATCTATACTTATCTC
TGGTGACGGCTCGTTCATGATGAACATTCAAGAGCTTGGTACGCTGAAGCGTCGCCAAATCCCAGTAAAAATGGTGCTGC
TTAACAACTCGCGCTTAGGCATGGTTCGCCAATGGCAATCGCTGTTCTTTGATGGCCGACACAGTGAAACCATCTTGGAT
GACAACCCGGATTTCGTCATGCTAGCGAAAGCATTTGATATCCCGGGCAAAACCATCACGCGTAAAGAAGAAGTAGAACC
AGCACTGAAAGAGATGCTAGAGAGCAAAACCGCTTACCTACTTCATGTTCTTATCGATGAAGAAGAGAACGTATGGCCAC
TAGTACCGCCAGGTGCTTCAAACAGTGAGATGTTGGAGAACACATAA

Upstream 100 bases:

>100_bases
ACATTTTCGGCTTTGCCGATTTACAGGAAGAATGGGAGCACAAGATGTGCAGAGACAATGGAAACAATTACCAAAACAAA
GGTAATACGGAGGAATTACG

Downstream 100 bases:

>100_bases
CATGAAAAGATACCTATTAGACATCAAAGCCGATGATAAGCCTGTACTACTAGAGCGTGTTCTTCGTGTTATCCGCCACC
GTGGCTTTATTGTTAAGCAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKGAELVVSALKQQGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARATQDVAVCMATSGPGATNLVT
GLADAFMDSIPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDIEDLAPTLAEAFIVAKAGRPGPVIVDIAKD
VQLAESPVKTLPEFTPPAIPVVTTDAIEQAQYFLSQATRPVLYVGGGVQLAKATDAVREFLRLNPMPAVSTLKGLGTIER
DDPHYLGMLGMHGTKAANLVVQESDLLIVVGARFDDRVTGKLDTFAPHAKVIHIDIDAAEFSKLRLADAPIRGDINKIMP
QLELSQDISSWVHHSEGLRSSFKWRYDHPGDLIFAPLLLKQLSDMMPASSMVSTDVGQHQMWAAQHIQPRDPQNFITSAG
LGTMGFGLPAAMGASVGRPNDQSILISGDGSFMMNIQELGTLKRRQIPVKMVLLNNSRLGMVRQWQSLFFDGRHSETILD
DNPDFVMLAKAFDIPGKTITRKEEVEPALKEMLESKTAYLLHVLIDEEENVWPLVPPGASNSEMLENT

Sequences:

>Translated_548_residues
MKGAELVVSALKQQGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARATQDVAVCMATSGPGATNLVT
GLADAFMDSIPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDIEDLAPTLAEAFIVAKAGRPGPVIVDIAKD
VQLAESPVKTLPEFTPPAIPVVTTDAIEQAQYFLSQATRPVLYVGGGVQLAKATDAVREFLRLNPMPAVSTLKGLGTIER
DDPHYLGMLGMHGTKAANLVVQESDLLIVVGARFDDRVTGKLDTFAPHAKVIHIDIDAAEFSKLRLADAPIRGDINKIMP
QLELSQDISSWVHHSEGLRSSFKWRYDHPGDLIFAPLLLKQLSDMMPASSMVSTDVGQHQMWAAQHIQPRDPQNFITSAG
LGTMGFGLPAAMGASVGRPNDQSILISGDGSFMMNIQELGTLKRRQIPVKMVLLNNSRLGMVRQWQSLFFDGRHSETILD
DNPDFVMLAKAFDIPGKTITRKEEVEPALKEMLESKTAYLLHVLIDEEENVWPLVPPGASNSEMLENT
>Mature_548_residues
MKGAELVVSALKQQGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARATQDVAVCMATSGPGATNLVT
GLADAFMDSIPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDIEDLAPTLAEAFIVAKAGRPGPVIVDIAKD
VQLAESPVKTLPEFTPPAIPVVTTDAIEQAQYFLSQATRPVLYVGGGVQLAKATDAVREFLRLNPMPAVSTLKGLGTIER
DDPHYLGMLGMHGTKAANLVVQESDLLIVVGARFDDRVTGKLDTFAPHAKVIHIDIDAAEFSKLRLADAPIRGDINKIMP
QLELSQDISSWVHHSEGLRSSFKWRYDHPGDLIFAPLLLKQLSDMMPASSMVSTDVGQHQMWAAQHIQPRDPQNFITSAG
LGTMGFGLPAAMGASVGRPNDQSILISGDGSFMMNIQELGTLKRRQIPVKMVLLNNSRLGMVRQWQSLFFDGRHSETILD
DNPDFVMLAKAFDIPGKTITRKEEVEPALKEMLESKTAYLLHVLIDEEENVWPLVPPGASNSEMLENT

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=24.8201438848921, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI21361361, Length=507, Percent_Identity=25.8382642998028, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=45.6204379562044, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=42.9328621908127, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=29.8747763864043, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=28.1767955801105, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=26.3636363636364, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=27.9166666666667, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=27.9166666666667, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=25.3833049403748, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=41.6666666666667, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=473, Percent_Identity=23.4672304439746, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=476, Percent_Identity=22.4789915966387, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=493, Percent_Identity=22.7180527383367, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=484, Percent_Identity=22.3140495867769, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922626, Length=546, Percent_Identity=24.3589743589744, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59275; Mature: 59275

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGAELVVSALKQQGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHC
TQDVAVCMATSGPGATNLVTGLADAFMDSIPLVAITGQVASSHIGTDAFQEMDVIGMSLS
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHCEEEE
CTKHSYLVTDIEDLAPTLAEAFIVAKAGRPGPVIVDIAKDVQLAESPVKTLPEFTPPAIP
CCCCCEEEECHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHCHHHHCCCCCCCCCC
VVTTDAIEQAQYFLSQATRPVLYVGGGVQLAKATDAVREFLRLNPMPAVSTLKGLGTIER
EEEHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
DDPHYLGMLGMHGTKAANLVVQESDLLIVVGARFDDRVTGKLDTFAPHAKVIHIDIDAAE
CCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHH
FSKLRLADAPIRGDINKIMPQLELSQDISSWVHHSEGLRSSFKWRYDHPGDLIFAPLLLK
HHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QLSDMMPASSMVSTDVGQHQMWAAQHIQPRDPQNFITSAGLGTMGFGLPAAMGASVGRPN
HHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCCHHCCCCHHHHCCCCCCCC
DQSILISGDGSFMMNIQELGTLKRRQIPVKMVLLNNSRLGMVRQWQSLFFDGRHSETILD
CCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEC
DNPDFVMLAKAFDIPGKTITRKEEVEPALKEMLESKTAYLLHVLIDEEENVWPLVPPGAS
CCCCEEEEEEEECCCCCCCCCHHHCCHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCC
NSEMLENT
CCHHHCCC
>Mature Secondary Structure
MKGAELVVSALKQQGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHC
TQDVAVCMATSGPGATNLVTGLADAFMDSIPLVAITGQVASSHIGTDAFQEMDVIGMSLS
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHCEEEE
CTKHSYLVTDIEDLAPTLAEAFIVAKAGRPGPVIVDIAKDVQLAESPVKTLPEFTPPAIP
CCCCCEEEECHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHCHHHHCCCCCCCCCC
VVTTDAIEQAQYFLSQATRPVLYVGGGVQLAKATDAVREFLRLNPMPAVSTLKGLGTIER
EEEHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
DDPHYLGMLGMHGTKAANLVVQESDLLIVVGARFDDRVTGKLDTFAPHAKVIHIDIDAAE
CCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHH
FSKLRLADAPIRGDINKIMPQLELSQDISSWVHHSEGLRSSFKWRYDHPGDLIFAPLLLK
HHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QLSDMMPASSMVSTDVGQHQMWAAQHIQPRDPQNFITSAGLGTMGFGLPAAMGASVGRPN
HHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCCHHCCCCHHHHCCCCCCCC
DQSILISGDGSFMMNIQELGTLKRRQIPVKMVLLNNSRLGMVRQWQSLFFDGRHSETILD
CCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEC
DNPDFVMLAKAFDIPGKTITRKEEVEPALKEMLESKTAYLLHVLIDEEENVWPLVPPGAS
CCCCEEEEEEEECCCCCCCCCHHHCCHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCC
NSEMLENT
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]