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The map label for this gene is thiC

Identifier: 218710984

GI number: 218710984

Start: 3242439

End: 3244388

Strand: Reverse

Name: thiC

Synonym: VS_3098

Alternate gene names: 218710984

Gene position: 3244388-3242439 (Counterclockwise)

Preceding gene: 218710985

Following gene: 218710983

Centisome position: 98.34

GC content: 46.05

Gene sequence:

>1950_bases
ATGTCGAGTCGTAAACAAGCAAGACTGGAAGCGAAGAATTTCATCGATTCTTTATCCGTACAACCTTATCCAAATTCAAA
AAAAGCTTACATCCAAGGATCTCGAGAAGATATTCAAGTCCCTGTACGAGAAATATCACTTGCTGATAGCCTTGTCGGTG
GTACCAAAAAAGAGCCTGTATTCGAACCTAATGTACCCATTCAAGTTTACGATACCTCAGGTGTTTACACTGACCCCACA
CATCAAATAGACCTGTATAGCGGCCTTCCAAAGTTGCGTGAGCAATGGATTGATGAGCGTGGTGATACGGAACTGTTAGA
CGATGTAAGCTCTGTTTACACTAAAGAACGTTTAGAAGATGAAACTCTAGACGACCTTCGTTACGGCAACCTACCTAGAA
TTCGTCGCGCGACTGACGAGCAATGTGTTACTCAGCTGCACTATGCTCGTCAGGGGATTATCACTCCTGAGATGGAGTAT
ATTGCTATACGTGAGAACATGGGGCGTCAGAAGTTTGCTGATGAGCAGCTTAACCACCAACATCCTGGCCATAACTTTGG
CGCAAATCTACCGAAAGAAATTACCCCTGAGTTTGTGCGCAAGGAGGTTGCTGAAGGTCGAGCTATTATCCCTTCAAACA
TCAACCACCCAGAATCAGAACCGATGATTATTGGCCGTAACTTCTTAGTGAAGGTGAACGCTAATATCGGTAACTCTTCA
GTAAGCTCTTCGATTGAAGAAGAAGTTGAGAAGTTAGTATGGTCAACTCGCTGGGGTGGCGATACCGTAATGGACCTTTC
TACCGGCCGTAATATTCACGAAACTCGCGAATGGATCTTACGTAACAGCCCTGTACCAATCGGTACGGTTCCTATGTATC
AAGCCCTTGAAAAAGTGAATGGTGTTGCGGAAGACCTTAACTGGGAAGTGATGCGCGATACTTTGATTGAACAAGCAGAG
CAGGGTGTTGATTACTTCACTATCCACGCAGGTTTGTTGCTTCGCTACGTTCCGATGACGGCTAAACGTGTCACCGGCAT
TGTTTCTCGTGGTGGTTCTATTATCGCGAAATGGTGTCTTGCTCATCACCAAGAGAGCTTCCTTTATACTCACTTCCGCG
AGATCTGCGAGATCTGTGCGAAGTACGATGTGGCTCTGTCATTAGGTGATGGCCTACGCCCGGGCTCTATTGCAGATGCC
AACGATGAGGCTCAATTCTCGGAGCTACGTACTCTAGGTGAGCTGACTAAAGTTGCTTGGGAATATGATGTTCAGGTGAT
CATTGAAGGACCTGGACATGTACCGATGCACTTAATCAAAGAGAATATGGACGAGCAGTTAGAACACTGCCATGAAGCAC
CTTTCTATACGTTAGGCCCGTTGACGACAGATATTGCACCGGGTTATGACCATATTACCTCTGGAATTGGCGCGGCCATG
ATTGGTTGGTACGGCTGTGCGATGCTCTGCTATGTCACACCTAAAGAGCATTTAGGTCTACCAAATAAAGAAGATGTGAA
GACTGGCCTGATTACTTACAAGCTGGCTGCACACGCAGCAGACTTGGCTAAAGGACATCCGGGTGCACAAATCCGAGATA
ACGCTTTATCAAAGGCACGTTTTGAATTCCGTTGGGAAGACCAATTCAACCTAGCTCTTGATCCTGAAACTGCGCGTTCT
TTTCATGATGAAACCTTACCGCAAGAGTCGGGGAAGGTTGCTCACTTCTGCTCAATGTGTGGACCTAAATTCTGCTCGAT
GAAGATCTCTCAGGAAGTCCGAGAGTATGCGAAAGATACAGAACAAGTAGCCGCGGATCAGGCTATCGAGATTAAGATGC
TAGATAACCCATTGGAAGGGATGCGTCAGAAATCACAAGAGTTCCGTGATACTGGGTCTGAACTTTACCACCCTGCAGTA
GGTGCAAAAGAAGCTCAATTAGAGGAATAA

Upstream 100 bases:

>100_bases
GATCACAACTGTATTTCTTACCGTGATCCCTCTTGTAGCATCTAATCCGTCAAGCATTTTAATCCCTATAAATAATGGCT
AAAAGCCAAGGAAAAATGCT

Downstream 100 bases:

>100_bases
TGACAGTGAAGATACTCATCCCATCTCAAAATATTGAGTTAACGGGAGAGGTGCAGAACTGTCTATTGGTTGCTAAGCGA
CAAGGCTTGGCAACCGATGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MSSRKQARLEAKNFIDSLSVQPYPNSKKAYIQGSREDIQVPVREISLADSLVGGTKKEPVFEPNVPIQVYDTSGVYTDPT
HQIDLYSGLPKLREQWIDERGDTELLDDVSSVYTKERLEDETLDDLRYGNLPRIRRATDEQCVTQLHYARQGIITPEMEY
IAIRENMGRQKFADEQLNHQHPGHNFGANLPKEITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
VSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAEDLNWEVMRDTLIEQAE
QGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADA
NDEAQFSELRTLGELTKVAWEYDVQVIIEGPGHVPMHLIKENMDEQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPETARS
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVAADQAIEIKMLDNPLEGMRQKSQEFRDTGSELYHPAV
GAKEAQLEE

Sequences:

>Translated_649_residues
MSSRKQARLEAKNFIDSLSVQPYPNSKKAYIQGSREDIQVPVREISLADSLVGGTKKEPVFEPNVPIQVYDTSGVYTDPT
HQIDLYSGLPKLREQWIDERGDTELLDDVSSVYTKERLEDETLDDLRYGNLPRIRRATDEQCVTQLHYARQGIITPEMEY
IAIRENMGRQKFADEQLNHQHPGHNFGANLPKEITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
VSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAEDLNWEVMRDTLIEQAE
QGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADA
NDEAQFSELRTLGELTKVAWEYDVQVIIEGPGHVPMHLIKENMDEQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPETARS
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVAADQAIEIKMLDNPLEGMRQKSQEFRDTGSELYHPAV
GAKEAQLEE
>Mature_648_residues
SSRKQARLEAKNFIDSLSVQPYPNSKKAYIQGSREDIQVPVREISLADSLVGGTKKEPVFEPNVPIQVYDTSGVYTDPTH
QIDLYSGLPKLREQWIDERGDTELLDDVSSVYTKERLEDETLDDLRYGNLPRIRRATDEQCVTQLHYARQGIITPEMEYI
AIRENMGRQKFADEQLNHQHPGHNFGANLPKEITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSSV
SSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAEDLNWEVMRDTLIEQAEQ
GVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADAN
DEAQFSELRTLGELTKVAWEYDVQVIIEGPGHVPMHLIKENMDEQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMI
GWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPETARSF
HDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVAADQAIEIKMLDNPLEGMRQKSQEFRDTGSELYHPAVG
AKEAQLEE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=634, Percent_Identity=75.3943217665615, Blast_Score=1005, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_VIBSL (B7VMV7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418605.1
- GeneID:   7162551
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3098
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 73039; Mature: 72908

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 235-235 BINDING 264-264 BINDING 293-293 BINDING 329-329 BINDING 429-429 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRKQARLEAKNFIDSLSVQPYPNSKKAYIQGSREDIQVPVREISLADSLVGGTKKEPV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHCCCCCCCC
FEPNVPIQVYDTSGVYTDPTHQIDLYSGLPKLREQWIDERGDTELLDDVSSVYTKERLED
CCCCCCEEEEECCCCCCCCCCCEEHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCH
ETLDDLRYGNLPRIRRATDEQCVTQLHYARQGIITPEMEYIAIRENMGRQKFADEQLNHQ
HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHCCC
HPGHNFGANLPKEITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
CCCCCCCCCCCHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEEEEEECCCCCHH
VSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAEDLNWEVMRDTLIEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADANDEAQFSELRTLGELTKVAW
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EYDVQVIIEGPGHVPMHLIKENMDEQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKAR
HHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQFNLALDPETARSFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDT
EEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EQVAADQAIEIKMLDNPLEGMRQKSQEFRDTGSELYHPAVGAKEAQLEE
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SSRKQARLEAKNFIDSLSVQPYPNSKKAYIQGSREDIQVPVREISLADSLVGGTKKEPV
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHCCCCCCCC
FEPNVPIQVYDTSGVYTDPTHQIDLYSGLPKLREQWIDERGDTELLDDVSSVYTKERLED
CCCCCCEEEEECCCCCCCCCCCEEHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCH
ETLDDLRYGNLPRIRRATDEQCVTQLHYARQGIITPEMEYIAIRENMGRQKFADEQLNHQ
HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHCCC
HPGHNFGANLPKEITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
CCCCCCCCCCCHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEEEEEECCCCCHH
VSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAEDLNWEVMRDTLIEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADANDEAQFSELRTLGELTKVAW
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EYDVQVIIEGPGHVPMHLIKENMDEQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKAR
HHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQFNLALDPETARSFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDT
EEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EQVAADQAIEIKMLDNPLEGMRQKSQEFRDTGSELYHPAVGAKEAQLEE
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA