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The map label for this gene is rsmD [H]

Identifier: 218710920

GI number: 218710920

Start: 3170329

End: 3170931

Strand: Direct

Name: rsmD [H]

Synonym: VS_3023

Alternate gene names: 218710920

Gene position: 3170329-3170931 (Clockwise)

Preceding gene: 218710912

Following gene: 218710921

Centisome position: 96.09

GC content: 45.77

Gene sequence:

>603_bases
ATGGTAAGACGTCGCCAGCAAAACACATCACAAAAAAAGCCATCCGGAGGCTTTGTTCGAATTATTAGTGGCTCATGGAG
AGGTAGAAAGCTGCCTGTTCATGATTTAGAAGGATTACGCCCAACCATTGACCGAGTCAAAGAAACACTCTTTAACTGGG
TGGCACAAGATATCCCACATTCGACCTGCTTGGATGTATTCGCCGGTTCTGGTGGTCTAGGTTTTGAAGCCGCTTCTCGC
CAAGCAAAAATGGTGACACTGCTCGAAATGAATCAAAAAGCGGCCAAACAGCTTTCTGATAACGCGAAAGAGCTCAAAGC
AGACAACATCAATGTAGTCAACACTGATGCTATCTCTTTTCTTAAGCAGCCAGGCACTCCTTACGATATGGTCTTTCTCG
ATCCTCCATTTCGTAAAGGCTTACTCGCAGAAACAGTACAACTGCTTGAGAGCAACGGTTGGCTTGCAGACAACGCCATC
ATTTACGTCGAGACAGAGAAAGAGCTAAAACTCGAAGCTATGCCAGAAAACTGGGAATTACATCGCGATAAGACCACTGG
CCAGTCGAGCTACCGACTGTTCAATCGAATCACTGAAGAATAG

Upstream 100 bases:

>100_bases
TTTTAGCTTGATACACTTTGTCATTGTAAATTTATTTAATACTCGAACCCACTTAAATTGGGCTCGGTATAGTATCACTT
TATTAGCGGTCAAAAAATCT

Downstream 100 bases:

>100_bases
GAGTTATTGAATGAAGTTCTTGCTTCCACTAGCAAAAGCAGCCATCGCCTTTGTTTGGTTTATCTTAATCGCCAATATTT
TCTACCCATTCCCGGGTAAT

Product: putative methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MVRRRQQNTSQKKPSGGFVRIISGSWRGRKLPVHDLEGLRPTIDRVKETLFNWVAQDIPHSTCLDVFAGSGGLGFEAASR
QAKMVTLLEMNQKAAKQLSDNAKELKADNINVVNTDAISFLKQPGTPYDMVFLDPPFRKGLLAETVQLLESNGWLADNAI
IYVETEKELKLEAMPENWELHRDKTTGQSSYRLFNRITEE

Sequences:

>Translated_200_residues
MVRRRQQNTSQKKPSGGFVRIISGSWRGRKLPVHDLEGLRPTIDRVKETLFNWVAQDIPHSTCLDVFAGSGGLGFEAASR
QAKMVTLLEMNQKAAKQLSDNAKELKADNINVVNTDAISFLKQPGTPYDMVFLDPPFRKGLLAETVQLLESNGWLADNAI
IYVETEKELKLEAMPENWELHRDKTTGQSSYRLFNRITEE
>Mature_200_residues
MVRRRQQNTSQKKPSGGFVRIISGSWRGRKLPVHDLEGLRPTIDRVKETLFNWVAQDIPHSTCLDVFAGSGGLGFEAASR
QAKMVTLLEMNQKAAKQLSDNAKELKADNINVVNTDAISFLKQPGTPYDMVFLDPPFRKGLLAETVQLLESNGWLADNAI
IYVETEKELKLEAMPENWELHRDKTTGQSSYRLFNRITEE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=188, Percent_Identity=59.5744680851064, Blast_Score=233, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22618; Mature: 22618

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRRQQNTSQKKPSGGFVRIISGSWRGRKLPVHDLEGLRPTIDRVKETLFNWVAQDIPH
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCH
STCLDVFAGSGGLGFEAASRQAKMVTLLEMNQKAAKQLSDNAKELKADNINVVNTDAISF
HHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHH
LKQPGTPYDMVFLDPPFRKGLLAETVQLLESNGWLADNAIIYVETEKELKLEAMPENWEL
HHCCCCCEEEEEECCCHHCCHHHHHHHHHHCCCCEECCEEEEEECCCCEEEEECCCCCEE
HRDKTTGQSSYRLFNRITEE
CCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVRRRQQNTSQKKPSGGFVRIISGSWRGRKLPVHDLEGLRPTIDRVKETLFNWVAQDIPH
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCH
STCLDVFAGSGGLGFEAASRQAKMVTLLEMNQKAAKQLSDNAKELKADNINVVNTDAISF
HHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHH
LKQPGTPYDMVFLDPPFRKGLLAETVQLLESNGWLADNAIIYVETEKELKLEAMPENWEL
HHCCCCCEEEEEECCCHHCCHHHHHHHHHHCCCCEECCEEEEEECCCCEEEEECCCCCEE
HRDKTTGQSSYRLFNRITEE
CCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]