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The map label for this gene is lexA [H]

Identifier: 218710904

GI number: 218710904

Start: 3157250

End: 3157870

Strand: Reverse

Name: lexA [H]

Synonym: VS_3006

Alternate gene names: 218710904

Gene position: 3157870-3157250 (Counterclockwise)

Preceding gene: 218710905

Following gene: 218710903

Centisome position: 95.71

GC content: 46.38

Gene sequence:

>621_bases
ATGAAGCCGTTAACGCCCCGCCAGCAACAAGTTTTTGACCTTATCAAAAGTAAGATCGAAGATTGCGGTATGCCACCGAC
TCGTGCAGAAATCGCACGTGAACTTGGCTTCCGCTCTGCAAATGCTGCAGAAGAACATTTGAAAGCTCTTGCTCGTAAAG
AAGCGATTGAGATTATCCCGGGTGCGTCTCGCGGTATTCGTATTTTGCTTGAAGATGCAGCGAACGAAGAGCAAGGTTTA
CCATTAATCGGTCAGGTTGCCGCTGGCGAGCCTATTTTGGCTCAAGAGCATGTAGAAATGCACTACCAAGTTGACCCAGG
TATGTTTAAGCCACAAGCTGACTTCTTACTTCGCGTAAATGGCGAAAGTATGAAAGACATCGGTATTATGGATGGCGATT
TACTGGCCGTGCATAAAACACAAGACGTGCGTGATGGACAAGTTGTCGTGGCTCGTGTCGACGATGATGTAACGGTAAAA
CGCCTAGAGCGCAAAGGTTCTACCGTTCTGTTACATGCTGAAAATGAAGAGTTTTCTCCAATTCACGTGGACTTAGAATC
CCAACACTTGTCTATTGAAGGACTTGCGGTTGGTATTATTCGTAACACGGACTGGATGTAG

Upstream 100 bases:

>100_bases
TTGTAATTCGCTAAAAATGAACTAAAAACAACCAATTGCTTTGCTTTTGTTCAGTTACTGGATATACTCACAGTCAACTG
TATAAAAAGACAGGTGAATC

Downstream 100 bases:

>100_bases
TCAGTGCAAACAGCTTATTGATATTTACTCTCAATAACTTGTTATTGTAATTGATATTCATTATCATCTTCTTTGTCGAG
ATACAAGGAAGATGATGATG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKPLTPRQQQVFDLIKSKIEDCGMPPTRAEIARELGFRSANAAEEHLKALARKEAIEIIPGASRGIRILLEDAANEEQGL
PLIGQVAAGEPILAQEHVEMHYQVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVK
RLERKGSTVLLHAENEEFSPIHVDLESQHLSIEGLAVGIIRNTDWM

Sequences:

>Translated_206_residues
MKPLTPRQQQVFDLIKSKIEDCGMPPTRAEIARELGFRSANAAEEHLKALARKEAIEIIPGASRGIRILLEDAANEEQGL
PLIGQVAAGEPILAQEHVEMHYQVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVK
RLERKGSTVLLHAENEEFSPIHVDLESQHLSIEGLAVGIIRNTDWM
>Mature_206_residues
MKPLTPRQQQVFDLIKSKIEDCGMPPTRAEIARELGFRSANAAEEHLKALARKEAIEIIPGASRGIRILLEDAANEEQGL
PLIGQVAAGEPILAQEHVEMHYQVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVK
RLERKGSTVLLHAENEEFSPIHVDLESQHLSIEGLAVGIIRNTDWM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=206, Percent_Identity=72.8155339805825, Blast_Score=303, Evalue=4e-84,
Organism=Escherichia coli, GI1787431, Length=124, Percent_Identity=33.0645161290323, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 22826; Mature: 22826

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLTPRQQQVFDLIKSKIEDCGMPPTRAEIARELGFRSANAAEEHLKALARKEAIEIIP
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECC
GASRGIRILLEDAANEEQGLPLIGQVAAGEPILAQEHVEMHYQVDPGMFKPQADFLLRVN
CCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEECCCEEEEEEECCCCCCCCCCEEEEEC
GESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVKRLERKGSTVLLHAENEEFSP
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCE
IHVDLESQHLSIEGLAVGIIRNTDWM
EEEEECCCEEEECEEEEEEEECCCCC
>Mature Secondary Structure
MKPLTPRQQQVFDLIKSKIEDCGMPPTRAEIARELGFRSANAAEEHLKALARKEAIEIIP
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECC
GASRGIRILLEDAANEEQGLPLIGQVAAGEPILAQEHVEMHYQVDPGMFKPQADFLLRVN
CCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEECCCEEEEEEECCCCCCCCCCEEEEEC
GESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVKRLERKGSTVLLHAENEEFSP
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCE
IHVDLESQHLSIEGLAVGIIRNTDWM
EEEEECCCEEEECEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA