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The map label for this gene is trmA

Identifier: 218710898

GI number: 218710898

Start: 3150741

End: 3151850

Strand: Direct

Name: trmA

Synonym: VS_3000

Alternate gene names: 218710898

Gene position: 3150741-3151850 (Clockwise)

Preceding gene: 218710894

Following gene: 218710901

Centisome position: 95.5

GC content: 45.32

Gene sequence:

>1110_bases
ATGGCGAATTTAGAAGTAAACCCGCAACGCTACCAAGAACAACTGGCAGAAAAGACTGAGCGTCTTACTGAAATGTTCTC
AGAATATAATGTGCCTGAGCTGGAAGTGTATGAATCTCCAGAACAACACTACCGCATGCGTGCAGAGTTCCGCGTGTGGC
ATGAAGGTGACGATATGTATTACGTCATGTTCAATCAAGAGACCAAAGAAAAATACCGCGTAGACCAGTTCCCAGCGGCT
AGCCGCCTGATCAACGACCTAATGCCTCTATTAACGGATGCAATGAAGGACAACCACTCTCTACGCCACAAACTATTCCA
AGTAGACTTCCTTTCTACATTAAGCGGCGAGATTTTGGTGTCACTGCTTTACCACCGCCAATTGGGTGAACAGTGGATTC
AAGATGCTAAAGCACTTAAACAGCAATTAAACGATGAAGGTTTTAACCTAAACCTGATCGGCCGTGCGCGTAAGATGAAA
ATCGTACTGGACCGCGACTACGTTATCGAAAAGCTAGATGTGAATGGTGATAGCTACATCTACCAGCAAGTTGAGAACAG
CTTTACTCAACCAAACGGTAAAGTAGCAGAGAAAATGCTGGAGTGGGCTGTCGACTGTACTCAAGACAGCAAAGGCGACT
TGCTTGAACTTTACTGTGGTAACGGTAACTTCTCACTAGCACTGGCACAGAACTTCGAACGTGTACTGGCAACAGAACTA
GCGAAGCCGTCAGTAGAATCTGCGCAATACAACATCGCAGCCAACAAGATTGAAAATGTTCAGATCATCCGCATGTCTGC
GGAAGATTTTACCGTAGCAATGGAAGGCAAGCGTGAATTCCGTCGTCTACAGCAAGCGAACATCGATCTTAAGAGCTACA
ACTGCAACACTATCTTTGTTGATCCACCGCGTTCAGGTATGGATGTCGATACTTGTAAGATGGTTCAAGGCTACGAGCGT
ATCATGTACATCTCTTGTAACCCTGAGACATTGAAAGAGAACCTAGACATTCTAAGCGAAACACACGACATCACCCGTTT
TGCTCTATTTGACCAGTTCCCTTACACACACCATATGGAAGCAGGTGTATTCCTAGAGCGTAAAGCGTAA

Upstream 100 bases:

>100_bases
CGTCTATTTAGACTGAATGGCTCTCAACACTGGACAAGCGCCTGTGATTGAATAAAATCTGCGCTCTTGATTTAAAAGCA
CGATAGCAAAAGGCATAACA

Downstream 100 bases:

>100_bases
ACCTCGCTCTCTAAGTCGGAGCTAAGCACCAGATACAAAAAAACGAGCCAATTGGCTCGTTTTTTTATTTATACGTTGGT
TTAAGCGGTTTTACCGATAA

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MANLEVNPQRYQEQLAEKTERLTEMFSEYNVPELEVYESPEQHYRMRAEFRVWHEGDDMYYVMFNQETKEKYRVDQFPAA
SRLINDLMPLLTDAMKDNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLGEQWIQDAKALKQQLNDEGFNLNLIGRARKMK
IVLDRDYVIEKLDVNGDSYIYQQVENSFTQPNGKVAEKMLEWAVDCTQDSKGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVESAQYNIAANKIENVQIIRMSAEDFTVAMEGKREFRRLQQANIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYER
IMYISCNPETLKENLDILSETHDITRFALFDQFPYTHHMEAGVFLERKA

Sequences:

>Translated_369_residues
MANLEVNPQRYQEQLAEKTERLTEMFSEYNVPELEVYESPEQHYRMRAEFRVWHEGDDMYYVMFNQETKEKYRVDQFPAA
SRLINDLMPLLTDAMKDNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLGEQWIQDAKALKQQLNDEGFNLNLIGRARKMK
IVLDRDYVIEKLDVNGDSYIYQQVENSFTQPNGKVAEKMLEWAVDCTQDSKGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVESAQYNIAANKIENVQIIRMSAEDFTVAMEGKREFRRLQQANIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYER
IMYISCNPETLKENLDILSETHDITRFALFDQFPYTHHMEAGVFLERKA
>Mature_368_residues
ANLEVNPQRYQEQLAEKTERLTEMFSEYNVPELEVYESPEQHYRMRAEFRVWHEGDDMYYVMFNQETKEKYRVDQFPAAS
RLINDLMPLLTDAMKDNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLGEQWIQDAKALKQQLNDEGFNLNLIGRARKMKI
VLDRDYVIEKLDVNGDSYIYQQVENSFTQPNGKVAEKMLEWAVDCTQDSKGDLLELYCGNGNFSLALAQNFERVLATELA
KPSVESAQYNIAANKIENVQIIRMSAEDFTVAMEGKREFRRLQQANIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYERI
MYISCNPETLKENLDILSETHDITRFALFDQFPYTHHMEAGVFLERKA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=215, Percent_Identity=26.9767441860465, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI51173878, Length=215, Percent_Identity=26.9767441860465, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1790403, Length=360, Percent_Identity=63.3333333333333, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789148, Length=301, Percent_Identity=27.2425249169435, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666579, Length=215, Percent_Identity=25.1162790697674, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_VIBSL (B7VM88)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418519.1
- ProteinModelPortal:   B7VM88
- SMR:   B7VM88
- GeneID:   7162620
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3000
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 43078; Mature: 42947

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 326-326 BINDING 190-190 BINDING 218-218 BINDING 223-223 BINDING 239-239 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLEVNPQRYQEQLAEKTERLTEMFSEYNVPELEVYESPEQHYRMRAEFRVWHEGDDMY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEEEEECCCCEE
YVMFNQETKEKYRVDQFPAASRLINDLMPLLTDAMKDNHSLRHKLFQVDFLSTLSGEILV
EEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLGEQWIQDAKALKQQLNDEGFNLNLIGRARKMKIVLDRDYVIEKLDVNGDSYI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCEEEEEEECCCCHHH
YQQVENSFTQPNGKVAEKMLEWAVDCTQDSKGDLLELYCGNGNFSLALAQNFERVLATEL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHH
AKPSVESAQYNIAANKIENVQIIRMSAEDFTVAMEGKREFRRLQQANIDLKSYNCNTIFV
CCCCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCHHHHHHHHHCCCEEEEECCCEEEE
DPPRSGMDVDTCKMVQGYERIMYISCNPETLKENLDILSETHDITRFALFDQFPYTHHME
CCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVFLERKA
CCEEEEECC
>Mature Secondary Structure 
ANLEVNPQRYQEQLAEKTERLTEMFSEYNVPELEVYESPEQHYRMRAEFRVWHEGDDMY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEEEEECCCCEE
YVMFNQETKEKYRVDQFPAASRLINDLMPLLTDAMKDNHSLRHKLFQVDFLSTLSGEILV
EEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLGEQWIQDAKALKQQLNDEGFNLNLIGRARKMKIVLDRDYVIEKLDVNGDSYI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCEEEEEEECCCCHHH
YQQVENSFTQPNGKVAEKMLEWAVDCTQDSKGDLLELYCGNGNFSLALAQNFERVLATEL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHH
AKPSVESAQYNIAANKIENVQIIRMSAEDFTVAMEGKREFRRLQQANIDLKSYNCNTIFV
CCCCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCHHHHHHHHHCCCEEEEECCCEEEE
DPPRSGMDVDTCKMVQGYERIMYISCNPETLKENLDILSETHDITRFALFDQFPYTHHME
CCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVFLERKA
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA