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The map label for this gene is purD [H]

Identifier: 218710868

GI number: 218710868

Start: 3111245

End: 3112534

Strand: Direct

Name: purD [H]

Synonym: VS_2952

Alternate gene names: 218710868

Gene position: 3111245-3112534 (Clockwise)

Preceding gene: 218710867

Following gene: 218710871

Centisome position: 94.3

GC content: 49.77

Gene sequence:

>1290_bases
ATGCGAGTTTTAATCATCGGTGCTGGCGGTAGAGAACATGCTTTGGGTTGGAAAGCAGCACAAAACCCAAACGTTGAAAC
AGTATTCATTGCTCCAGGTAACGCAGGTACTGCGCTTGAGCCTAAACTTGAGAACGTAAACATCGGTGTTGAAGACATCG
CAGGTTTAGTCGCGTTTGCTCAAGAGAAAAAAATCGAACTGACTATCGTTGGCCCTGAAGCACCATTGGTTATTGGTGTG
GTTGATGCATTCCGCGAAGTTGGCCTACCAATCTTTGGGCCAACTCAAGCGGCAGCTCAGCTTGAAGGCTCTAAAGCATT
CACCAAAGACTTCCTAGCTCGTCATGAGATCCCAACAGGTTACTACTCAAACTTCACTGAGATTGAGCCAGCTATCGCTT
ACGTTCGTGAGCAAGGTGCTCCAATCGTAGTAAAAGCTGACGGTCTTGCGGCGGGTAAAGGCGTTATCGTTGCGATGACA
CTTGAAGAAGCTGAAGACGCAATCAAAGACATGCTAGCAGGCAACGCATTTGGCGAAGCAGGCAGCCGCGTGGTAATTGA
AGAGTTCCTTGAAGGCGAAGAAGCAAGCTTCATCGTAATGGTTGACGGTTCTAGCGTACTTCCTATGGCCACCAGCCAAG
ATCACAAACGTGTTGGCGACAAAGACACTGGCCCTAATACTGGCGGCATGGGTGCTTACTCTCCAGCTCCAGTGGTAACT
CCTGAAATCCATAACCGCATCCTTGAGGAAGTTATCTATCCAACGGTACGTGGTATGGACGCAGAAGGCGCACCTTACAC
TGGTTTCCTTTACGCAGGACTAATGATCGATGCTGACGGCACACCTAAGGTTATCGAATACAACTGCCGCTTTGGCGACC
CTGAAACGCAACCTATCATGATGCGTATGGAGTCAGACCTTGTTGAACTTTGCTTAATGGCTATCGATGAGAAGCTAGAC
GAAGCAGAATCAAAGTGGGACCCACGCGCTTCTATCGGTGTTGTTCTTGCTGCTGGCGGTTACCCTGCTGACTACGCAAA
AGGTGACGTTATTTCACTGCCAACCAGCGAAGTTGAAGGCCAAAAGGTTTTCCACGCGGGTACGACAAATAACGAAGCTG
GCGACGTTGTGACAAACGGTGGCCGTGTACTTTGTGCAACGGCACTGGGCAATACGGTTTCTGAAGCTCAGGAGCGCGCT
TACGCGTTGACGAAGCAAGTTAGCTGGAATGGTATGTTCCACCGCAATGACATCGGTTACCGTGCGATTGCGCGCGAGCA
AGAACAGTAA

Upstream 100 bases:

>100_bases
ATGGGCTCAATTGCTGCCTAGCCACATCACCAACTACTTTGAGCAAGACTTATAACTTTGTTTTTTAAATATTTAGATAT
TTGAGATTAAGGATTTTACA

Downstream 100 bases:

>100_bases
TCAATCTACGACTCGCTTATGTTCGAATGACCGCAAGCAAGTAACAACAGAAAAGGCGCCTCGATTGGCGCCTTTTTTAT
TGCTTAATCTGTTCTGGGGT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVNIGVEDIAGLVAFAQEKKIELTIVGPEAPLVIGV
VDAFREVGLPIFGPTQAAAQLEGSKAFTKDFLARHEIPTGYYSNFTEIEPAIAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGSSVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPTSEVEGQKVFHAGTTNNEAGDVVTNGGRVLCATALGNTVSEAQERA
YALTKQVSWNGMFHRNDIGYRAIAREQEQ

Sequences:

>Translated_429_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVNIGVEDIAGLVAFAQEKKIELTIVGPEAPLVIGV
VDAFREVGLPIFGPTQAAAQLEGSKAFTKDFLARHEIPTGYYSNFTEIEPAIAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGSSVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPTSEVEGQKVFHAGTTNNEAGDVVTNGGRVLCATALGNTVSEAQERA
YALTKQVSWNGMFHRNDIGYRAIAREQEQ
>Mature_429_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVNIGVEDIAGLVAFAQEKKIELTIVGPEAPLVIGV
VDAFREVGLPIFGPTQAAAQLEGSKAFTKDFLARHEIPTGYYSNFTEIEPAIAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGSSVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPTSEVEGQKVFHAGTTNNEAGDVVTNGGRVLCATALGNTVSEAQERA
YALTKQVSWNGMFHRNDIGYRAIAREQEQ

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=430, Percent_Identity=48.8372093023256, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=430, Percent_Identity=48.8372093023256, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=430, Percent_Identity=48.8372093023256, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI28416899, Length=430, Percent_Identity=48.8372093023256, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=75.7009345794392, Blast_Score=665, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.1764705882353, Blast_Score=307, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=43.9189189189189, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=43.953488372093, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=43.953488372093, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45704; Mature: 45704

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVNIGVEDIAGLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QEKKIELTIVGPEAPLVIGVVDAFREVGLPIFGPTQAAAQLEGSKAFTKDFLARHEIPTG
CCCEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
YYSNFTEIEPAIAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEDAIKDMLAGNAFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGSSVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRASIGVVLAAGGYPADYAKGDVISLPTSEVEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCC
QKVFHAGTTNNEAGDVVTNGGRVLCATALGNTVSEAQERAYALTKQVSWNGMFHRNDIGY
CEEEECCCCCCCCCCEEECCCEEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCEEECCCCH
RAIAREQEQ
HHHHCCCCC
>Mature Secondary Structure
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVNIGVEDIAGLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QEKKIELTIVGPEAPLVIGVVDAFREVGLPIFGPTQAAAQLEGSKAFTKDFLARHEIPTG
CCCEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
YYSNFTEIEPAIAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEDAIKDMLAGNAFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGSSVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRASIGVVLAAGGYPADYAKGDVISLPTSEVEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCC
QKVFHAGTTNNEAGDVVTNGGRVLCATALGNTVSEAQERAYALTKQVSWNGMFHRNDIGY
CEEEECCCCCCCCCCEEECCCEEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCEEECCCCH
RAIAREQEQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]