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The map label for this gene is dusB [H]

Identifier: 218710863

GI number: 218710863

Start: 3106238

End: 3107215

Strand: Direct

Name: dusB [H]

Synonym: VS_2947

Alternate gene names: 218710863

Gene position: 3106238-3107215 (Clockwise)

Preceding gene: 218710862

Following gene: 218710864

Centisome position: 94.15

GC content: 46.52

Gene sequence:

>978_bases
ATGACATTTTTGAAAATCGGAAATTATCAACTTAAGAACAATCTAATCGTCGCTCCTATGGCTGGCGTAACGGATAGACC
ATTCCGTGAGTTGTGTCTTCGTTACGGTGCGGGGATGGCAGTCAGTGAAATGATGTCCTCCAATCCGAAAGTTTGGAAAA
CGTCAAAATCTCAGCAGCGTATGGTACATGAAGGCGAATCGGGCATTCGTTCAGTACAAATCGCTGGTGCAGATCCACAG
CTTATGGCCGAGGCTGCTCAATTCAATGTTGCTAACGGTGCGCAAATCATCGATATCAATATGGGTTGCCCAGCAAAAAA
AGTGAATAAGAAGCTTGCGGGCTCAGCCCTGCTTCAGCATCCAGAACTCATTGAAGATATTCTGAAGGCTGTGGTAAATG
CTGTCGACGTTCCAGTAACGTTGAAAACGCGCACAGGCTGGGATACAGACAATAGAAACTGTGTCCAAATCGCGAAAATA
GCCGAAGACTGCGGCATACAAGCTCTTGCCCTTCACGGGAGAACTCGCGCTTGTATGTACAAAGGTGAGGCAGAATACAA
ACACATTAAAGCAGCGAAACAAGCAGTATCTATTCCGGTTATCGCTAACGGTGATATCGATAGTCCGGAAAAAGCGAAGT
TTGTGCTGGAGTACACCGGCGCTGATGCTTTAATGATAGGTCGACCTGCCCAAGGGCGTCCTTGGATTTTTAACGAAATC
CTACACTATTTGGAAAACGGCACCACGATGGACCCACTCCCGATTTCGGAAGTGAAAGACATCATGCTTGGTCATGTGCT
CGCTCTACATGAATTTTATGGAGAGTTTTTAGGCCCTCGCATCGCTCGTAAGCATGTGGGTTGGTATCTAAAAGAGCATG
AACAAGCGAGTGAGTTTCGCCGTACCTTCAACGCATTCGAAGCAGGTGATCTGCAGCTTGAAGCGCTAGAAGGTTTTTTT
GATAACGTTGCACCATAA

Upstream 100 bases:

>100_bases
TAAACGAATTACAAAAACAAATTGTAAATGCTCAAATATTAGTCTTTTCACGCAGCGAAAAAATGCGTAAAATGCGCGCC
CTTGCTGGTACAGAACTGTG

Downstream 100 bases:

>100_bases
TTACGAGAAGAGCTAGACCGAATATGTTCGAACAAAATCTGACTTCAGAAGCATTGACAGTAACTACAGTTACATCACAA
GACCAAATCACGCAGAAGCC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTFLKIGNYQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPKVWKTSKSQQRMVHEGESGIRSVQIAGADPQ
LMAEAAQFNVANGAQIIDINMGCPAKKVNKKLAGSALLQHPELIEDILKAVVNAVDVPVTLKTRTGWDTDNRNCVQIAKI
AEDCGIQALALHGRTRACMYKGEAEYKHIKAAKQAVSIPVIANGDIDSPEKAKFVLEYTGADALMIGRPAQGRPWIFNEI
LHYLENGTTMDPLPISEVKDIMLGHVLALHEFYGEFLGPRIARKHVGWYLKEHEQASEFRRTFNAFEAGDLQLEALEGFF
DNVAP

Sequences:

>Translated_325_residues
MTFLKIGNYQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPKVWKTSKSQQRMVHEGESGIRSVQIAGADPQ
LMAEAAQFNVANGAQIIDINMGCPAKKVNKKLAGSALLQHPELIEDILKAVVNAVDVPVTLKTRTGWDTDNRNCVQIAKI
AEDCGIQALALHGRTRACMYKGEAEYKHIKAAKQAVSIPVIANGDIDSPEKAKFVLEYTGADALMIGRPAQGRPWIFNEI
LHYLENGTTMDPLPISEVKDIMLGHVLALHEFYGEFLGPRIARKHVGWYLKEHEQASEFRRTFNAFEAGDLQLEALEGFF
DNVAP
>Mature_324_residues
TFLKIGNYQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPKVWKTSKSQQRMVHEGESGIRSVQIAGADPQL
MAEAAQFNVANGAQIIDINMGCPAKKVNKKLAGSALLQHPELIEDILKAVVNAVDVPVTLKTRTGWDTDNRNCVQIAKIA
EDCGIQALALHGRTRACMYKGEAEYKHIKAAKQAVSIPVIANGDIDSPEKAKFVLEYTGADALMIGRPAQGRPWIFNEIL
HYLENGTTMDPLPISEVKDIMLGHVLALHEFYGEFLGPRIARKHVGWYLKEHEQASEFRRTFNAFEAGDLQLEALEGFFD
NVAP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=33.0472103004292, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=33.0472103004292, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI31742496, Length=310, Percent_Identity=28.7096774193548, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40807366, Length=278, Percent_Identity=28.7769784172662, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=31.3492063492063, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=67.601246105919, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=34.6320346320346, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693211, Length=308, Percent_Identity=26.6233766233766, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=33.4883720930233, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=32.1285140562249, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=312, Percent_Identity=25, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=235, Percent_Identity=24.6808510638298, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=31.6872427983539, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24580595, Length=263, Percent_Identity=29.277566539924, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920448, Length=263, Percent_Identity=29.277566539924, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921524, Length=251, Percent_Identity=29.0836653386454, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45549423, Length=250, Percent_Identity=29.6, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35907; Mature: 35776

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLKIGNYQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPKVWKTSKSQQR
CCEEEECCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHH
MVHEGESGIRSVQIAGADPQLMAEAAQFNVANGAQIIDINMGCPAKKVNKKLAGSALLQH
HHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
PELIEDILKAVVNAVDVPVTLKTRTGWDTDNRNCVQIAKIAEDCGIQALALHGRTRACMY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEE
KGEAEYKHIKAAKQAVSIPVIANGDIDSPEKAKFVLEYTGADALMIGRPAQGRPWIFNEI
CCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHH
LHYLENGTTMDPLPISEVKDIMLGHVLALHEFYGEFLGPRIARKHVGWYLKEHEQASEFR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RTFNAFEAGDLQLEALEGFFDNVAP
HHHHCCCCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure 
TFLKIGNYQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPKVWKTSKSQQR
CEEEECCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHH
MVHEGESGIRSVQIAGADPQLMAEAAQFNVANGAQIIDINMGCPAKKVNKKLAGSALLQH
HHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
PELIEDILKAVVNAVDVPVTLKTRTGWDTDNRNCVQIAKIAEDCGIQALALHGRTRACMY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEE
KGEAEYKHIKAAKQAVSIPVIANGDIDSPEKAKFVLEYTGADALMIGRPAQGRPWIFNEI
CCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHH
LHYLENGTTMDPLPISEVKDIMLGHVLALHEFYGEFLGPRIARKHVGWYLKEHEQASEFR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RTFNAFEAGDLQLEALEGFFDNVAP
HHHHCCCCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]