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The map label for this gene is hcaT [H]

Identifier: 218710855

GI number: 218710855

Start: 3096298

End: 3097494

Strand: Reverse

Name: hcaT [H]

Synonym: VS_2939

Alternate gene names: 218710855

Gene position: 3097494-3096298 (Counterclockwise)

Preceding gene: 218710866

Following gene: 218710854

Centisome position: 93.88

GC content: 47.03

Gene sequence:

>1197_bases
ATGTTTAGCCCCTCTCCTTACGGATGGATATCTCAGTATTTTCTTGGTTTCTTTTTTGCGTATGGTGTGTACTTGCCATT
TTGGGCGTTGTGGTTTGAAGACCAAGGCGTTTCTGCAGGGGACATCGGTGTATTGATTGGTATTGGTTTTGCGACTCGCT
GTGTTGCTAACCTTGTGATTACGCCGCGCATCCATAACGTAGAGAATCTTTTGCCCGCTCTGCGCTGGTTGAGCTTCGCC
TCCTTGTTATTTGTTGGTTTCCACTTCTTTACTGGAGGCAGTTTTCTATTGATGCTGTTGGCAACAGTATTGTTTAACCT
GTGTTGTGGGCCGGTTATCCCTCTTTCTGACGCAATGGCTAACTATTACAGTCGTCTTAAGATGCTTGACTATGGACGCA
CTCGTTTATGGGGTTCGATTGCTTTTATTGCGGGTTCAACCGTGGTGGGTTATTTAGTCGCACAGTTCGGCACTGATATG
ATTTTGTATACAGCGCTTGTTGGTGTGCTGTTGTCGTTAGTGTTGGCGATGAGAAACCCTAACGTGATGCCAGTAACGCA
ATCTGAGAAACAAGCAGTGCGACCAAAATTAGGTGAGTTACTGCGTGAGTCGTCTGTGGTTAAATTTTTGGCCTTGATGG
CGTTGCTGCAAGGTAGCCATGCGGCTTACTACAGTTTTAGTGCGATTTACTGGAAAGAAGCCGGTCACTCAGAAGCGATT
ATTGGTTATTTATGGAGCCTAGGCGTTGTTGCTGAGGTGGCTGTGTTTGCTCTCAGTAAGCGCTTATTCTCTGGCTGGTC
AATGCGTACTCTATTCGTGGTAGCTGCAATCGGTGTGATGGCTCGTTGGGGGATTACCGCATCAACAACGGCGATTTTCG
CCTTGGTTATGGTGCAAATGCTGCATGGTGTGACGTTTGCTATGGCGCACATCGCAGCCATTCAATACATTCAATCAGAA
GAACAGAATAAAATGGTCGCGCTGCAAGCTCTGTATAACGCGATCCCATTGGGGGCTGTTATCGCACTAATGACGACCTT
AAGTGGTTGGGGCTATGAGCTTTGGGGGGCAAACATCTTCTGGGGTATGGCCGCGATGGGGGCTCTTGCTCTGTTCATTA
AGTTGGACGAGAGAAGTTCAGTGGTTGAGATTAATCAGTCAGGTTCGGAACAATTAGAATCTAACAGCAAATGCTGA

Upstream 100 bases:

>100_bases
TGAATATAGGAATGCTATCCCAGTAATAATTCCCTTTTTCATCCTACTCTAGATAGGCATAATTGGAGCCGCCTTTTTCC
GCACACTTTATAGGTTATTT

Downstream 100 bases:

>100_bases
TAATTAGAGTCTGAAGCACAGAATTTAAGCGATTCGATTGAGTTAATGACCCATCCTTAGTTAAATGAAACCTCTCCCTT
ATGGAGAGGTTTTTTTATGG

Product: putative 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MFSPSPYGWISQYFLGFFFAYGVYLPFWALWFEDQGVSAGDIGVLIGIGFATRCVANLVITPRIHNVENLLPALRWLSFA
SLLFVGFHFFTGGSFLLMLLATVLFNLCCGPVIPLSDAMANYYSRLKMLDYGRTRLWGSIAFIAGSTVVGYLVAQFGTDM
ILYTALVGVLLSLVLAMRNPNVMPVTQSEKQAVRPKLGELLRESSVVKFLALMALLQGSHAAYYSFSAIYWKEAGHSEAI
IGYLWSLGVVAEVAVFALSKRLFSGWSMRTLFVVAAIGVMARWGITASTTAIFALVMVQMLHGVTFAMAHIAAIQYIQSE
EQNKMVALQALYNAIPLGAVIALMTTLSGWGYELWGANIFWGMAAMGALALFIKLDERSSVVEINQSGSEQLESNSKC

Sequences:

>Translated_398_residues
MFSPSPYGWISQYFLGFFFAYGVYLPFWALWFEDQGVSAGDIGVLIGIGFATRCVANLVITPRIHNVENLLPALRWLSFA
SLLFVGFHFFTGGSFLLMLLATVLFNLCCGPVIPLSDAMANYYSRLKMLDYGRTRLWGSIAFIAGSTVVGYLVAQFGTDM
ILYTALVGVLLSLVLAMRNPNVMPVTQSEKQAVRPKLGELLRESSVVKFLALMALLQGSHAAYYSFSAIYWKEAGHSEAI
IGYLWSLGVVAEVAVFALSKRLFSGWSMRTLFVVAAIGVMARWGITASTTAIFALVMVQMLHGVTFAMAHIAAIQYIQSE
EQNKMVALQALYNAIPLGAVIALMTTLSGWGYELWGANIFWGMAAMGALALFIKLDERSSVVEINQSGSEQLESNSKC
>Mature_398_residues
MFSPSPYGWISQYFLGFFFAYGVYLPFWALWFEDQGVSAGDIGVLIGIGFATRCVANLVITPRIHNVENLLPALRWLSFA
SLLFVGFHFFTGGSFLLMLLATVLFNLCCGPVIPLSDAMANYYSRLKMLDYGRTRLWGSIAFIAGSTVVGYLVAQFGTDM
ILYTALVGVLLSLVLAMRNPNVMPVTQSEKQAVRPKLGELLRESSVVKFLALMALLQGSHAAYYSFSAIYWKEAGHSEAI
IGYLWSLGVVAEVAVFALSKRLFSGWSMRTLFVVAAIGVMARWGITASTTAIFALVMVQMLHGVTFAMAHIAAIQYIQSE
EQNKMVALQALYNAIPLGAVIALMTTLSGWGYELWGANIFWGMAAMGALALFIKLDERSSVVEINQSGSEQLESNSKC

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=366, Percent_Identity=40.9836065573771, Blast_Score=272, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 43655; Mature: 43655

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHCCCCC
>Mature Secondary Structure
MFSPSPYGWISQYFLGFFFAYGVYLPFWALWFEDQGVSAGDIGVLIGIGFATRCVANLVI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
TPRIHNVENLLPALRWLSFASLLFVGFHFFTGGSFLLMLLATVLFNLCCGPVIPLSDAMA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NYYSRLKMLDYGRTRLWGSIAFIAGSTVVGYLVAQFGTDMILYTALVGVLLSLVLAMRNP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
NVMPVTQSEKQAVRPKLGELLRESSVVKFLALMALLQGSHAAYYSFSAIYWKEAGHSEAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHEECCCCCCHHH
IGYLWSLGVVAEVAVFALSKRLFSGWSMRTLFVVAAIGVMARWGITASTTAIFALVMVQM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LHGVTFAMAHIAAIQYIQSEEQNKMVALQALYNAIPLGAVIALMTTLSGWGYELWGANIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHH
WGMAAMGALALFIKLDERSSVVEINQSGSEQLESNSKC
HHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]