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The map label for this gene is trmL [H]

Identifier: 218710836

GI number: 218710836

Start: 3070342

End: 3070821

Strand: Direct

Name: trmL [H]

Synonym: VS_2919

Alternate gene names: 218710836

Gene position: 3070342-3070821 (Clockwise)

Preceding gene: 218710835

Following gene: 218710838

Centisome position: 93.06

GC content: 49.38

Gene sequence:

>480_bases
ATGTTTGATATCGCTCTATACGAACCTGAAATTGCGCCCAATACGGGTAACATCATCCGCCTATGTGCTAACTGCGGCGC
AAACCTGCACCTGATTGAGCCGCTTGGCTTTGATTTTGAAGAGAAGAAAGTTCGTCGTGCTGGCTTGGATTACCACGACC
TAGCGCGTGTTAAGCGTCACAAGAACCTAGAAGCCTTTCTTGAGTATCTAGAGAATGAACGCGAGGGTGACTTCCGCATC
TTTGCTTGCACGACAAAGACCACAGGTCACCACGTGGATGCGAAATTCCAACAAGGTGATGTGTTGATGTTTGGCCCTGA
GACACGTGGCCTGCCTGCAGAGTTCATCGAAAGCATGCCAATGGAACAACGCATTCGTATTCCAATGATGCCAGATGCGC
GTAGCCTCAACCTATCTAACGCGGTTGCTATTATTGCATTCGAAGCGTGGCGACAAATGGGCTTTGAAGGCGCGGTATAA

Upstream 100 bases:

>100_bases
AAGCACTCGCGAATAAAGCGTAAGCCAATGGTGGTGAACTGTACTCACTCAGCTTGCCACCGTATACTCTGGCTATCTTT
GATTATTCTTGTGAACCAAC

Downstream 100 bases:

>100_bases
CCGAAAGCTCCTCCCCCTGCTAGCAATAAAAAAGGCTCTTACCATTATCACTTTCTTGCGAAGGATAAAGGTAAAAGCCT
TTTCTGTTTTTCCCTCTATA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDFEEKKVRRAGLDYHDLARVKRHKNLEAFLEYLENEREGDFRI
FACTTKTTGHHVDAKFQQGDVLMFGPETRGLPAEFIESMPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAV

Sequences:

>Translated_159_residues
MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDFEEKKVRRAGLDYHDLARVKRHKNLEAFLEYLENEREGDFRI
FACTTKTTGHHVDAKFQQGDVLMFGPETRGLPAEFIESMPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAV
>Mature_159_residues
MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDFEEKKVRRAGLDYHDLARVKRHKNLEAFLEYLENEREGDFRI
FACTTKTTGHHVDAKFQQGDVLMFGPETRGLPAEFIESMPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=159, Percent_Identity=59.748427672956, Blast_Score=209, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18115; Mature: 18115

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDFEEKKVRRAGLDYHDLARVKRH
CCCEEEECCCCCCCCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHC
KNLEAFLEYLENEREGDFRIFACTTKTTGHHVDAKFQQGDVLMFGPETRGLPAEFIESMP
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEECCCCEEEECCCCCCCCHHHHHCCC
MEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAV
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCC
>Mature Secondary Structure
MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDFEEKKVRRAGLDYHDLARVKRH
CCCEEEECCCCCCCCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHC
KNLEAFLEYLENEREGDFRIFACTTKTTGHHVDAKFQQGDVLMFGPETRGLPAEFIESMP
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEECCCCEEEECCCCCCCCHHHHHCCC
MEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAV
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA