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The map label for this gene is pfkA [H]

Identifier: 218710830

GI number: 218710830

Start: 3064462

End: 3065430

Strand: Reverse

Name: pfkA [H]

Synonym: VS_2913

Alternate gene names: 218710830

Gene position: 3065430-3064462 (Counterclockwise)

Preceding gene: 218710831

Following gene: 218710827

Centisome position: 92.91

GC content: 47.27

Gene sequence:

>969_bases
GTGAGCATGATTAAGAAGATCGGTGTTTTGACCAGTGGTGGTGACGCTCCAGGCATGAACGCAGCAGTTCGCGGTGTAGT
TCGTACTGCGCTATCAGTTGGCATTGAAGTTTACGGCATTTACGACGGCTACCAAGGCCTTGTGGAAGACCGTATCGAAA
AGCTTGACCGTTCAAGCGTATCTGACGTGATCAACCGTGGTGGTACTTTCTTAGGTTCTGCACGTTTCCCTGAGTTCAAA
GACGTTGCTGTTCGTGAGAAAGGCATCGAGAACCTTAAGAAGCACGGTATCGAAGCACTTGTTGTTATCGGTGGTGACGG
TTCTTACATGGGTGCTAAGAAACTGACTGAGATGGGTTACCCATGTATCGGTCTTCCTGGCACAATCGACAATGATATCG
CGGGTACTGATTACACTATCGGTTACCTAACTGCGCTTAACACTGTTATCGATTCAATCGACCGTCTACGTGATACGTCT
TCTTCTCACCAACGTATTTCTATTGTTGAAATCATGGGCCGTCACTGTGGTGACCTTACGCTTATGTCAGCAATCGCGGG
TGGTTGTGAGTACATCATTACACCTGAGACTGGCCTAGATAAAGATCAGCTAATCAGCAATATTCAAGATGGTATTGCGA
AAGGCAAGAAGCACGCAATTATCGCTCTAACTGAGCTAATGATGGACGCGAACGAATTGGCTAAAGAGATTGAAACGGCA
ACAGGTCGTGAGACTCGCGCAACCGTTCTTGGTCACATTCAACGTGGTGGTCGTCCTACTGCATTTGACCGTGTTTTAGC
TTCTCGTATGGGCAACTACGCCGTTCACCTTCTTCAAGAAGGTCAAGGTGGTCGTTGTGTTGGTATCGAGAAAGAAGCTC
TTGTTCACCACGATATCATCGACTGTATCGAGAACATGCAGAACCCAGACCGTTCTGAGTTGTTCCGCGTTGCAGAAGAG
TTATTCTAA

Upstream 100 bases:

>100_bases
TTGAGGAAATGTAACATAACGTACAAATATAGATTTAAAATCTTGTTGATATACAATCAGCAAGAACAAGAATTGAATAA
TAGATTCCCAAAAATCGAGG

Downstream 100 bases:

>100_bases
GCCAAGCTTAGCGTTCGCTTAAAAAATATTAAAAACCGCTGATTGTTCAGCGGTTTTTTTGTGCCTGTCGTTTGAGTTTT
CACAAACGGCAGGCACAAAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSMIKKIGVLTSGGDAPGMNAAVRGVVRTALSVGIEVYGIYDGYQGLVEDRIEKLDRSSVSDVINRGGTFLGSARFPEFK
DVAVREKGIENLKKHGIEALVVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDSIDRLRDTS
SSHQRISIVEIMGRHCGDLTLMSAIAGGCEYIITPETGLDKDQLISNIQDGIAKGKKHAIIALTELMMDANELAKEIETA
TGRETRATVLGHIQRGGRPTAFDRVLASRMGNYAVHLLQEGQGGRCVGIEKEALVHHDIIDCIENMQNPDRSELFRVAEE
LF

Sequences:

>Translated_322_residues
MSMIKKIGVLTSGGDAPGMNAAVRGVVRTALSVGIEVYGIYDGYQGLVEDRIEKLDRSSVSDVINRGGTFLGSARFPEFK
DVAVREKGIENLKKHGIEALVVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDSIDRLRDTS
SSHQRISIVEIMGRHCGDLTLMSAIAGGCEYIITPETGLDKDQLISNIQDGIAKGKKHAIIALTELMMDANELAKEIETA
TGRETRATVLGHIQRGGRPTAFDRVLASRMGNYAVHLLQEGQGGRCVGIEKEALVHHDIIDCIENMQNPDRSELFRVAEE
LF
>Mature_321_residues
SMIKKIGVLTSGGDAPGMNAAVRGVVRTALSVGIEVYGIYDGYQGLVEDRIEKLDRSSVSDVINRGGTFLGSARFPEFKD
VAVREKGIENLKKHGIEALVVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDSIDRLRDTSS
SHQRISIVEIMGRHCGDLTLMSAIAGGCEYIITPETGLDKDQLISNIQDGIAKGKKHAIIALTELMMDANELAKEIETAT
GRETRATVLGHIQRGGRPTAFDRVLASRMGNYAVHLLQEGQGGRCVGIEKEALVHHDIIDCIENMQNPDRSELFRVAEEL
F

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=346, Percent_Identity=40.1734104046243, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI48762920, Length=313, Percent_Identity=42.1725239616613, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=41.025641025641, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=41.025641025641, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=41.025641025641, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=41.025641025641, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=71.5625, Blast_Score=479, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25147584, Length=341, Percent_Identity=37.8299120234604, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=38.5113268608414, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=32.7586206896552, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=313, Percent_Identity=39.9361022364217, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=315, Percent_Identity=35.8730158730159, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28573326, Length=341, Percent_Identity=39.0029325513196, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24652337, Length=341, Percent_Identity=39.2961876832845, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17647809, Length=341, Percent_Identity=39.2961876832845, Blast_Score=218, Evalue=5e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34797; Mature: 34666

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIKKIGVLTSGGDAPGMNAAVRGVVRTALSVGIEVYGIYDGYQGLVEDRIEKLDRSSV
CCHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHH
SDVINRGGTFLGSARFPEFKDVAVREKGIENLKKHGIEALVVIGGDGSYMGAKKLTEMGY
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCC
PCIGLPGTIDNDIAGTDYTIGYLTALNTVIDSIDRLRDTSSSHQRISIVEIMGRHCGDLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHH
LMSAIAGGCEYIITPETGLDKDQLISNIQDGIAKGKKHAIIALTELMMDANELAKEIETA
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGRETRATVLGHIQRGGRPTAFDRVLASRMGNYAVHLLQEGQGGRCVGIEKEALVHHDII
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEEECCCCCEEECCCHHHHHHHHHH
DCIENMQNPDRSELFRVAEELF
HHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SMIKKIGVLTSGGDAPGMNAAVRGVVRTALSVGIEVYGIYDGYQGLVEDRIEKLDRSSV
CHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHH
SDVINRGGTFLGSARFPEFKDVAVREKGIENLKKHGIEALVVIGGDGSYMGAKKLTEMGY
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCC
PCIGLPGTIDNDIAGTDYTIGYLTALNTVIDSIDRLRDTSSSHQRISIVEIMGRHCGDLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHH
LMSAIAGGCEYIITPETGLDKDQLISNIQDGIAKGKKHAIIALTELMMDANELAKEIETA
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGRETRATVLGHIQRGGRPTAFDRVLASRMGNYAVHLLQEGQGGRCVGIEKEALVHHDII
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEEECCCCCEEECCCHHHHHHHHHH
DCIENMQNPDRSELFRVAEELF
HHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]