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The map label for this gene is hslU

Identifier: 218710819

GI number: 218710819

Start: 3056366

End: 3057706

Strand: Direct

Name: hslU

Synonym: VS_2902

Alternate gene names: 218710819

Gene position: 3056366-3057706 (Clockwise)

Preceding gene: 218710818

Following gene: 218710820

Centisome position: 92.64

GC content: 47.35

Gene sequence:

>1341_bases
ATGTCTGAGATGACTCCTCGCGAAATTGTTCACGAACTCAATCGCCACATTATCGGCCAAGACAACGCTAAGCGCTCAGT
GGCTATCGCTCTGCGTAACCGCTGGCGTCGTATGCAGCTTGAAGAAAGTCTGCGTGTTGAAGTATCACCAAAAAACATCC
TGATGATCGGCCCAACCGGTGTCGGTAAAACTGAAATTGCTCGCCGTCTTGCGAAACTAGCAAACGCGCCTTTCATCAAG
GTAGAAGCGACTAAGTTCACTGAAGTGGGCTACGTAGGTAAAGAAGTTGAAACCATCATCCGTGATCTAACGGACGTTGC
GATTAAGATGACACATCAACAAGCGATGGAAAAAGTACAATACCGCGCTGAAGAACAAGCTGAAGAACGTATTCTTGATG
CCCTTCTACCACCAGCGCGTGATGCTTGGGGTCAGAACGAGCAATCAACTGAAGACACAACCTCTTCAAACACTCGCCAG
ATTTTCCGCAAGAAACTGCGTGAAGGTAAGCTAGACGACAAAGAGATTGAAGTTGATGTAGCGGCGCCACAAATGGGTGT
TGAAATCATGTCACCTCCAGGCATGGAAGAGATGACTAACCAGCTACAAGGCATGTTCCAAAATCTCGCTGGCGACACCA
AGAAAAAGCGTAAGATGAAAATCAAAGACGCATTCAAAGCACTAACAGAAGAAGAAGCTGCGAAGCTTGTGAACCAGGAA
GAGCTAAAAGAGAGCGCGATCTTCAACGCTGAAAACAACGGCATCGTATTCATCGATGAGATCGACAAAATCTGTAAGCG
TGGCGACAGCTCTGGCCCAGACGTATCTCGTGAAGGTGTTCAACGTGACCTGCTCCCTCTTATCGAAGGCAGCACAGTAT
CAACGAAGCACGGTATGGTTAAAACTGACCACATCTTGTTTATCACTTCAGGTGCATTCCAAGTGGCTAAGCCATCAGAC
CTAATCCCTGAACTGCAAGGTCGCCTGCCAATCCGTGTTGAACTTGAAGCACTATCAGCACATGATTTCAAACGTATTCT
TACTGAGCCAAAGGCATCACTGACAGAGCAGTACATTGCCCTGATGAAAACAGAAGATGTCGGCATTGAGTTCACAGAAG
ATGGCATCAACCAAATTGCTGATGCAGCATGGCGTGTGAACGAAACCACAGAAAACATCGGTGCGCGTCGTCTACACACA
GTTATGGAGCGCCTAATGGATGAGATTTCATTCGACGCAACAGACCGAGCAGGCAGCAAATTGGTGATTGATGAAGCTTA
CGTAATATCTAAGCTTGGCGAGCTCGTAGAAGACGAAGACCTAAGTCGCTTCATCCTGTAG

Upstream 100 bases:

>100_bases
GAAGAACTAGAAAGCACCGTTGAACTGCCAAAGCCAGAGTAACGACCGCTTCCATCAATAACGTTGTATTCAACAGTGAT
TAAAGAATTAAGGAAAAACC

Downstream 100 bases:

>100_bases
CACAGGAAACTCCAACTTATTGCTCTATCTCAAAGAAATAGCAGCTAATAAGCGAATTCAAGGCCCACTTTCGAGTGGGC
TTTTTATTACCCGAAAAATG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVSPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVETIIRDLTDVAIKMTHQQAMEKVQYRAEEQAEERILDALLPPARDAWGQNEQSTEDTTSSNTRQ
IFRKKLREGKLDDKEIEVDVAAPQMGVEIMSPPGMEEMTNQLQGMFQNLAGDTKKKRKMKIKDAFKALTEEEAAKLVNQE
ELKESAIFNAENNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFITSGAFQVAKPSD
LIPELQGRLPIRVELEALSAHDFKRILTEPKASLTEQYIALMKTEDVGIEFTEDGINQIADAAWRVNETTENIGARRLHT
VMERLMDEISFDATDRAGSKLVIDEAYVISKLGELVEDEDLSRFIL

Sequences:

>Translated_446_residues
MSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVSPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVETIIRDLTDVAIKMTHQQAMEKVQYRAEEQAEERILDALLPPARDAWGQNEQSTEDTTSSNTRQ
IFRKKLREGKLDDKEIEVDVAAPQMGVEIMSPPGMEEMTNQLQGMFQNLAGDTKKKRKMKIKDAFKALTEEEAAKLVNQE
ELKESAIFNAENNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFITSGAFQVAKPSD
LIPELQGRLPIRVELEALSAHDFKRILTEPKASLTEQYIALMKTEDVGIEFTEDGINQIADAAWRVNETTENIGARRLHT
VMERLMDEISFDATDRAGSKLVIDEAYVISKLGELVEDEDLSRFIL
>Mature_445_residues
SEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVSPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVETIIRDLTDVAIKMTHQQAMEKVQYRAEEQAEERILDALLPPARDAWGQNEQSTEDTTSSNTRQI
FRKKLREGKLDDKEIEVDVAAPQMGVEIMSPPGMEEMTNQLQGMFQNLAGDTKKKRKMKIKDAFKALTEEEAAKLVNQEE
LKESAIFNAENNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFITSGAFQVAKPSDL
IPELQGRLPIRVELEALSAHDFKRILTEPKASLTEQYIALMKTEDVGIEFTEDGINQIADAAWRVNETTENIGARRLHTV
MERLMDEISFDATDRAGSKLVIDEAYVISKLGELVEDEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=77.5784753363229, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=47, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=124, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_VIBSL (B7VLM7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418440.1
- ProteinModelPortal:   B7VLM7
- SMR:   B7VLM7
- GeneID:   7162386
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2902
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 50226; Mature: 50095

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 259-259 BINDING 324-324 BINDING 396-396

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVSPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVETIIRDLTDVAIKMTHQQAMEKVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAEEQAEERILDALLPPARDAWGQNEQSTEDTTSSNTRQIFRKKLREGKLDDKEIEVDV
HHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
AAPQMGVEIMSPPGMEEMTNQLQGMFQNLAGDTKKKRKMKIKDAFKALTEEEAAKLVNQE
ECCCCCCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ELKESAIFNAENNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGSTVSTKHGMV
HHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
KTDHILFITSGAFQVAKPSDLIPELQGRLPIRVELEALSAHDFKRILTEPKASLTEQYIA
EECEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHH
LMKTEDVGIEFTEDGINQIADAAWRVNETTENIGARRLHTVMERLMDEISFDATDRAGSK
HHHCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LVIDEAYVISKLGELVEDEDLSRFIL
EEEHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVSPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVETIIRDLTDVAIKMTHQQAMEKVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAEEQAEERILDALLPPARDAWGQNEQSTEDTTSSNTRQIFRKKLREGKLDDKEIEVDV
HHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
AAPQMGVEIMSPPGMEEMTNQLQGMFQNLAGDTKKKRKMKIKDAFKALTEEEAAKLVNQE
ECCCCCCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ELKESAIFNAENNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGSTVSTKHGMV
HHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
KTDHILFITSGAFQVAKPSDLIPELQGRLPIRVELEALSAHDFKRILTEPKASLTEQYIA
EECEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHH
LMKTEDVGIEFTEDGINQIADAAWRVNETTENIGARRLHTVMERLMDEISFDATDRAGSK
HHHCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LVIDEAYVISKLGELVEDEDLSRFIL
EEEHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA