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The map label for this gene is lpdA [H]

Identifier: 218710798

GI number: 218710798

Start: 3030352

End: 3031818

Strand: Direct

Name: lpdA [H]

Synonym: VS_2881

Alternate gene names: 218710798

Gene position: 3030352-3031818 (Clockwise)

Preceding gene: 218710797

Following gene: 218710801

Centisome position: 91.85

GC content: 48.33

Gene sequence:

>1467_bases
ATGAAACAAGTCAATGTAGATGTAGCAGTTATCGGGGGCGGTACGGCAGGTTTAGGTTCTTACCGCGCTGCAAAAGCACA
CACTGACAGTGTTGTGATGATTGAAGGCGGCCCTTACGGTACAACCTGTGCTCGTGTTGGTTGTATGCCATCTAAACTGC
TTATTGCAGCGGCTGAAAGTGTACACCAAATTGAGAAAGCTCCGGCTTTTGGCGTTCACCCACAAGGTGATATCGTGATT
AACGGCCGTGAAGTAATGGACCGAGTGAAGTTTGAACGTGACCGTTTTGTTGGTTTTGTTTTAGAAGGTGTTGATGAAAT
TCCAGAACAAGACAAGATCCCTGGCTACGCAAAGTTCTTAGATGACAACACGCTACAAATCGATGACCACACGGTTGTGA
CGGCTAAGCGTATTGTTATCGCAACAGGTTCTCGCCCTGCCTACCCTGCAGTTTGGAATGAACTTGGCGACCGTCTAATC
ATTAATGATGACGTATTCAGCTGGGATGATTTACCAGAATCAGTGGCGATATTTGGCCCGGGCGTTATTGGCCTAGAGCT
TGGTCAGTCACTGCACCGCTTGGGTGTAAAAACAAAACTGTTCGGTTTAGGTGGTCAAGTAGGCCCGGTAACCGACCCAG
AGATCATGGCTTACGCAGACAAAGCCTTCAATGAAGAATTCTACCTTGATGCAGACGTAAAAATCGAAAGCATGAAACGT
ATTACTACTGACTCGGGTGAAGATCGCGTCGAGATCCAGTTCATCAATAAAGAAGGTGAACTAGAAACTAACGTCGTTGA
ATACGTACTGGCAGCTACAGGTCGTCGTCCAAATACAGACAAGCTTGGCCTAGAGAATACCTCTCTAGAACTTGATGAAC
GTGGTGTGCCAGTTGCAGACCACTACACGCTACAAACATCGCTGCCATCAGTATTTATTGCGGGGGATGCAAGCAACCAA
CTGCCTCTACTGCATGAAGCAGCAGACCAAGCACGTATTGCGGGTGATAATGCAGGTCGCTTCCCTGAGATTCGTGCAGG
CCTACGCCGCTCTAAAATCTCTGCGGTATTCTCTGACCCACAAATCGCGATGGTTGGTGAAACTTACAAAGAGATCACGA
CACGCTTAGGTACATGTGGTTGTTTCGCAACAGGTGAAGTGTCTTTCGAGAACCAAGGTCGCTCACGAGTGATGCTGCGC
AACAAAGGTATTCTGCACGTATACGGCGAGCAAGGTACAGGCCGCTTCCTTGGTGCTGAAATGATGGGACCAAACGCAGA
ACACTTGGCTCACCTATTAGCTTGGGCACACCAGAACAAGATGACGGTTTCTGAAATGCTAGACATGCCGTTCTACCACC
CAGTGATCGAAGAAGGTGTACGAACAGCGCTGCGTGACCTCAATGCGAAACTGCACCTAGGTCCAGAGATGGTGAAACAC
TGTCTAGATTGTGGCCCTGGTTGTTAA

Upstream 100 bases:

>100_bases
AACAGAATCACAAAACTGACCCGCAATCCTGACTCTTAAAAAATCACCAGAGTTGGAGGGGTTCGTTCAAACTAAATTTA
GCTATTGCGAGAAAATTATT

Downstream 100 bases:

>100_bases
TCGCAGATCATCAGGAAAGATAGCAGCTTGCTCTATTAACAGAAACTAGATACTAAAAAGGAAAGCCATTGGCTTTCCTT
TTTGTTTTTGGCAACGAGTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPAFGVHPQGDIVI
NGREVMDRVKFERDRFVGFVLEGVDEIPEQDKIPGYAKFLDDNTLQIDDHTVVTAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFSWDDLPESVAIFGPGVIGLELGQSLHRLGVKTKLFGLGGQVGPVTDPEIMAYADKAFNEEFYLDADVKIESMKR
ITTDSGEDRVEIQFINKEGELETNVVEYVLAATGRRPNTDKLGLENTSLELDERGVPVADHYTLQTSLPSVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMVKH
CLDCGPGC

Sequences:

>Translated_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPAFGVHPQGDIVI
NGREVMDRVKFERDRFVGFVLEGVDEIPEQDKIPGYAKFLDDNTLQIDDHTVVTAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFSWDDLPESVAIFGPGVIGLELGQSLHRLGVKTKLFGLGGQVGPVTDPEIMAYADKAFNEEFYLDADVKIESMKR
ITTDSGEDRVEIQFINKEGELETNVVEYVLAATGRRPNTDKLGLENTSLELDERGVPVADHYTLQTSLPSVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMVKH
CLDCGPGC
>Mature_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPAFGVHPQGDIVI
NGREVMDRVKFERDRFVGFVLEGVDEIPEQDKIPGYAKFLDDNTLQIDDHTVVTAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFSWDDLPESVAIFGPGVIGLELGQSLHRLGVKTKLFGLGGQVGPVTDPEIMAYADKAFNEEFYLDADVKIESMKR
ITTDSGEDRVEIQFINKEGELETNVVEYVLAATGRRPNTDKLGLENTSLELDERGVPVADHYTLQTSLPSVFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMVKH
CLDCGPGC

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=27.2151898734177, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=24.4111349036403, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI22035672, Length=325, Percent_Identity=24, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=25.1585623678647, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87082354, Length=483, Percent_Identity=26.9151138716356, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789915, Length=370, Percent_Identity=28.3783783783784, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=22.9437229437229, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=26.5263157894737, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983419, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=491, Percent_Identity=26.8839103869654, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=387, Percent_Identity=24.5478036175711, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325240, Length=373, Percent_Identity=25.201072386059, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21358499, Length=480, Percent_Identity=26.0416666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640549, Length=501, Percent_Identity=23.9520958083832, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640553, Length=501, Percent_Identity=23.9520958083832, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=23.9520958083832, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53144; Mature: 53144

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPAFGVHPQGDIVINGREVMDRVKFERDRFVGFVLEGVDEIPEQDKIPGYAKFL
HHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
DDNTLQIDDHTVVTAKRIVIATGSRPAYPAVWNELGDRLIINDDVFSWDDLPESVAIFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEEECC
GVIGLELGQSLHRLGVKTKLFGLGGQVGPVTDPEIMAYADKAFNEEFYLDADVKIESMKR
CEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHH
ITTDSGEDRVEIQFINKEGELETNVVEYVLAATGRRPNTDKLGLENTSLELDERGVPVAD
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCC
HYTLQTSLPSVFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
CEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECHHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMVKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHH
CLDCGPGC
HHHCCCCC
>Mature Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPAFGVHPQGDIVINGREVMDRVKFERDRFVGFVLEGVDEIPEQDKIPGYAKFL
HHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
DDNTLQIDDHTVVTAKRIVIATGSRPAYPAVWNELGDRLIINDDVFSWDDLPESVAIFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEEECC
GVIGLELGQSLHRLGVKTKLFGLGGQVGPVTDPEIMAYADKAFNEEFYLDADVKIESMKR
CEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHH
ITTDSGEDRVEIQFINKEGELETNVVEYVLAATGRRPNTDKLGLENTSLELDERGVPVAD
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCC
HYTLQTSLPSVFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
CEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGETYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECHHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTVSEMLDMPFYHPVIEEGVRTALRDLNAKLHLGPEMVKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHH
CLDCGPGC
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]