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The map label for this gene is kefB [H]

Identifier: 218710765

GI number: 218710765

Start: 2991984

End: 2993783

Strand: Reverse

Name: kefB [H]

Synonym: VS_2848

Alternate gene names: 218710765

Gene position: 2993783-2991984 (Counterclockwise)

Preceding gene: 218710766

Following gene: 218710764

Centisome position: 90.74

GC content: 48.67

Gene sequence:

>1800_bases
ATGATGGCTCTGACTAACGATTTTCTACAAAGTAGCGTTATATTTTTAGCAGCTGCTGTAGTTGCGGTTCCTATCGCACA
GCGCGCTGGATTAGGGTCGGTACTCGGTTACTTATTAGCGGGTGTGGCGATTGGCCCATGGGGGCTTGGTTTAATCAGTG
ATGTAGAGGCGATTCTACACTTCTCCGAATTCGGGGTGGTACTGCTGCTCTTCTTGATTGGTCTCGAACTTAATCCGAAA
AAACTGTGGCAGATGCGAGCCCCCATTCTAGGACTCGGTGGCGCGCAAGTGCTGATCACCACTCTGATTATTACCGCCAT
CGCGTGTATGTTTGGTTTAACTTGGCAAACTAGCTTAGTCATCGGCATGGGTTTAGCTTTGTCTTCGACCGCTATTGCGT
TGCGTGTTATTGAAGAGCGAGAGCTTGGTGGCAAAGAAGCAGGGCAGTCAGGCTTTGCGGTATTACTTTTCCAAGATATT
GCCGTAATCCCTATGTTGGCGATGCTGCCTTTGCTTGCTGGCAATACGGGCGGCAGTTGGGCTGACATGTTGTGGATGCT
AGGCGGTGTGATTGGCTTGCTTGTCGGTGGTCACTTCTTGTTGAGACCACTGTTCCGATATGTAGTCATGAGCGGTGTGC
GTGAGTTGTTCACGGTAGCAGCGCTGTTATTGGTGATTGGTATTGCCGTCATCATGCAGCAGATTGGTTTGTCGATGGCA
TTAGGTACTTTCTTGGCGGGCGTACTTCTGGCTGAAAGTGAATATCGACACGAGTTAGAAATCGCGATTGACCCATTCAA
AGGGTTACTGCTCGGCCTGTTCTTTATCTCGGTTGGCATGGCGGTAAATCTAGGTTTATTGGCAGAAAGCCCATTCGCAA
TACTAATAGCTGTAATGGCCCTCGTGGTGTTGAAAGGTTTAGTGCTGTACGCGCTAGCTCGTATCTTTGGAACACAGGCA
AAAGCGCGTAGCCGAATGGCGATGATTCTCAGCCAAGGTGGTGAGTTTGCTTTCGTTATTTTTACCGCGGCGAGTGCGCA
GGGCATCTTAAGTGGCGAACAGGTGTCGTTCTTACTGGTGGTAGTGAGCCTGTCTATGGTGACCACGCCGCTGATGCTTA
AGCTGCAAGACCGATTCTTTGCTCGTCAGCTGAACCAAATCAGCGAAAGTGCGATGTCTTCGGATGTGGTCGATCGTAGC
CCTCGAGTGATTATTGCGGGCTTTGGTCGTTTCGGTCAGATCATTGGCCGCTTGATGTACGCCAACAAGATTCGTATCAC
GGTTCTTGAGAGTGATGCCAGCCAAATACACATTCTGAGAAAGTTCGGCTACAAGGTATTTTATGGTGATTCAACACACT
TAGAACTGTTACGTGCCGCAGGTGCAGACAAAGCGGAAGCGATCGTGTTGTGTACCGACTCTCCAGACGAAATCATGAAA
ACTGTCGACTTGTGTAAGCAGCATTTCCCACAGTTAAAGATTTTAGCGCGTGCTCGAAGCCGTGTTGAAGCGTATCAATT
ACTTAACCACGGTGTGAGCAACTACTCGCGTGAAACCTTCCTTGGGGCATTAGATTTAGGTCGTCAAACATTGACCGAAC
TTGGCATGCATCCATATAAAGCGAAGCGAGCAGAAGCACACTTTAGGAAACTGGATAATGGTATGCTGAAAGAGTTGCTG
CCTCAGCATAACGAAGATGCCGAGTTAGCCCAAAGAGCGAAAGAGGCTCGCAAAGAGCTTGAAGAGATTTTTGGACACGA
GATGGAAAACGATCACCAATCTCGAAACTATTGGCAGTAG

Upstream 100 bases:

>100_bases
GTTTTACACTGGGCACGAAATGTCACCGATATGACGCGTTACCAACACGCAGAAGCGTATCGAAATTGGTTGCGAGACCC
GCTACAAGATATAGGAGCGA

Downstream 100 bases:

>100_bases
CTTGAAGTGATAACTAATGCTTGAGCTTAGAAATGAAGCTTGGGCATAGACATATAGCTTGAGCTCTAGAAATAAAAGTC
TAGATAACTGATCTAATAAT

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MMALTNDFLQSSVIFLAAAVVAVPIAQRAGLGSVLGYLLAGVAIGPWGLGLISDVEAILHFSEFGVVLLLFLIGLELNPK
KLWQMRAPILGLGGAQVLITTLIITAIACMFGLTWQTSLVIGMGLALSSTAIALRVIEERELGGKEAGQSGFAVLLFQDI
AVIPMLAMLPLLAGNTGGSWADMLWMLGGVIGLLVGGHFLLRPLFRYVVMSGVRELFTVAALLLVIGIAVIMQQIGLSMA
LGTFLAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMAVNLGLLAESPFAILIAVMALVVLKGLVLYALARIFGTQA
KARSRMAMILSQGGEFAFVIFTAASAQGILSGEQVSFLLVVVSLSMVTTPLMLKLQDRFFARQLNQISESAMSSDVVDRS
PRVIIAGFGRFGQIIGRLMYANKIRITVLESDASQIHILRKFGYKVFYGDSTHLELLRAAGADKAEAIVLCTDSPDEIMK
TVDLCKQHFPQLKILARARSRVEAYQLLNHGVSNYSRETFLGALDLGRQTLTELGMHPYKAKRAEAHFRKLDNGMLKELL
PQHNEDAELAQRAKEARKELEEIFGHEMENDHQSRNYWQ

Sequences:

>Translated_599_residues
MMALTNDFLQSSVIFLAAAVVAVPIAQRAGLGSVLGYLLAGVAIGPWGLGLISDVEAILHFSEFGVVLLLFLIGLELNPK
KLWQMRAPILGLGGAQVLITTLIITAIACMFGLTWQTSLVIGMGLALSSTAIALRVIEERELGGKEAGQSGFAVLLFQDI
AVIPMLAMLPLLAGNTGGSWADMLWMLGGVIGLLVGGHFLLRPLFRYVVMSGVRELFTVAALLLVIGIAVIMQQIGLSMA
LGTFLAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMAVNLGLLAESPFAILIAVMALVVLKGLVLYALARIFGTQA
KARSRMAMILSQGGEFAFVIFTAASAQGILSGEQVSFLLVVVSLSMVTTPLMLKLQDRFFARQLNQISESAMSSDVVDRS
PRVIIAGFGRFGQIIGRLMYANKIRITVLESDASQIHILRKFGYKVFYGDSTHLELLRAAGADKAEAIVLCTDSPDEIMK
TVDLCKQHFPQLKILARARSRVEAYQLLNHGVSNYSRETFLGALDLGRQTLTELGMHPYKAKRAEAHFRKLDNGMLKELL
PQHNEDAELAQRAKEARKELEEIFGHEMENDHQSRNYWQ
>Mature_599_residues
MMALTNDFLQSSVIFLAAAVVAVPIAQRAGLGSVLGYLLAGVAIGPWGLGLISDVEAILHFSEFGVVLLLFLIGLELNPK
KLWQMRAPILGLGGAQVLITTLIITAIACMFGLTWQTSLVIGMGLALSSTAIALRVIEERELGGKEAGQSGFAVLLFQDI
AVIPMLAMLPLLAGNTGGSWADMLWMLGGVIGLLVGGHFLLRPLFRYVVMSGVRELFTVAALLLVIGIAVIMQQIGLSMA
LGTFLAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMAVNLGLLAESPFAILIAVMALVVLKGLVLYALARIFGTQA
KARSRMAMILSQGGEFAFVIFTAASAQGILSGEQVSFLLVVVSLSMVTTPLMLKLQDRFFARQLNQISESAMSSDVVDRS
PRVIIAGFGRFGQIIGRLMYANKIRITVLESDASQIHILRKFGYKVFYGDSTHLELLRAAGADKAEAIVLCTDSPDEIMK
TVDLCKQHFPQLKILARARSRVEAYQLLNHGVSNYSRETFLGALDLGRQTLTELGMHPYKAKRAEAHFRKLDNGMLKELL
PQHNEDAELAQRAKEARKELEEIFGHEMENDHQSRNYWQ

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=360, Percent_Identity=28.0555555555556, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1789749, Length=599, Percent_Identity=57.0951585976628, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=601, Percent_Identity=46.4226289517471, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1786685, Length=535, Percent_Identity=27.4766355140187, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65243; Mature: 65243

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMALTNDFLQSSVIFLAAAVVAVPIAQRAGLGSVLGYLLAGVAIGPWGLGLISDVEAILH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FSEFGVVLLLFLIGLELNPKKLWQMRAPILGLGGAQVLITTLIITAIACMFGLTWQTSLV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IGMGLALSSTAIALRVIEERELGGKEAGQSGFAVLLFQDIAVIPMLAMLPLLAGNTGGSW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCHH
ADMLWMLGGVIGLLVGGHFLLRPLFRYVVMSGVRELFTVAALLLVIGIAVIMQQIGLSMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFLAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMAVNLGLLAESPFAILIAVMA
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVVLKGLVLYALARIFGTQAKARSRMAMILSQGGEFAFVIFTAASAQGILSGEQVSFLLV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHH
VVSLSMVTTPLMLKLQDRFFARQLNQISESAMSSDVVDRSPRVIIAGFGRFGQIIGRLMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
ANKIRITVLESDASQIHILRKFGYKVFYGDSTHLELLRAAGADKAEAIVLCTDSPDEIMK
HCCEEEEEEECCCHHHHHHHHHCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCHHHHHH
TVDLCKQHFPQLKILARARSRVEAYQLLNHGVSNYSRETFLGALDLGRQTLTELGMHPYK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
AKRAEAHFRKLDNGMLKELLPQHNEDAELAQRAKEARKELEEIFGHEMENDHQSRNYWQ
HHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA