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The map label for this gene is cysN

Identifier: 218710705

GI number: 218710705

Start: 2951418

End: 2952848

Strand: Reverse

Name: cysN

Synonym: VS_2788

Alternate gene names: 218710705

Gene position: 2952848-2951418 (Counterclockwise)

Preceding gene: 218710706

Following gene: 218710704

Centisome position: 89.5

GC content: 47.73

Gene sequence:

>1431_bases
ATGAATAGTGCAGTAGAAGCCGAATTGGCTGAACTAGGGATTGAAGGTTATCTAAGTCAGCATCAGCATAAATCTATGCT
TAGATTTTTAACTTGTGGCTCGGTAGATGATGGTAAAAGTACGCTGATCGGTCGCTTGCTCCATGATACAAAACAGATTT
ATGAAGATCAGCTAGCAGCGGTTCACTCGGATAGCCAACGAGTGGGTACCACAGGTGAGAAGCCTGATTTGGCACTGCTT
GTTGATGGCTTGCAGGCTGAACGTGAGCAAGGCATCACGATCGATGTGGCTTACCGCTACTTCTCGACGCAAAAACGTAA
GTTCATTATTGCTGATACTCCAGGGCATGAGCAGTACACGCGCAACATGGCGACAGGCGCTTCAACGTGTGATCTGGCTG
TGATCTTGATTGATGCTCGTAAGGGCGTTCTGGATCAAACACGTCGTCACTCGTTTATCTCTAACCTGCTTGGTTTGAAG
CATTTCATTGTTGCTGTAAACAAGATGGATCTGGTGGATTACTCGCAAGATCGTTTTGAAGAGATTCGCGATCAGTATCT
AGAGTTTGCAGAAAACCTAGAAGGCGAAACTAACATTCAGATCCTGCCAGTATCGGCGCTTGAAGGCATCAACGTTGCTG
CACCAAGTAAAGAGCTTGCATGGTTCGAAGGCCCATCTCTGCTAGAAGTGCTAGAGAACGTCGATATCGATCAAAAGCGT
TCTGCAGGTGAATTCCGATTCCCAGTGCAGTATGTGAATCGTCCGAACTTAGACTTCCGTGGCTTTGCTGGCACCGTTGC
CTCTGGCCGTGTGAGTGTTGGTGATGAAATCAAGGCGCTGCCGTCAGGTAAAACGTCGAAAGTTGCACGCATTGTAACTT
TTGATGGTGATTTGGAGTCTGCACAAGCGGGCTTGGCGGTAACGCTAACCCTTGAAGATGAGATTGATATCAGCCGTGGT
GATTTAATTGTGCTAGAAAACGCGCAGATTGAATCCACTAACCACGTATTGGCTGACATCGTGTGGATGACAGAGCAACC
ATTGCAACCGGGCAAAGCTTACGACATCAAGATCGCAGGCAAGAAAACCGTTGGCCAGGTTGAAACAGTTCGTCACCAAT
ACGACATCAACAACCTATCGACTCACGCTGTCGATGAGTTGCCACTGAATGGCATTGGCTTGTGTGAATGGTCACTGAAC
GAGACTGTCGCGCTGGATAAATACCGTGAGAGTGCCGATACCGGTGGTTTCATCGTTATCGACCGACTAACCAACGTGAC
GGTTGGCGCGGGTTTGATTCGAGACCGTTTGGACTCTGTTGAACAGCAAGTCGGTAACTTCTCTGCATTTGAACTTGAGT
TCAACGCATTGGTACGCAAGCACTTCCCTCATTGGGATGCCAAAGATCTAAGCCAACTACTGAAGTCATAA

Upstream 100 bases:

>100_bases
GTCTAGTGAGCGTCAAGGTCGAGCGATTGACCATGATCAGTCGGGCTCTATGGAGCTGAAAAAGCGCCAAGGTTATTTCT
AAGAATCTAAGGAAAGAAAA

Downstream 100 bases:

>100_bases
ACGACCAAGTAACAGCTTATTGAATCAGGCGGAAACCGATTGTTTCCGCCATAAAGGACGGGTATATGTGGCAACAAGGA
TTTGTATTAGCGATTTTGCT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNSAVEAELAELGIEGYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDTKQIYEDQLAAVHSDSQRVGTTGEKPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEEIRDQYLEFAENLEGETNIQILPVSALEGINVAAPSKELAWFEGPSLLEVLENVDIDQKR
SAGEFRFPVQYVNRPNLDFRGFAGTVASGRVSVGDEIKALPSGKTSKVARIVTFDGDLESAQAGLAVTLTLEDEIDISRG
DLIVLENAQIESTNHVLADIVWMTEQPLQPGKAYDIKIAGKKTVGQVETVRHQYDINNLSTHAVDELPLNGIGLCEWSLN
ETVALDKYRESADTGGFIVIDRLTNVTVGAGLIRDRLDSVEQQVGNFSAFELEFNALVRKHFPHWDAKDLSQLLKS

Sequences:

>Translated_476_residues
MNSAVEAELAELGIEGYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDTKQIYEDQLAAVHSDSQRVGTTGEKPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEEIRDQYLEFAENLEGETNIQILPVSALEGINVAAPSKELAWFEGPSLLEVLENVDIDQKR
SAGEFRFPVQYVNRPNLDFRGFAGTVASGRVSVGDEIKALPSGKTSKVARIVTFDGDLESAQAGLAVTLTLEDEIDISRG
DLIVLENAQIESTNHVLADIVWMTEQPLQPGKAYDIKIAGKKTVGQVETVRHQYDINNLSTHAVDELPLNGIGLCEWSLN
ETVALDKYRESADTGGFIVIDRLTNVTVGAGLIRDRLDSVEQQVGNFSAFELEFNALVRKHFPHWDAKDLSQLLKS
>Mature_476_residues
MNSAVEAELAELGIEGYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDTKQIYEDQLAAVHSDSQRVGTTGEKPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEEIRDQYLEFAENLEGETNIQILPVSALEGINVAAPSKELAWFEGPSLLEVLENVDIDQKR
SAGEFRFPVQYVNRPNLDFRGFAGTVASGRVSVGDEIKALPSGKTSKVARIVTFDGDLESAQAGLAVTLTLEDEIDISRG
DLIVLENAQIESTNHVLADIVWMTEQPLQPGKAYDIKIAGKKTVGQVETVRHQYDINNLSTHAVDELPLNGIGLCEWSLN
ETVALDKYRESADTGGFIVIDRLTNVTVGAGLIRDRLDSVEQQVGNFSAFELEFNALVRKHFPHWDAKDLSQLLKS

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.0023201856148, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.0023201856148, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=324, Percent_Identity=29.6296296296296, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=29.6296296296296, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=29.6296296296296, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4503471, Length=275, Percent_Identity=32.3636363636364, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI4503475, Length=275, Percent_Identity=32, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=29.3515358361775, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI34147630, Length=348, Percent_Identity=28.448275862069, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI157426893, Length=379, Percent_Identity=22.4274406332454, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789108, Length=471, Percent_Identity=65.8174097664543, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=292, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789737, Length=141, Percent_Identity=29.7872340425532, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790412, Length=141, Percent_Identity=29.7872340425532, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532067, Length=311, Percent_Identity=31.5112540192926, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI115532065, Length=311, Percent_Identity=31.5112540192926, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=340, Percent_Identity=30.8823529411765, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17552884, Length=275, Percent_Identity=32.3636363636364, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17569207, Length=275, Percent_Identity=32.3636363636364, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=268, Percent_Identity=29.8507462686567, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.2281879194631, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=230, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=346, Percent_Identity=27.4566473988439, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=374, Percent_Identity=30.4812834224599, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6325337, Length=328, Percent_Identity=28.9634146341463, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319594, Length=328, Percent_Identity=28.9634146341463, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320377, Length=335, Percent_Identity=28.955223880597, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=234, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=214, Percent_Identity=28.9719626168224, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45553807, Length=275, Percent_Identity=32.7272727272727, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553816, Length=275, Percent_Identity=32.7272727272727, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651721, Length=275, Percent_Identity=32.7272727272727, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17864154, Length=275, Percent_Identity=32.7272727272727, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550900, Length=430, Percent_Identity=26.046511627907, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24652838, Length=275, Percent_Identity=32, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137572, Length=275, Percent_Identity=32, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=27.9883381924198, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281363316, Length=332, Percent_Identity=27.1084337349398, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17864358, Length=332, Percent_Identity=27.1084337349398, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921738, Length=320, Percent_Identity=28.75, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922690, Length=234, Percent_Identity=29.0598290598291, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_VIBSL (B7VKY2)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418326.1
- GeneID:   7162274
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2788
- HOGENOM:   HBG307581
- OMA:   SAQIYED
- ProtClustDB:   PRK05124
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 52730; Mature: 52730

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAVEAELAELGIEGYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDTKQIYEDQLAA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHSDSQRVGTTGEKPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFIVAVNKMDLVDYSQDRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EIRDQYLEFAENLEGETNIQILPVSALEGINVAAPSKELAWFEGPSLLEVLENVDIDQKR
HHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCC
SAGEFRFPVQYVNRPNLDFRGFAGTVASGRVSVGDEIKALPSGKTSKVARIVTFDGDLES
CCCCEECCHHHCCCCCCCCCCCCCCCCCCCEECCCCHHCCCCCCCCCEEEEEEECCCCCC
AQAGLAVTLTLEDEIDISRGDLIVLENAQIESTNHVLADIVWMTEQPLQPGKAYDIKIAG
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHEEECCCCCCCCCCEEEEEECC
KKTVGQVETVRHQYDINNLSTHAVDELPLNGIGLCEWSLNETVALDKYRESADTGGFIVI
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCEEEHHHHHHCCCCCCEEEE
DRLTNVTVGAGLIRDRLDSVEQQVGNFSAFELEFNALVRKHFPHWDAKDLSQLLKS
ECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNSAVEAELAELGIEGYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDTKQIYEDQLAA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHSDSQRVGTTGEKPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFIVAVNKMDLVDYSQDRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EIRDQYLEFAENLEGETNIQILPVSALEGINVAAPSKELAWFEGPSLLEVLENVDIDQKR
HHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCC
SAGEFRFPVQYVNRPNLDFRGFAGTVASGRVSVGDEIKALPSGKTSKVARIVTFDGDLES
CCCCEECCHHHCCCCCCCCCCCCCCCCCCCEECCCCHHCCCCCCCCCEEEEEEECCCCCC
AQAGLAVTLTLEDEIDISRGDLIVLENAQIESTNHVLADIVWMTEQPLQPGKAYDIKIAG
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHEEECCCCCCCCCCEEEEEECC
KKTVGQVETVRHQYDINNLSTHAVDELPLNGIGLCEWSLNETVALDKYRESADTGGFIVI
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCEEEHHHHHHCCCCCCEEEE
DRLTNVTVGAGLIRDRLDSVEQQVGNFSAFELEFNALVRKHFPHWDAKDLSQLLKS
ECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA