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The map label for this gene is mpl [H]

Identifier: 218710689

GI number: 218710689

Start: 2932502

End: 2933860

Strand: Reverse

Name: mpl [H]

Synonym: VS_2770

Alternate gene names: 218710689

Gene position: 2933860-2932502 (Counterclockwise)

Preceding gene: 218710691

Following gene: 218710688

Centisome position: 88.92

GC content: 47.24

Gene sequence:

>1359_bases
ATGCATATTCATATCTTAGGAATTTGTGGCACCTTCATGGGAGGTGCTGCGGTATTGGCTCGTCAATTAGGTCACAAGGT
TACCGGTAGTGACGCGAATGTTTACCCACCAATGAGCACTTTGTTAGAGTCTCAAGGGATTGAAATTATTGAAGGGTTTG
ACCCTAGCCAGTTAGAACCAAGGCCGGATCTCGTGGTGATTGGTAATGCGATGAGTCGTGGCAACCCGTGTGTTGAGTAT
GTATTGGATAACAACCTTAGATACACCTCAGGCCCGCAATGGTTGCAAGAGTTCTTGTTGCATGATCGTTGGGTATTAGC
GGTATCGGGAACGCATGGCAAGACAACGACATCGAGCATGCTGGCTTGGATTTTAGAAGACTGTGGCTATGCGCCTGGCT
TCTTAGTTGGCGGTGTGTTGGGTAACTTTGGTATCTCTGCTCGCCTTGGTGAAAGCATGTTCTTTGTGGTCGAAGCCGAC
GAATACGACAGTGCTTTTTTTGATAAACGATCTAAGTTCGTTCACTACCACCCAAGAACCTTGGTAATGAATAACCTCGA
GTTCGATCATGCTGACATATTCGATGATCTTGAGGCGATTAAGCGTCAGTTCCACCACTTAGTGCGCACTGTGCCAGGTA
ATGGCCGAATTTTCTCTCCTAAAGAAGATACGGCTATTCAAGATGTTTTATCTCGCGGATGCTGGAGTGAGACAGAATCG
AGTGGTGAACAAGGTGATTGGGATGCCAAGAAGCTGGTTAAAGACGGGTCTCAATTTGGAGTGTACTTCCAAGGCGAATG
CGTAGGAACTGTCGACTGGGATTTGGTTGGCGACCATAACGTCAATAATGCTTTGATGGCAATTGCAGCTGCGCGACATG
TAGGGGTGACACCTGATTTAGCGTGTGAATCGTTGGCGAAGTTCATCAACACCAAACGTCGTCTAGAGTTTAAAGGCGAA
GTGGCAGGCGTTTCTGTCTATGACGATTTTGCTCATCACCCAACGGCAATTGAACTTACGCTGGGCGGCTTACGTAATAA
GGTCGACACGAAAAAAATAATTGCCGTTTTAGAGCCTCGTTCTGCCACGATGAAACGTGGAGTGCACAAAGAAACGCTGG
CTGATTCGTTGATGCAAGCGGATTCTACTTACTTATTCCAACCCGATAACATTGATTGGTCGGTACAAGACGTCGCCGAT
GCGTGTCACCAACCCGCGCACGTGAGTGATGATATGGATGCATTCGTTGCTAAGATTGTCTCTGAGGCACAAGCGGGCGA
CCAAATCTTGGTAATGAGTAATGGTGGCTTTGGTGGTATTCACCAGAAACTGTTGGATGGATTGGCACTCAAAGGCTAA

Upstream 100 bases:

>100_bases
GTTTTGCATTGCTACTCGCTTTTAATCGCCATTTAATGATAATGAGTGCAGTAGTTTGATTTAGACATGGTTCATTTAGC
CAAGCGTTTGGAAGCAATTT

Downstream 100 bases:

>100_bases
CGCATCCACGACCTTATAATTTGAAGTAATTGAGAACATGACAAAACACGATAAAACGATAACTCTGGCTTTCACTGGCG
CATCCGGTGCGCCTTATGGC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelateligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MHIHILGICGTFMGGAAVLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLEPRPDLVVIGNAMSRGNPCVEY
VLDNNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCGYAPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDDLEAIKRQFHHLVRTVPGNGRIFSPKEDTAIQDVLSRGCWSETES
SGEQGDWDAKKLVKDGSQFGVYFQGECVGTVDWDLVGDHNVNNALMAIAAARHVGVTPDLACESLAKFINTKRRLEFKGE
VAGVSVYDDFAHHPTAIELTLGGLRNKVDTKKIIAVLEPRSATMKRGVHKETLADSLMQADSTYLFQPDNIDWSVQDVAD
ACHQPAHVSDDMDAFVAKIVSEAQAGDQILVMSNGGFGGIHQKLLDGLALKG

Sequences:

>Translated_452_residues
MHIHILGICGTFMGGAAVLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLEPRPDLVVIGNAMSRGNPCVEY
VLDNNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCGYAPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDDLEAIKRQFHHLVRTVPGNGRIFSPKEDTAIQDVLSRGCWSETES
SGEQGDWDAKKLVKDGSQFGVYFQGECVGTVDWDLVGDHNVNNALMAIAAARHVGVTPDLACESLAKFINTKRRLEFKGE
VAGVSVYDDFAHHPTAIELTLGGLRNKVDTKKIIAVLEPRSATMKRGVHKETLADSLMQADSTYLFQPDNIDWSVQDVAD
ACHQPAHVSDDMDAFVAKIVSEAQAGDQILVMSNGGFGGIHQKLLDGLALKG
>Mature_452_residues
MHIHILGICGTFMGGAAVLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLEPRPDLVVIGNAMSRGNPCVEY
VLDNNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCGYAPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDDLEAIKRQFHHLVRTVPGNGRIFSPKEDTAIQDVLSRGCWSETES
SGEQGDWDAKKLVKDGSQFGVYFQGECVGTVDWDLVGDHNVNNALMAIAAARHVGVTPDLACESLAKFINTKRRLEFKGE
VAGVSVYDDFAHHPTAIELTLGGLRNKVDTKKIIAVLEPRSATMKRGVHKETLADSLMQADSTYLFQPDNIDWSVQDVAD
ACHQPAHVSDDMDAFVAKIVSEAQAGDQILVMSNGGFGGIHQKLLDGLALKG

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=452, Percent_Identity=69.0265486725664, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=371, Percent_Identity=29.6495956873315, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49509; Mature: 49509

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGAAVLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLEP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
RPDLVVIGNAMSRGNPCVEYVLDNNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSM
CCCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDCGYAPGFLVGGVLGNFGISARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHCCCCCHHHHHHHHHCCCCCEECCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVMNNLEFDHADIFDDLEAIKRQFHHLVRTVPGNGRIFSPKEDTAIQDVLSRGCWSETES
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
SGEQGDWDAKKLVKDGSQFGVYFQGECVGTVDWDLVGDHNVNNALMAIAAARHVGVTPDL
CCCCCCHHHHHHHHCCCCCCEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
ACESLAKFINTKRRLEFKGEVAGVSVYDDFAHHPTAIELTLGGLRNKVDTKKIIAVLEPR
HHHHHHHHHHHHHCEEECCCEEEEEEEHHHCCCCCEEEEEECCCCCCCCHHHEEEEECCC
SATMKRGVHKETLADSLMQADSTYLFQPDNIDWSVQDVADACHQPAHVSDDMDAFVAKIV
HHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
SEAQAGDQILVMSNGGFGGIHQKLLDGLALKG
HHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGAAVLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLEP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
RPDLVVIGNAMSRGNPCVEYVLDNNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSM
CCCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDCGYAPGFLVGGVLGNFGISARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHCCCCCHHHHHHHHHCCCCCEECCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVMNNLEFDHADIFDDLEAIKRQFHHLVRTVPGNGRIFSPKEDTAIQDVLSRGCWSETES
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
SGEQGDWDAKKLVKDGSQFGVYFQGECVGTVDWDLVGDHNVNNALMAIAAARHVGVTPDL
CCCCCCHHHHHHHHCCCCCCEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
ACESLAKFINTKRRLEFKGEVAGVSVYDDFAHHPTAIELTLGGLRNKVDTKKIIAVLEPR
HHHHHHHHHHHHHCEEECCCEEEEEEEHHHCCCCCEEEEEECCCCCCCCHHHEEEEECCC
SATMKRGVHKETLADSLMQADSTYLFQPDNIDWSVQDVADACHQPAHVSDDMDAFVAKIV
HHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
SEAQAGDQILVMSNGGFGGIHQKLLDGLALKG
HHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]