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The map label for this gene is rpoE [H]

Identifier: 218710575

GI number: 218710575

Start: 2814553

End: 2815170

Strand: Reverse

Name: rpoE [H]

Synonym: VS_2625

Alternate gene names: 218710575

Gene position: 2815170-2814553 (Counterclockwise)

Preceding gene: 218710576

Following gene: 218710574

Centisome position: 85.33

GC content: 43.2

Gene sequence:

>618_bases
GTGAAGGTAAGGGTTATAACAATAGGAGTACCCGCTCGAATGAACGAGCAGCTAACCGATCAAGTGTTGATTGAGCGAGT
TCAGAGTGGAGATAAGCAGGCATTTAACTTATTAGTGGTTAAGTATCAAAACAAAGTTTGTAATCTTATCTCTCGATACG
TGAATAATTCCGGTGATGTACCTGATGTAGCACAAGAAGCTTTTATTAAAGCTTACCGCGCGATACCTAACTTTCGTGGT
GAGAGTGCTTTCTATACATGGTTGTACCGAATTGCCGTGAACACCGCTAAAAATCATATTGTTGCCCAGAGCCGCAGGCC
GCCAGCAACAGATGTCGATGCAGAAGATGCAGAATATTACGAAACAGGCAGCGCGTTAAAAGAAATATCGAACCCTGAGA
ACTTAACGCTGTCAAACGAATTGAAACAAGTTGTTTTTGGAGCGATTGAAGCGCTACCCGAAGACTTAAAAACTGCAATG
ACGTTGCGTGAGCTCGAAGGTTTGAGCTACGAAGAGATTGCAGAAGTAATGGATTGCCCTGTAGGAACCGTACGTTCGCG
TATTTTCCGAGCTCGTGAAGCGGTAGAAAAGAAAATTAAACCTCTTTTACAACGCTAG

Upstream 100 bases:

>100_bases
TTTAGGAAAGTTGGAACTTTCGGTATTTGGCTTAGTCACAATAGTGCTCTTGTAGACGGTGGTGTCAATACTACATTTCG
CATAATTAGTACCCATATCT

Downstream 100 bases:

>100_bases
AACTTGTAATAATTATGGTGAAAATAATGGCTGATAAAGAAAAGCTTTCGGCACTCATGGATGGTGAAACGATCGATAAA
GCTCTCATAGTAGATCTCGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKVRVITIGVPARMNEQLTDQVLIERVQSGDKQAFNLLVVKYQNKVCNLISRYVNNSGDVPDVAQEAFIKAYRAIPNFRG
ESAFYTWLYRIAVNTAKNHIVAQSRRPPATDVDAEDAEYYETGSALKEISNPENLTLSNELKQVVFGAIEALPEDLKTAM
TLRELEGLSYEEIAEVMDCPVGTVRSRIFRAREAVEKKIKPLLQR

Sequences:

>Translated_205_residues
MKVRVITIGVPARMNEQLTDQVLIERVQSGDKQAFNLLVVKYQNKVCNLISRYVNNSGDVPDVAQEAFIKAYRAIPNFRG
ESAFYTWLYRIAVNTAKNHIVAQSRRPPATDVDAEDAEYYETGSALKEISNPENLTLSNELKQVVFGAIEALPEDLKTAM
TLRELEGLSYEEIAEVMDCPVGTVRSRIFRAREAVEKKIKPLLQR
>Mature_205_residues
MKVRVITIGVPARMNEQLTDQVLIERVQSGDKQAFNLLVVKYQNKVCNLISRYVNNSGDVPDVAQEAFIKAYRAIPNFRG
ESAFYTWLYRIAVNTAKNHIVAQSRRPPATDVDAEDAEYYETGSALKEISNPENLTLSNELKQVVFGAIEALPEDLKTAM
TLRELEGLSYEEIAEVMDCPVGTVRSRIFRAREAVEKKIKPLLQR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=192, Percent_Identity=75, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23165; Mature: 23165

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVITIGVPARMNEQLTDQVLIERVQSGDKQAFNLLVVKYQNKVCNLISRYVNNSGDV
CEEEEEEECCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDVAQEAFIKAYRAIPNFRGESAFYTWLYRIAVNTAKNHIVAQSRRPPATDVDAEDAEYY
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHH
ETGSALKEISNPENLTLSNELKQVVFGAIEALPEDLKTAMTLRELEGLSYEEIAEVMDCP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
VGTVRSRIFRAREAVEKKIKPLLQR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVRVITIGVPARMNEQLTDQVLIERVQSGDKQAFNLLVVKYQNKVCNLISRYVNNSGDV
CEEEEEEECCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDVAQEAFIKAYRAIPNFRGESAFYTWLYRIAVNTAKNHIVAQSRRPPATDVDAEDAEYY
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHH
ETGSALKEISNPENLTLSNELKQVVFGAIEALPEDLKTAMTLRELEGLSYEEIAEVMDCP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
VGTVRSRIFRAREAVEKKIKPLLQR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]