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The map label for this gene is nplT [H]

Identifier: 218710544

GI number: 218710544

Start: 2779668

End: 2781506

Strand: Direct

Name: nplT [H]

Synonym: VS_2593

Alternate gene names: 218710544

Gene position: 2779668-2781506 (Clockwise)

Preceding gene: 218710543

Following gene: 218710545

Centisome position: 84.25

GC content: 46.17

Gene sequence:

>1839_bases
ATGATTTCCGCTATGGCTATTACAAATTCAGTTATCAACAAACCGATTACAAAAAGCTCGCTGACTCACTCAGCAAAAAG
TGCAGACAGCTACGCTTACAACAATGAAACGCTTCACCTTCGCTTACGCTGTGCAAAAGGCGAACTAGACAAGGTTTCAC
TTTGGATAGGCGACCCGTACCACTGGGCAGAAGGTGGCCTCGATGGTGGCAACCTAGGTGGCAGTGATGCACACGGTTGG
GTGGGTGGCAATGAAGTGACCATGATTCATGAAGGGGAAACCGAGTACCACGACCACTGGTTTGCAGAGTTTACTCCACC
GAAACGTCGTAGCCGCTATGGCTTTATTTTGTACGGAAAAGGCGGCGAAAAGATTCTATTTGGAGAAAAACGCTGCGCCG
ACATTTCAACGACTGAAAACGCAGAAATCGAGCTGAGCAACTTAAGTAATTTCTTCTGTTTTCCTTACATAAACCCTCGT
GACGTTTTAAAGACACCGACGTGGATAAAAGACACCATTTGGTACCAAATATTCCCAGAGCGCTTTGCTAATGGTCGCCC
TGAAACATCACCTGCCAACGTGCAACCTTGGGGAACCAGACCTGTATCCGACAACTTTATGGGTGGCGATTTATGGGGCG
TGATCGATAAGCTCGATTACCTGCAAGATCTTGGCGTGAATGGTTTGTATTTGTGCCCTATTTTCACAGCCAACGCCAAC
CATAAATACGACACTGTCGACTACTACAATGTAGACCCGCACTTTGGCGGCAATGAAGCTTTTAAAGCACTCGTCGATGA
AGCCCATAAACGCGGCATGAAAATTATGCTCGACGCGGTGTTCAACCACATTGGGTCTCAATCTCCACTATGGCTAGACG
TTGTGAATAACGGGGCAAAGTCCAAATACGCCGACTGGTTTTGGATCAATCAGTTCCCTGTATACCCAGATACGCCAAAA
GAAGACTGGGATTTCTGGAACTTAAACTACGAAACTTTCGCCAACGTCGTCGAAATGCCAAAGCTGAATACTGAAAATGA
AGAGTGCCGCGCATACTTATTAGACGTGGCGCGCCACTGGGTAGAAGAGTTCAACATTGATGGATGGCGTTTAGACGTAG
CCAACGAAGTGGACCACGCGTTTTGGCGTGACTTCCGTAAAGTCGTAAAAGATGCCAACCCAGACTGCTACATTCTTGGG
GAGATTTGGCACGAAGGTATGCCTTGGCTACGCGGCGACCAATACGATTCGTTGATGAATTACCCGCTGACACAAGCCAT
TACCGACTACTTTGGTCTTGGCGACGTTGATAAAGAGAGCTTTGTAAATGCAGTGAACGCGTCGTACATGGCATACCCAC
GCAACGTAAACGAAGCGATGTTTAACTTATTAGATAGCCATGACACCACTCGCATTATCTCTTTATGCCAGGGCGACAAA
CGTAAAGCTAAATTAGCCTATTTATTTATGTTCACTCAAGTCGGTGCTCCATGCATTTATTATGGTGGCGAAATTGGTAT
GGATGGCGGCCGCGGCATGGGCAGCGAAGACAACCGAAAATGCATGATATGGGAAGAATCAGAGCAAGACTTAGAATTCA
AAAACTTCATTCAAGAAATGATCGCTTTGCGTAAAGCCAATCCCGATTTTAATCAGCCTTGCATTGACTGGTTAAACGTG
GAGGATGAAAAAGGGACGGCAGACAAAGAGTGTATCGCTTACCGCCGTGGCAATTTAATGTTTGTTCTAAACAACAGCGA
TAAAGATAAACAGATCATGCTCGATGGAAAATCACTGACCATTAGCGCTTATGGCTACGTGATTGAGCAAGCGAACTAA

Upstream 100 bases:

>100_bases
ATTATTGGTTGCAGTGCCCATCACTTTGCTATTTGTTGCGACTCAAAAACACATTACTACCGGCCTCGTCAGCGGCGCAG
TTAAAGAATAATAATTAGGG

Downstream 100 bases:

>100_bases
ATCCAAGTGTGTCATTAATCAAATCATGCTCATTAAAGCAGAACCAAATATAGTGATGGGAATCGCGATTGATTCCCACT
CTATTTACTCGGTCGCCAGC

Product: Neopullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MISAMAITNSVINKPITKSSLTHSAKSADSYAYNNETLHLRLRCAKGELDKVSLWIGDPYHWAEGGLDGGNLGGSDAHGW
VGGNEVTMIHEGETEYHDHWFAEFTPPKRRSRYGFILYGKGGEKILFGEKRCADISTTENAEIELSNLSNFFCFPYINPR
DVLKTPTWIKDTIWYQIFPERFANGRPETSPANVQPWGTRPVSDNFMGGDLWGVIDKLDYLQDLGVNGLYLCPIFTANAN
HKYDTVDYYNVDPHFGGNEAFKALVDEAHKRGMKIMLDAVFNHIGSQSPLWLDVVNNGAKSKYADWFWINQFPVYPDTPK
EDWDFWNLNYETFANVVEMPKLNTENEECRAYLLDVARHWVEEFNIDGWRLDVANEVDHAFWRDFRKVVKDANPDCYILG
EIWHEGMPWLRGDQYDSLMNYPLTQAITDYFGLGDVDKESFVNAVNASYMAYPRNVNEAMFNLLDSHDTTRIISLCQGDK
RKAKLAYLFMFTQVGAPCIYYGGEIGMDGGRGMGSEDNRKCMIWEESEQDLEFKNFIQEMIALRKANPDFNQPCIDWLNV
EDEKGTADKECIAYRRGNLMFVLNNSDKDKQIMLDGKSLTISAYGYVIEQAN

Sequences:

>Translated_612_residues
MISAMAITNSVINKPITKSSLTHSAKSADSYAYNNETLHLRLRCAKGELDKVSLWIGDPYHWAEGGLDGGNLGGSDAHGW
VGGNEVTMIHEGETEYHDHWFAEFTPPKRRSRYGFILYGKGGEKILFGEKRCADISTTENAEIELSNLSNFFCFPYINPR
DVLKTPTWIKDTIWYQIFPERFANGRPETSPANVQPWGTRPVSDNFMGGDLWGVIDKLDYLQDLGVNGLYLCPIFTANAN
HKYDTVDYYNVDPHFGGNEAFKALVDEAHKRGMKIMLDAVFNHIGSQSPLWLDVVNNGAKSKYADWFWINQFPVYPDTPK
EDWDFWNLNYETFANVVEMPKLNTENEECRAYLLDVARHWVEEFNIDGWRLDVANEVDHAFWRDFRKVVKDANPDCYILG
EIWHEGMPWLRGDQYDSLMNYPLTQAITDYFGLGDVDKESFVNAVNASYMAYPRNVNEAMFNLLDSHDTTRIISLCQGDK
RKAKLAYLFMFTQVGAPCIYYGGEIGMDGGRGMGSEDNRKCMIWEESEQDLEFKNFIQEMIALRKANPDFNQPCIDWLNV
EDEKGTADKECIAYRRGNLMFVLNNSDKDKQIMLDGKSLTISAYGYVIEQAN
>Mature_612_residues
MISAMAITNSVINKPITKSSLTHSAKSADSYAYNNETLHLRLRCAKGELDKVSLWIGDPYHWAEGGLDGGNLGGSDAHGW
VGGNEVTMIHEGETEYHDHWFAEFTPPKRRSRYGFILYGKGGEKILFGEKRCADISTTENAEIELSNLSNFFCFPYINPR
DVLKTPTWIKDTIWYQIFPERFANGRPETSPANVQPWGTRPVSDNFMGGDLWGVIDKLDYLQDLGVNGLYLCPIFTANAN
HKYDTVDYYNVDPHFGGNEAFKALVDEAHKRGMKIMLDAVFNHIGSQSPLWLDVVNNGAKSKYADWFWINQFPVYPDTPK
EDWDFWNLNYETFANVVEMPKLNTENEECRAYLLDVARHWVEEFNIDGWRLDVANEVDHAFWRDFRKVVKDANPDCYILG
EIWHEGMPWLRGDQYDSLMNYPLTQAITDYFGLGDVDKESFVNAVNASYMAYPRNVNEAMFNLLDSHDTTRIISLCQGDK
RKAKLAYLFMFTQVGAPCIYYGGEIGMDGGRGMGSEDNRKCMIWEESEQDLEFKNFIQEMIALRKANPDFNQPCIDWLNV
EDEKGTADKECIAYRRGNLMFVLNNSDKDKQIMLDGKSLTISAYGYVIEQAN

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=396, Percent_Identity=23.2323232323232, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786604, Length=440, Percent_Identity=33.1818181818182, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1790687, Length=394, Percent_Identity=27.1573604060914, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789995, Length=474, Percent_Identity=26.1603375527426, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322245, Length=230, Percent_Identity=30, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321731, Length=225, Percent_Identity=28.8888888888889, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=225, Percent_Identity=28.8888888888889, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321726, Length=226, Percent_Identity=27.8761061946903, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=226, Percent_Identity=27.4336283185841, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6322021, Length=226, Percent_Identity=27.4336283185841, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324416, Length=226, Percent_Identity=27.4336283185841, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24586587, Length=230, Percent_Identity=27.3913043478261, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24586599, Length=225, Percent_Identity=28, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221330053, Length=226, Percent_Identity=27.4336283185841, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586589, Length=483, Percent_Identity=24.0165631469979, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586597, Length=223, Percent_Identity=27.8026905829596, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24586591, Length=230, Percent_Identity=29.1304347826087, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549022, Length=223, Percent_Identity=26.457399103139, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=26.6375545851528, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=26.6375545851528, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583745, Length=129, Percent_Identity=29.4573643410853, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586593, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 70037; Mature: 70037

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAMAITNSVINKPITKSSLTHSAKSADSYAYNNETLHLRLRCAKGELDKVSLWIGDPY
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEECCCEEEEEEEECCCCCEEEEEEECCCC
HWAEGGLDGGNLGGSDAHGWVGGNEVTMIHEGETEYHDHWFAEFTPPKRRSRYGFILYGK
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCHHCCCEEEEEECC
GGEKILFGEKRCADISTTENAEIELSNLSNFFCFPYINPRDVLKTPTWIKDTIWYQIFPE
CCCEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHCCCCHHHHHHHEEECHH
RFANGRPETSPANVQPWGTRPVSDNFMGGDLWGVIDKLDYLQDLGVNGLYLCPIFTANAN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
HKYDTVDYYNVDPHFGGNEAFKALVDEAHKRGMKIMLDAVFNHIGSQSPLWLDVVNNGAK
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
SKYADWFWINQFPVYPDTPKEDWDFWNLNYETFANVVEMPKLNTENEECRAYLLDVARHW
CCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VEEFNIDGWRLDVANEVDHAFWRDFRKVVKDANPDCYILGEIWHEGMPWLRGDQYDSLMN
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCHHHHHC
YPLTQAITDYFGLGDVDKESFVNAVNASYMAYPRNVNEAMFNLLDSHDTTRIISLCQGDK
CCHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCC
RKAKLAYLFMFTQVGAPCIYYGGEIGMDGGRGMGSEDNRKCMIWEESEQDLEFKNFIQEM
CHHEEEEEEEHHHHCCCEEEECCEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
IALRKANPDFNQPCIDWLNVEDEKGTADKECIAYRRGNLMFVLNNSDKDKQIMLDGKSLT
HHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHEECCCEEEEECCCCCCCEEEEECCEEE
ISAYGYVIEQAN
EEEEEEEEECCC
>Mature Secondary Structure
MISAMAITNSVINKPITKSSLTHSAKSADSYAYNNETLHLRLRCAKGELDKVSLWIGDPY
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEECCCEEEEEEEECCCCCEEEEEEECCCC
HWAEGGLDGGNLGGSDAHGWVGGNEVTMIHEGETEYHDHWFAEFTPPKRRSRYGFILYGK
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCHHCCCEEEEEECC
GGEKILFGEKRCADISTTENAEIELSNLSNFFCFPYINPRDVLKTPTWIKDTIWYQIFPE
CCCEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHCCCCHHHHHHHEEECHH
RFANGRPETSPANVQPWGTRPVSDNFMGGDLWGVIDKLDYLQDLGVNGLYLCPIFTANAN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
HKYDTVDYYNVDPHFGGNEAFKALVDEAHKRGMKIMLDAVFNHIGSQSPLWLDVVNNGAK
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
SKYADWFWINQFPVYPDTPKEDWDFWNLNYETFANVVEMPKLNTENEECRAYLLDVARHW
CCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VEEFNIDGWRLDVANEVDHAFWRDFRKVVKDANPDCYILGEIWHEGMPWLRGDQYDSLMN
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCHHHHHC
YPLTQAITDYFGLGDVDKESFVNAVNASYMAYPRNVNEAMFNLLDSHDTTRIISLCQGDK
CCHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCC
RKAKLAYLFMFTQVGAPCIYYGGEIGMDGGRGMGSEDNRKCMIWEESEQDLEFKNFIQEM
CHHEEEEEEEHHHHCCCEEEECCEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
IALRKANPDFNQPCIDWLNVEDEKGTADKECIAYRRGNLMFVLNNSDKDKQIMLDGKSLT
HHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHEECCCEEEEECCCCCCCEEEEECCEEE
ISAYGYVIEQAN
EEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]