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The map label for this gene is cycB [H]

Identifier: 218710541

GI number: 218710541

Start: 2776132

End: 2777376

Strand: Direct

Name: cycB [H]

Synonym: VS_2590

Alternate gene names: 218710541

Gene position: 2776132-2777376 (Clockwise)

Preceding gene: 218710522

Following gene: 218710542

Centisome position: 84.14

GC content: 44.98

Gene sequence:

>1245_bases
ATGAAAAAGAATCTATTCGCGGCAGCCTTAATGGCGGCAACGTTAACGACCAGTACATTAACGGCTGCAGCCGATGCAAA
CGCAGACACAATTCAGCCAGAAAATGGGGCTGAGTTGTTAATCTGGACCGACAAAACAACACTCGACTACATGGAGTACG
CCGCTCAGTCCTTTAATAAAGAGTTAGGATACGATATTGATTTTTCATTCCGTGGGTTAGCGCCGATCGATGCCGCTTCT
CGTTTGATTCAGGATGGCGGCTCTGCTCGCGTGGCAGATGTAGTCGAGATTGAACATGACTTATTGGGGCGATTAGTCGT
GGCAGGTGGGGCGATGGAGAATCTGGTATCTGCCGAACGTATCTCGAATACTTTTATGCCAAATGCGATTAATGCGTCTC
AAGCTGAAGGGGTGAGCTATGGCTTCCCAGTTAGCTTCGCCACTACCGCTCTGTTCTACAATAAAGATTTACTGACTAAA
GCACCTGAAACTTTTGAAGAAATTATTGAATTTTCAAAAACTTTCAACAATAAGCAAGAGCATAAATACGCACTGCTTTG
GGACATACAGAACTATTACGAGTCTCGTATGTTTGTCACGCTTTACGGCGCATACGAATTTGGTAATAACGGTACCGATG
CAAAAGACATCGGCATCAATTCTGAAAAAGCCCAACAAGGCTTAGAAGCAATGAAAACCCTGCAAGTGGCCAACAATTCA
AATCCAGTTGATATGCGCAACCCGCAAGTACGTCGTGGCTTATTTAATGAAGGAAAAGTCGCCGCTATTATCGACGGCCC
TTGGGCAATCGAAGGGTATCAAGAATCAGGGATAAATCTTGGTGTTGTACCTATGCCTAAATTAGCAGGCCAACAACCAC
GCACTTTTGCAACTGTTCGTCTTGCGGTTGTGTCTTCTTACACTGAGTATCCGAAAGCCGCACAATTATTTGCTGACTAC
CTTACTTCCGACGCGATGCTGAAAAAACGCTATGAGATGTCAAGAGCACTCCCTCCGATCCAGTCAGTTATGGAAGAGAT
CATTGTTGATGCCGATGAAGCCACTTACGCCATTATTACGCAAGGTTTCTACTCAGACGCAATGCCATCGATTCCTGAAA
TGGGCTTCATTTGGTCACCAATGGCCAGTGCTATTACAGACTACTGGGTAAACGATAAACCTGCGAGTTCTGTTCTTGAC
CGTGCGCTTTCGATCATCGAAGAACAAATAGAATTACAAGAGTAA

Upstream 100 bases:

>100_bases
GGTAATGTTGACATATTGTCGGCGAGACATAACATAGTAGTACTTAACGAACGACTACTATGATTTTAAATAGCGCGGTG
TCAGTATGAAAAATTCAGGA

Downstream 100 bases:

>100_bases
CCTCTGAACTCCCGTCGCGACTTACAAGCGACTTAGCTCATGCGCTGTGTCATAAAGCCACAGCGCTAGTTCAGGGCGTG
ACCAGAAAACGAATACATAA

Product: maltose/maltodextrin-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKKNLFAAALMAATLTTSTLTAAADANADTIQPENGAELLIWTDKTTLDYMEYAAQSFNKELGYDIDFSFRGLAPIDAAS
RLIQDGGSARVADVVEIEHDLLGRLVVAGGAMENLVSAERISNTFMPNAINASQAEGVSYGFPVSFATTALFYNKDLLTK
APETFEEIIEFSKTFNNKQEHKYALLWDIQNYYESRMFVTLYGAYEFGNNGTDAKDIGINSEKAQQGLEAMKTLQVANNS
NPVDMRNPQVRRGLFNEGKVAAIIDGPWAIEGYQESGINLGVVPMPKLAGQQPRTFATVRLAVVSSYTEYPKAAQLFADY
LTSDAMLKKRYEMSRALPPIQSVMEEIIVDADEATYAIITQGFYSDAMPSIPEMGFIWSPMASAITDYWVNDKPASSVLD
RALSIIEEQIELQE

Sequences:

>Translated_414_residues
MKKNLFAAALMAATLTTSTLTAAADANADTIQPENGAELLIWTDKTTLDYMEYAAQSFNKELGYDIDFSFRGLAPIDAAS
RLIQDGGSARVADVVEIEHDLLGRLVVAGGAMENLVSAERISNTFMPNAINASQAEGVSYGFPVSFATTALFYNKDLLTK
APETFEEIIEFSKTFNNKQEHKYALLWDIQNYYESRMFVTLYGAYEFGNNGTDAKDIGINSEKAQQGLEAMKTLQVANNS
NPVDMRNPQVRRGLFNEGKVAAIIDGPWAIEGYQESGINLGVVPMPKLAGQQPRTFATVRLAVVSSYTEYPKAAQLFADY
LTSDAMLKKRYEMSRALPPIQSVMEEIIVDADEATYAIITQGFYSDAMPSIPEMGFIWSPMASAITDYWVNDKPASSVLD
RALSIIEEQIELQE
>Mature_414_residues
MKKNLFAAALMAATLTTSTLTAAADANADTIQPENGAELLIWTDKTTLDYMEYAAQSFNKELGYDIDFSFRGLAPIDAAS
RLIQDGGSARVADVVEIEHDLLGRLVVAGGAMENLVSAERISNTFMPNAINASQAEGVSYGFPVSFATTALFYNKDLLTK
APETFEEIIEFSKTFNNKQEHKYALLWDIQNYYESRMFVTLYGAYEFGNNGTDAKDIGINSEKAQQGLEAMKTLQVANNS
NPVDMRNPQVRRGLFNEGKVAAIIDGPWAIEGYQESGINLGVVPMPKLAGQQPRTFATVRLAVVSSYTEYPKAAQLFADY
LTSDAMLKKRYEMSRALPPIQSVMEEIIVDADEATYAIITQGFYSDAMPSIPEMGFIWSPMASAITDYWVNDKPASSVLD
RALSIIEEQIELQE

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=415, Percent_Identity=24.8192771084337, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45629; Mature: 45629

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLFAAALMAATLTTSTLTAAADANADTIQPENGAELLIWTDKTTLDYMEYAAQSFNK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ELGYDIDFSFRGLAPIDAASRLIQDGGSARVADVVEIEHDLLGRLVVAGGAMENLVSAER
HCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISNTFMPNAINASQAEGVSYGFPVSFATTALFYNKDLLTKAPETFEEIIEFSKTFNNKQE
HHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCC
HKYALLWDIQNYYESRMFVTLYGAYEFGNNGTDAKDIGINSEKAQQGLEAMKTLQVANNS
CEEEEEEEHHHHHHCEEEEEEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCC
NPVDMRNPQVRRGLFNEGKVAAIIDGPWAIEGYQESGINLGVVPMPKLAGQQPRTFATVR
CCCCCCCCHHHHCCCCCCCEEEEECCCEEECCHHHCCCEECCCCCHHHCCCCCCHHHHHH
LAVVSSYTEYPKAAQLFADYLTSDAMLKKRYEMSRALPPIQSVMEEIIVDADEATYAIIT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEE
QGFYSDAMPSIPEMGFIWSPMASAITDYWVNDKPASSVLDRALSIIEEQIELQE
CCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKNLFAAALMAATLTTSTLTAAADANADTIQPENGAELLIWTDKTTLDYMEYAAQSFNK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ELGYDIDFSFRGLAPIDAASRLIQDGGSARVADVVEIEHDLLGRLVVAGGAMENLVSAER
HCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISNTFMPNAINASQAEGVSYGFPVSFATTALFYNKDLLTKAPETFEEIIEFSKTFNNKQE
HHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCC
HKYALLWDIQNYYESRMFVTLYGAYEFGNNGTDAKDIGINSEKAQQGLEAMKTLQVANNS
CEEEEEEEHHHHHHCEEEEEEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCC
NPVDMRNPQVRRGLFNEGKVAAIIDGPWAIEGYQESGINLGVVPMPKLAGQQPRTFATVR
CCCCCCCCHHHHCCCCCCCEEEEECCCEEECCHHHCCCEECCCCCHHHCCCCCCHHHHHH
LAVVSSYTEYPKAAQLFADYLTSDAMLKKRYEMSRALPPIQSVMEEIIVDADEATYAIIT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEE
QGFYSDAMPSIPEMGFIWSPMASAITDYWVNDKPASSVLDRALSIIEEQIELQE
CCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]