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The map label for this gene is acnB [H]

Identifier: 218710483

GI number: 218710483

Start: 2707261

End: 2709876

Strand: Reverse

Name: acnB [H]

Synonym: VS_2522

Alternate gene names: 218710483

Gene position: 2709876-2707261 (Counterclockwise)

Preceding gene: 218710484

Following gene: 218710482

Centisome position: 82.13

GC content: 46.48

Gene sequence:

>2616_bases
GTGAACCGCAATGAGAGGAAAAAAGTCGTGCTTGAAGCCTACCGTAAACACGTCGAAGAACGTGCTGCCGAGGGAGTTGT
CCCAAGACCACTAGATGCTGAGCAAGTCGCTGGCCTAGTTGAACTTCTAAAGAATCCACCTCAAGGTGAAGAAGAAATCA
TTCTTGACCTACTAGAAAATCGCATTCCACCGGGTGTAGATGAAGCTGCTTACGTAAAAGCTGGCTTCCTTACGGCTATC
ACTAAAGGTGAAGTAGCTTCTCCACTAGTAAGCAAAGCAAAAGCTGCACAACTGCTTGGTACTATGCAAGGTGGTTACAA
CATCGAGTCTCTAGTGTCTCTGTTAGACGATGCTGAGCTTGCTCCAATCGCAGTTAAAGCCCTGTCTCATACTCTACTGA
TGTTCGATGCGTTTTACGATGTAGAAGATAAAGCGAAAGCAGGTAATGCTTCAGCACAACAAGTACTTCAATCTTGGGCT
GATGCTGAATGGTTTACAGCGAAAAACAAAGTAGCGGAAAAGATCACCGTTAAAGTATTTAAAGTTACCGGTGAAACTAA
CACTGATGACTTATCACCAGCGCCTGATGCATGGTCACGTCCTGATATCCCAGTGCACGCGAAAGCGATGCTGAAGATGG
AACGTGATGGCATTACTCCTGATGAACAGGGTAGCGTTGGTCCAATCAATCAAATTGAAGAAATGCAAAAAGACGGCATT
CCACTGGCTTACGTTGGTGATGTTGTTGGTACAGGTTCTTCACGTAAATCGGCAACAAACTCTGTACTTTGGTTCATGGG
TGAAGATATCCCATTCGTACCAAACAAGCGTACTGGCGGTGTTTGTCTGGGTGGTAAAATTGCACCAATCTTCTACAACA
CAATGGAAGATTCAGGCGCACTACCCATTGAACTGAACGTACAAGACATGAACATGGGCGATGTCATTGATATCTACCCG
TACGAAGGTGTTGTTCACAAGAACGGTTCTGTGATTTCAAACTTTGAGCTAAGCAAAGTACTTCTTGATGAAGTGCGTGC
TGGTGGCCGTATTCCACTGATCATTGGTCGTGGCCTAACGGGTCGTGCTCGTGATGCTCTAGGCCTAGCTGAAACCGATC
TGTTTGCTAAACCAATCGATCCTTCTGCTTCTGATAAAGGCTACACATTAGCTCAGAAGATGGTAGGTAAAGCGTGTGGC
GTTGAAGGTGTGCGTGCGGGTCAGTACTGCGAACCTAAAATGACGACAGTGGGTTCTCAAGATACTACGGGTCCTATGAC
GCGTGATGAGCTGAAAGATCTGGCTTGTCTTGGCTTCTCTGCGGATCTTGTGATGCAGTCTTTCTGTCACACATCTGCAT
ACCCGAAACCAGTTGATGTAAACACTCACCATACACTGCCTGATTTCATCATGAACCGTGCAGGTGTTTCACTTCGTCCG
GGTGATGGTGTTATCCACTCATGGTTAAACCGTATGCTTCTTCCTGATACTGTAGGTACAGGTGGTGACTCGCATACTCG
TTTCCCTCTAGGTATTTCATTCCCTGCTGGTTCTGGTTTGGTAGCATTTGCTGCGGCAACAGGCGTTATGCCTCTTGATA
TGCCTGAATCTATCTTGGTTCGCTTTAAAGGCGAAATGCAACCGGGTATCACACTACGTGACCTAGTACATGCAATTCCG
CTTTACGGTATCAAGCAGGGTCTACTAACGGTAGAGAAAGCAGGAAAGATCAATGAGTTCTCTGGTCGTGTACTAGAAAT
TGAAGGTGTCGAGCACCTATCTGTTGAGCAAGCATTTGAACTTTCAGATGCATCTGCTGAGCGCTCTGCAGCGGGGTGTA
CGGTTAAGCTGTCTCAAGAGTCTATCGATGAGTACCTGAACTCGAATATCGTTATGCTTAAGTGGATGATTGCTGAAGGT
TATGGTGATGTTCGTACGATTGAGCGTCGTATCACGGCAATGGAAGAGTGGTTAGCGAACCCTGAGTTGCTATCTGCTGA
TTCAGATGCGGAATACGCGCACGTTATCGAGATTGATCTTGCTGACATCGATCAACCAATCCTATGTGCGCCAAACGATC
CAGATGATGCTCGTCTTCTTTCTGACGTTCAAGGCACTGAGATTCAAGAAGTGTTCATCGGTTCTTGTATGACCAACATC
GGTCACTTCCGTGCTGCAGGTAAGATGCTAGAAGAGTTTAATGGCTCTTTGAATACTCGCCTATGGGTTGCTCCGCCAAC
TAAGATGGATAAAGACCAACTGACAGAAGAAGGCTACTACGGCATCTTCGGTCGTGCTGGGGTTCGTATTGAAACTCCGG
GTTGTTCATTATGTATGGGTAACCAAGCGCGTGTTGCTGACGCTTCGACAGTAATGTCGACGTCTACTCGTAACTTCCCG
AACCGTTTGGGTAATGGTGCGAACGTTTATCTAGCTTCTGCTGAGCTTTCTGCAGTTGGTGCGATTCTTGGTCGTATCCC
AACTAAAGAAGAGTACTTAGCGTACGCTGAGAAGATCAATGCAACCGCTGCCGATACTTACCGTTACCTGAACTTCCATA
AAATGGGTCAGTACACGGACAAAGCAGACACAGTTATCTTCCAAGAACCAGCTTAG

Upstream 100 bases:

>100_bases
CCGCTCATCAATACAAATTGAATGACAAAAATGGCAATGGAGAGCCAAGTTTCCAAGGATGTTCGCTTCTTTATTTTACT
AATAATTATGAAGGTAAGAA

Downstream 100 bases:

>100_bases
TTCCTCGTTCTATTTGCAGCTGCAGCGTTGTTGACTGCGTACGTTCACCGGAGCGCCGGCCGCCCTAATCACATAGAGCT
TCTATGCTCATAGGGATGAA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 871; Mature: 871

Protein sequence:

>871_residues
MNRNERKKVVLEAYRKHVEERAAEGVVPRPLDAEQVAGLVELLKNPPQGEEEIILDLLENRIPPGVDEAAYVKAGFLTAI
TKGEVASPLVSKAKAAQLLGTMQGGYNIESLVSLLDDAELAPIAVKALSHTLLMFDAFYDVEDKAKAGNASAQQVLQSWA
DAEWFTAKNKVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPINQIEEMQKDGI
PLAYVGDVVGTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIYP
YEGVVHKNGSVISNFELSKVLLDEVRAGGRIPLIIGRGLTGRARDALGLAETDLFAKPIDPSASDKGYTLAQKMVGKACG
VEGVRAGQYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRP
GDGVIHSWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIP
LYGIKQGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIDEYLNSNIVMLKWMIAEG
YGDVRTIERRITAMEEWLANPELLSADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNI
GHFRAAGKMLEEFNGSLNTRLWVAPPTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADASTVMSTSTRNFP
NRLGNGANVYLASAELSAVGAILGRIPTKEEYLAYAEKINATAADTYRYLNFHKMGQYTDKADTVIFQEPA

Sequences:

>Translated_871_residues
MNRNERKKVVLEAYRKHVEERAAEGVVPRPLDAEQVAGLVELLKNPPQGEEEIILDLLENRIPPGVDEAAYVKAGFLTAI
TKGEVASPLVSKAKAAQLLGTMQGGYNIESLVSLLDDAELAPIAVKALSHTLLMFDAFYDVEDKAKAGNASAQQVLQSWA
DAEWFTAKNKVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPINQIEEMQKDGI
PLAYVGDVVGTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIYP
YEGVVHKNGSVISNFELSKVLLDEVRAGGRIPLIIGRGLTGRARDALGLAETDLFAKPIDPSASDKGYTLAQKMVGKACG
VEGVRAGQYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRP
GDGVIHSWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIP
LYGIKQGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIDEYLNSNIVMLKWMIAEG
YGDVRTIERRITAMEEWLANPELLSADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNI
GHFRAAGKMLEEFNGSLNTRLWVAPPTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADASTVMSTSTRNFP
NRLGNGANVYLASAELSAVGAILGRIPTKEEYLAYAEKINATAADTYRYLNFHKMGQYTDKADTVIFQEPA
>Mature_871_residues
MNRNERKKVVLEAYRKHVEERAAEGVVPRPLDAEQVAGLVELLKNPPQGEEEIILDLLENRIPPGVDEAAYVKAGFLTAI
TKGEVASPLVSKAKAAQLLGTMQGGYNIESLVSLLDDAELAPIAVKALSHTLLMFDAFYDVEDKAKAGNASAQQVLQSWA
DAEWFTAKNKVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPINQIEEMQKDGI
PLAYVGDVVGTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIYP
YEGVVHKNGSVISNFELSKVLLDEVRAGGRIPLIIGRGLTGRARDALGLAETDLFAKPIDPSASDKGYTLAQKMVGKACG
VEGVRAGQYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRP
GDGVIHSWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIP
LYGIKQGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIDEYLNSNIVMLKWMIAEG
YGDVRTIERRITAMEEWLANPELLSADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNI
GHFRAAGKMLEEFNGSLNTRLWVAPPTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADASTVMSTSTRNFP
NRLGNGANVYLASAELSAVGAILGRIPTKEEYLAYAEKINATAADTYRYLNFHKMGQYTDKADTVIFQEPA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=77.9582366589327, Blast_Score=1382, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=377, Percent_Identity=26.525198938992, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=380, Percent_Identity=26.0526315789474, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94299; Mature: 94299

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNERKKVVLEAYRKHVEERAAEGVVPRPLDAEQVAGLVELLKNPPQGEEEIILDLLEN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHC
RIPPGVDEAAYVKAGFLTAITKGEVASPLVSKAKAAQLLGTMQGGYNIESLVSLLDDAEL
CCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
APIAVKALSHTLLMFDAFYDVEDKAKAGNASAQQVLQSWADAEWFTAKNKVAEKITVKVF
CHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEE
KVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPINQIEEMQKDGI
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
PLAYVGDVVGTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGA
CEEEECHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCC
LPIELNVQDMNMGDVIDIYPYEGVVHKNGSVISNFELSKVLLDEVRAGGRIPLIIGRGLT
CEEEEEEEECCCCCEEEECCCCCEEECCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCC
GRARDALGLAETDLFAKPIDPSASDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQ
CCCHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRP
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEECC
GDGVIHSWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHE
RFKGEMQPGITLRDLVHAIPLYGIKQGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFE
EECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHH
LSDASAERSAAGCTVKLSQESIDEYLNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLAN
CCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
PELLSADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNI
CCEECCCCCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
GHFRAAGKMLEEFNGSLNTRLWVAPPTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMG
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCCCCEEEECCCCEEEECCCCCEEEC
NQARVADASTVMSTSTRNFPNRLGNGANVYLASAELSAVGAILGRIPTKEEYLAYAEKIN
CCCCCCCHHHHHHCCCCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHC
ATAADTYRYLNFHKMGQYTDKADTVIFQEPA
CCHHHHHHHHHHHHCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MNRNERKKVVLEAYRKHVEERAAEGVVPRPLDAEQVAGLVELLKNPPQGEEEIILDLLEN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHC
RIPPGVDEAAYVKAGFLTAITKGEVASPLVSKAKAAQLLGTMQGGYNIESLVSLLDDAEL
CCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
APIAVKALSHTLLMFDAFYDVEDKAKAGNASAQQVLQSWADAEWFTAKNKVAEKITVKVF
CHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEE
KVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPINQIEEMQKDGI
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
PLAYVGDVVGTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGA
CEEEECHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCC
LPIELNVQDMNMGDVIDIYPYEGVVHKNGSVISNFELSKVLLDEVRAGGRIPLIIGRGLT
CEEEEEEEECCCCCEEEECCCCCEEECCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCC
GRARDALGLAETDLFAKPIDPSASDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQ
CCCHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRP
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEECC
GDGVIHSWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHE
RFKGEMQPGITLRDLVHAIPLYGIKQGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFE
EECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHH
LSDASAERSAAGCTVKLSQESIDEYLNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLAN
CCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
PELLSADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNI
CCEECCCCCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
GHFRAAGKMLEEFNGSLNTRLWVAPPTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMG
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCCCCEEEECCCCEEEECCCCCEEEC
NQARVADASTVMSTSTRNFPNRLGNGANVYLASAELSAVGAILGRIPTKEEYLAYAEKIN
CCCCCCCHHHHHHCCCCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHC
ATAADTYRYLNFHKMGQYTDKADTVIFQEPA
CCHHHHHHHHHHHHCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]