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The map label for this gene is truB

Identifier: 218710443

GI number: 218710443

Start: 2652051

End: 2653004

Strand: Reverse

Name: truB

Synonym: VS_2480

Alternate gene names: 218710443

Gene position: 2653004-2652051 (Counterclockwise)

Preceding gene: 218710444

Following gene: 218710442

Centisome position: 80.41

GC content: 47.17

Gene sequence:

>954_bases
ATGGCTCGCCGTCGTAAAGGTCGCCCTATCAACGGGGTAATTCTGTTAGATAAGCCAACGGGCATTTCGTCTAATGATGC
ACTGCAAAAAGTAAAACGTATTTACTTTGCAGAGAAGGCAGGGCACACCGGTGCTCTTGATCCTCTTGCGACTGGCATGC
TGCCAATTTGTCTTGGTGAAGCAACGAAGTTTTCTCAGTTTCTATTGGATTCTGACAAGCGCTACGTAGTAATCGCTAAG
CTTGGTGAGCGTACCAATACCTCTGACTCTGATGGTGAAGTGGTAGAGACACGAGATGTTAACGTGACTCAAGAGCAACT
TGAACGCTGCATTGCAAGCTTCAAAGGGGAAACCGACCAGATTCCATCAATGTTCTCAGCATTGAAGTATCAAGGTAAGC
CTTTGTATGAATACGCACGTGCAGGTATCGAGGTGCCTCGCGAATCTCGTAAGATCACTGTTTACTCTATTGAACTACTT
CGCTTTGAAGGTGATGAAGTTGAGATGGAAGTGCATTGTTCAAAAGGCACTTACATCCGCACAATTACCGACGATCTTGG
TGAAATGCTAGGTTGTGGTGCCCACGTGACCATGCTTCGTCGTACTGGTGTTGCTAAGTATCCGTACGATCGTATGGTGA
CTTTGGAGCAGTTGAACGAGATCCTAGAGCAAGCGCAGGCGCAAGAAATTGCACCCAAAGAGCTGCTTGACCCACTATTA
ATGCCAATGGACACAGCCGTTGAAGACTTACCAGAAGTTAACTTAAACGCGGAACTGACTGACCTAGTTCAGCACGGTAT
GCCCGTTCAGGTTTCTGGTGCGCCAACTGAAGGTACGGTTCGCATGACAAGTGGTGAAGAGAAACTGTTTGTTGGTGTTG
CTCAGATTGCTGAAGATGGCCGAGTTGCACCGAAGCGTTTGGTTGTTTTCAGAGATGAAGAGCCACAAGCATAA

Upstream 100 bases:

>100_bases
TGCGTATGTCTAACTTAGTAAGTGAAGTTTTAAACGACGATAAGCGTAAGCAAGAAGAGTCTGGCCGTACTGACGAAACT
CAGTCTAAGGGCGAAGAGTA

Downstream 100 bases:

>100_bases
CGCTGAAACCTAGTTAGGTATTGGCGCTTAGCCAGGAAAAGCGAATCAATAATATAGAAAGCGAGCCCTTAATTTTAATG
GTTCGCTTTTTTCGTTTTTC

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARRRKGRPINGVILLDKPTGISSNDALQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYVVIAK
LGERTNTSDSDGEVVETRDVNVTQEQLERCIASFKGETDQIPSMFSALKYQGKPLYEYARAGIEVPRESRKITVYSIELL
RFEGDEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTMLRRTGVAKYPYDRMVTLEQLNEILEQAQAQEIAPKELLDPLL
MPMDTAVEDLPEVNLNAELTDLVQHGMPVQVSGAPTEGTVRMTSGEEKLFVGVAQIAEDGRVAPKRLVVFRDEEPQA

Sequences:

>Translated_317_residues
MARRRKGRPINGVILLDKPTGISSNDALQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYVVIAK
LGERTNTSDSDGEVVETRDVNVTQEQLERCIASFKGETDQIPSMFSALKYQGKPLYEYARAGIEVPRESRKITVYSIELL
RFEGDEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTMLRRTGVAKYPYDRMVTLEQLNEILEQAQAQEIAPKELLDPLL
MPMDTAVEDLPEVNLNAELTDLVQHGMPVQVSGAPTEGTVRMTSGEEKLFVGVAQIAEDGRVAPKRLVVFRDEEPQA
>Mature_316_residues
ARRRKGRPINGVILLDKPTGISSNDALQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYVVIAKL
GERTNTSDSDGEVVETRDVNVTQEQLERCIASFKGETDQIPSMFSALKYQGKPLYEYARAGIEVPRESRKITVYSIELLR
FEGDEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTMLRRTGVAKYPYDRMVTLEQLNEILEQAQAQEIAPKELLDPLLM
PMDTAVEDLPEVNLNAELTDLVQHGMPVQVSGAPTEGTVRMTSGEEKLFVGVAQIAEDGRVAPKRLVVFRDEEPQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=251, Percent_Identity=32.6693227091633, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI215599015, Length=210, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4503337, Length=210, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=67.0967741935484, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17553978, Length=236, Percent_Identity=30.0847457627119, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=325, Percent_Identity=27.6923076923077, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364187, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364185, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364183, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62471759, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17975520, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_VIBSL (B7VJH5)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418064.1
- GeneID:   7162017
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2480
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35177; Mature: 35046

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRKGRPINGVILLDKPTGISSNDALQKVKRIYFAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYVVIAKLGERTNTSDSDGEVVETRDVNVTQEQLERCIASFKGETDQ
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCH
IPSMFSALKYQGKPLYEYARAGIEVPRESRKITVYSIELLRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVTMLRRTGVAKYPYDRMVTLEQLNEILEQAQAQEIAPKELLDPLL
EHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MPMDTAVEDLPEVNLNAELTDLVQHGMPVQVSGAPTEGTVRMTSGEEKLFVGVAQIAEDG
CCHHHHHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCEEEEECCCCEEEEEHHHHHCCC
RVAPKRLVVFRDEEPQA
CCCCEEEEEEECCCCCC
>Mature Secondary Structure 
ARRRKGRPINGVILLDKPTGISSNDALQKVKRIYFAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYVVIAKLGERTNTSDSDGEVVETRDVNVTQEQLERCIASFKGETDQ
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCH
IPSMFSALKYQGKPLYEYARAGIEVPRESRKITVYSIELLRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVTMLRRTGVAKYPYDRMVTLEQLNEILEQAQAQEIAPKELLDPLL
EHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MPMDTAVEDLPEVNLNAELTDLVQHGMPVQVSGAPTEGTVRMTSGEEKLFVGVAQIAEDG
CCHHHHHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCEEEEECCCCEEEEEHHHHHCCC
RVAPKRLVVFRDEEPQA
CCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA