Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 218710430

GI number: 218710430

Start: 2637790

End: 2639370

Strand: Reverse

Name: prfC [H]

Synonym: VS_2467

Alternate gene names: 218710430

Gene position: 2639370-2637790 (Counterclockwise)

Preceding gene: 218710431

Following gene: 218710429

Centisome position: 80.0

GC content: 46.43

Gene sequence:

>1581_bases
ATGTCTTTCCAACAAGAAGTGAGCAAACGTAGAACGTTTGCGATTATCTCTCACCCGGATGCGGGTAAAACCACGATTAC
TGAAAAAGTTCTTTTATTCGGAAACGCGATTCAAAAAGCGGGTACCGTAAAAGGCCGTGGCTCTAACCAGCACGCTAAAT
CTGACTGGATGGAGATGGAAAAAGAACGTGGTATCTCGGTAACTACGTCTGTAATGCAATTCCCATACAACGATTGTCTA
GTCAACCTACTCGATACTCCTGGACACGAAGATTTCTCGGAAGATACGTACCGTACATTAACCGCGGTTGACTCTTGTTT
GATGGTTATCGATGCTGCGAAAGGTGTCGAGGATCGTACTCGTAAACTGATGGAAGTAACGCGTCTACGTGATACACCAA
TCGTAACGTTTATGAACAAACTTGACCGTGATGTTCGTGACCCAATGGAAGTGCTAGATGAAGTGGAAAGCGAGCTAGGT
ATGGCTTGTGCTCCAATCTCTTGGCCTATCGGTTGTGGTAAAGAGTTTAAAGGCGTTTACCACATTCACCGTGATGAAAC
GATCTTGTATGAATCTGGCCACGGCCATGAGATCCAAGAAGTTCGCATTATCAAAGGTCTAGATAACCCTGAACTAGACG
AAGCTGTTGGCGAAGATCTGGCGAGCAGCGTTCGTGAAGAGCTTGAATTGGTTATTGGCGCATGTCCTGAGTTTGATCAC
GAACTATTCCTTGCTGGCGAGTTGACTCCAGTTTACTTCGGTACTGCATTGGGTAACTTCGGTGTTGACCATATGCTTGA
TGGCCTAACTCGATGGGCTCCTGCGCCTCAAACACGTCAAGCAAATGAGCGTGATGTTGAAGCGACTGAAGAGAAGTTCT
CTGGTTTCGTATTTAAGATCCAAGCAAACATGGATCCAAAACACCGTGACCGTATCGCATTCATGCGTATCGTATCGGGT
ACTTACGACCAAGGTATGAAGATGAACCATGTTCGTACTGGTAAGAACGTCAGTATCTCTGATGCAGTAACCTTCATGGC
GGGTGACCGTGCTCGTGCTGAAAAAGCATACGCGGGTGACATAATTGGTCTGCATAACCACGGCACAATTCAGATTGGCG
ATACCTTTACTCAAGGCGAAAGCTTGAAGTTCGCAGGTATTCCTAACTTTGCGCCTGAGCTATTCCGTCGTATTCGTCTG
CGCGATCCATTGAAGCAGAAGCAACTTCTAAAAGGCTTGGTTCAGCTTTCTGAAGAGGGCGCGGTTCAAGTATTCCGTCC
TTTGCAAAATAACGATCTGATCGTTGGTGCAGTTGGTGTACTTCAGTTTGATGTGGTTGTAGCGCGCTTGAAAGCGGAAT
ACAACGTTGAAGCGATTTACGAAGGTGTGAACGTTGCAACTGCACGTTGGGTTGAGTGTGATGACGCTAAGAAACTAGAA
GAGTTCAAACGTAAGAGCCAAGCTAACCTAGCGTTAGATGGTGGTGATAACCTATCTTACATCGCACCGACTATGGTGAA
TTTGAACCTAGCTTCTGAACGTTTCCCAGAAGTTCAGTTCCGAGCGACGCGCGAGCACTAA

Upstream 100 bases:

>100_bases
TTTAGAAAGTGACACGCTTTCTGTATAATCCGCACACAGAATTCAAGGGCAAGTATTATCTACTTGCCCTTTTTACGCTT
TAGATCAGCAAAGGGCGACT

Downstream 100 bases:

>100_bases
TCACTCTGCGCTCTATTCGAGTTAAACAAAAACGTCTATTGTTCTGCAATAGGCGTTTTTTTATTCGTGTCATTTGAGGT
TAGAGGCTTAAGGTAAGTAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSFQQEVSKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPYNDCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRIIKGLDNPELDEAVGEDLASSVREELELVIGACPEFDH
ELFLAGELTPVYFGTALGNFGVDHMLDGLTRWAPAPQTRQANERDVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYDQGMKMNHVRTGKNVSISDAVTFMAGDRARAEKAYAGDIIGLHNHGTIQIGDTFTQGESLKFAGIPNFAPELFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVNVATARWVECDDAKKLE
EFKRKSQANLALDGGDNLSYIAPTMVNLNLASERFPEVQFRATREH

Sequences:

>Translated_526_residues
MSFQQEVSKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPYNDCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRIIKGLDNPELDEAVGEDLASSVREELELVIGACPEFDH
ELFLAGELTPVYFGTALGNFGVDHMLDGLTRWAPAPQTRQANERDVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYDQGMKMNHVRTGKNVSISDAVTFMAGDRARAEKAYAGDIIGLHNHGTIQIGDTFTQGESLKFAGIPNFAPELFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVNVATARWVECDDAKKLE
EFKRKSQANLALDGGDNLSYIAPTMVNLNLASERFPEVQFRATREH
>Mature_525_residues
SFQQEVSKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPYNDCLV
NLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDVRDPMEVLDEVESELGM
ACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRIIKGLDNPELDEAVGEDLASSVREELELVIGACPEFDHE
LFLAGELTPVYFGTALGNFGVDHMLDGLTRWAPAPQTRQANERDVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSGT
YDQGMKMNHVRTGKNVSISDAVTFMAGDRARAEKAYAGDIIGLHNHGTIQIGDTFTQGESLKFAGIPNFAPELFRRIRLR
DPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVNVATARWVECDDAKKLEE
FKRKSQANLALDGGDNLSYIAPTMVNLNLASERFPEVQFRATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=525, Percent_Identity=27.047619047619, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.6666666666667, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.6802030456853, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.7261410788382, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=30.8139534883721, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=29.2517006802721, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310132016, Length=126, Percent_Identity=29.3650793650794, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI310110807, Length=126, Percent_Identity=29.3650793650794, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI310123363, Length=126, Percent_Identity=29.3650793650794, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=82.4427480916031, Blast_Score=925, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=500, Percent_Identity=26.8, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=518, Percent_Identity=25.0965250965251, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=32.9608938547486, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=31.3432835820896, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=29.3233082706767, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=29.3233082706767, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=26.7223382045929, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=423, Percent_Identity=26.7139479905437, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.8278145695364, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.2151898734177, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.2151898734177, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=26.9709543568465, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221458488, Length=515, Percent_Identity=26.0194174757282, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58871; Mature: 58739

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQQEVSKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKLDRDVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYESGHGHEIQEVRIIKGLDNPELDEAVGEDLASSVREELELVIGACPEFDH
EEECCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELFLAGELTPVYFGTALGNFGVDHMLDGLTRWAPAPQTRQANERDVEATEEKFSGFVFKI
CEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEE
QANMDPKHRDRIAFMRIVSGTYDQGMKMNHVRTGKNVSISDAVTFMAGDRARAEKAYAGD
ECCCCCCHHHHHHHHHHHCCCHHCCCEECEECCCCCCCHHHHEEEEECCHHHHHHHHCCC
IIGLHNHGTIQIGDTFTQGESLKFAGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVNVATARWVECDDAKKLE
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCHHHHH
EFKRKSQANLALDGGDNLSYIAPTMVNLNLASERFPEVQFRATREH
HHHHHCCCCEEEECCCCCCEECCEEEEEECCHHCCCCCEEEECCCC
>Mature Secondary Structure 
SFQQEVSKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKLDRDVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYESGHGHEIQEVRIIKGLDNPELDEAVGEDLASSVREELELVIGACPEFDH
EEECCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELFLAGELTPVYFGTALGNFGVDHMLDGLTRWAPAPQTRQANERDVEATEEKFSGFVFKI
CEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEE
QANMDPKHRDRIAFMRIVSGTYDQGMKMNHVRTGKNVSISDAVTFMAGDRARAEKAYAGD
ECCCCCCHHHHHHHHHHHCCCHHCCCEECEECCCCCCCHHHHEEEEECCHHHHHHHHCCC
IIGLHNHGTIQIGDTFTQGESLKFAGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVNVATARWVECDDAKKLE
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCHHHHH
EFKRKSQANLALDGGDNLSYIAPTMVNLNLASERFPEVQFRATREH
HHHHHCCCCEEEECCCCCCEECCEEEEEECCHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA