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The map label for this gene is map [H]

Identifier: 218710319

GI number: 218710319

Start: 2522938

End: 2523816

Strand: Direct

Name: map [H]

Synonym: VS_2354

Alternate gene names: 218710319

Gene position: 2522938-2523816 (Clockwise)

Preceding gene: 218710297

Following gene: 218710320

Centisome position: 76.47

GC content: 44.6

Gene sequence:

>879_bases
ATGGCTGTAAAAATTAAAACTGCTGAAGAAATTGAAAAAATGCGCGTCGCCGGCAAGCTGGCTTCTGAAATTTTAGAGAT
GATTGAACCTCACATCCAAGTGGGTACAACGACAGAAGAGCTTAACCAAATCTGTCACGAGTACGCTCTAGAGAGAGGCG
CATACTCAGCACCACTTGATTACCACGGTTTCCCTAAGTCTATCTGTACGTCTATCAACCACATCGTGTGTCATGGTATT
CCAGCATCACAAGATGAGACTGGTAGCACTGGTCAATTCAAACCTGCAGTATTGAAAGATGGCGATATTCTAAACGTTGA
TATCACTGTGATTGTTCCTGATGACGAAAATGCAGACCTAAGCACTCGTCCGCAGGGTTACCATGGTGACACATCTAAAA
TGTTCCTTGTGGGTGAAGTTTCACCAGCAAACAAACGTCTATGCATGGTTGCTCAAGAAGCACTTTATGAAGGCATGCGT
CAGGTTAAACCAGGCGTTCAACTTGGCCAAGTTGGTACTGCTATCGAGAAGTACATCAAGACAAACAACAAGAATAACCC
ACGCGCTAAGTTCTCTATTGTAAAAGATTACTGTGGTCACGGCATTGGTTCTGAGTTCCACGAAGATCCACAAGTCGTTC
ACTACAAAAACAGCGATCGCACAGTGCTAAAAGCAGGCATGTGCTTCACTATCGAGCCAATGATCAATGCGGGTAAGTTT
GGCTGTCGTCTAGATGACGAAGATAGCTGGACAGTGTACACAGCTGACAGCAAGAACTCGGCTCAGTGGGAACACACATT
AGTTGTGACTGATACAGGTTGTGAAGTACTAACACTACGCAGTGATGATACGATTCCACGTATCATGAAGAACGCTTAG

Upstream 100 bases:

>100_bases
ACCAGCATTGTATGGATATGGGAATGTTGCGCTAAAATGCTGCACTGATAGAATAGCCCCAATATGCACACTTAGGTGCT
ACCAAATTAAGAGATATGCA

Downstream 100 bases:

>100_bases
TTCAACAAACTGAACACCTCAGACTTTTGAAAATATCCTCGCATTGCCGAGGATATTTTTTTATCCGCTCAATTTCGATT
TTGCATCAGCTTCTGTTAAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAVKIKTAEEIEKMRVAGKLASEILEMIEPHIQVGTTTEELNQICHEYALERGAYSAPLDYHGFPKSICTSINHIVCHGI
PASQDETGSTGQFKPAVLKDGDILNVDITVIVPDDENADLSTRPQGYHGDTSKMFLVGEVSPANKRLCMVAQEALYEGMR
QVKPGVQLGQVGTAIEKYIKTNNKNNPRAKFSIVKDYCGHGIGSEFHEDPQVVHYKNSDRTVLKAGMCFTIEPMINAGKF
GCRLDDEDSWTVYTADSKNSAQWEHTLVVTDTGCEVLTLRSDDTIPRIMKNA

Sequences:

>Translated_292_residues
MAVKIKTAEEIEKMRVAGKLASEILEMIEPHIQVGTTTEELNQICHEYALERGAYSAPLDYHGFPKSICTSINHIVCHGI
PASQDETGSTGQFKPAVLKDGDILNVDITVIVPDDENADLSTRPQGYHGDTSKMFLVGEVSPANKRLCMVAQEALYEGMR
QVKPGVQLGQVGTAIEKYIKTNNKNNPRAKFSIVKDYCGHGIGSEFHEDPQVVHYKNSDRTVLKAGMCFTIEPMINAGKF
GCRLDDEDSWTVYTADSKNSAQWEHTLVVTDTGCEVLTLRSDDTIPRIMKNA
>Mature_291_residues
AVKIKTAEEIEKMRVAGKLASEILEMIEPHIQVGTTTEELNQICHEYALERGAYSAPLDYHGFPKSICTSINHIVCHGIP
ASQDETGSTGQFKPAVLKDGDILNVDITVIVPDDENADLSTRPQGYHGDTSKMFLVGEVSPANKRLCMVAQEALYEGMRQ
VKPGVQLGQVGTAIEKYIKTNNKNNPRAKFSIVKDYCGHGIGSEFHEDPQVVHYKNSDRTVLKAGMCFTIEPMINAGKFG
CRLDDEDSWTVYTADSKNSAQWEHTLVVTDTGCEVLTLRSDDTIPRIMKNA

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=291, Percent_Identity=41.9243986254296, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI40385867, Length=277, Percent_Identity=35.7400722021661, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1786364, Length=293, Percent_Identity=52.2184300341297, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71996291, Length=291, Percent_Identity=43.298969072165, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323273, Length=281, Percent_Identity=40.2135231316726, Blast_Score=205, Evalue=9e-54,
Organism=Drosophila melanogaster, GI21355531, Length=282, Percent_Identity=42.5531914893617, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24583427, Length=276, Percent_Identity=38.0434782608696, Blast_Score=191, Evalue=3e-49,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 32249; Mature: 32118

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKIKTAEEIEKMRVAGKLASEILEMIEPHIQVGTTTEELNQICHEYALERGAYSAPLD
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCC
YHGFPKSICTSINHIVCHGIPASQDETGSTGQFKPAVLKDGDILNVDITVIVPDDENADL
CCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEEECCCCCCC
STRPQGYHGDTSKMFLVGEVSPANKRLCMVAQEALYEGMRQVKPGVQLGQVGTAIEKYIK
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TNNKNNPRAKFSIVKDYCGHGIGSEFHEDPQVVHYKNSDRTVLKAGMCFTIEPMINAGKF
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCC
GCRLDDEDSWTVYTADSKNSAQWEHTLVVTDTGCEVLTLRSDDTIPRIMKNA
CCEECCCCCEEEEEECCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
AVKIKTAEEIEKMRVAGKLASEILEMIEPHIQVGTTTEELNQICHEYALERGAYSAPLD
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCC
YHGFPKSICTSINHIVCHGIPASQDETGSTGQFKPAVLKDGDILNVDITVIVPDDENADL
CCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEEECCCCCCC
STRPQGYHGDTSKMFLVGEVSPANKRLCMVAQEALYEGMRQVKPGVQLGQVGTAIEKYIK
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TNNKNNPRAKFSIVKDYCGHGIGSEFHEDPQVVHYKNSDRTVLKAGMCFTIEPMINAGKF
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCC
GCRLDDEDSWTVYTADSKNSAQWEHTLVVTDTGCEVLTLRSDDTIPRIMKNA
CCEECCCCCEEEEEECCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]