Click here to switch to the map view.

The map label for this gene is yafJ [H]

Identifier: 218710286

GI number: 218710286

Start: 2486971

End: 2487816

Strand: Reverse

Name: yafJ [H]

Synonym: VS_2320

Alternate gene names: 218710286

Gene position: 2487816-2486971 (Counterclockwise)

Preceding gene: 218710287

Following gene: 218710283

Centisome position: 75.4

GC content: 45.27

Gene sequence:

>846_bases
ATGTGTGAATTGCTCGGTATGAGCGCGAATGTGCCAACTGATATTTGTTTTAGCTTTACTGGCCTTATGCAGCGTGGAGG
CAACACTGGTCCACATCGTGATGGCTGGGGTATTACTTTCTATGAGGGAAAGGGTTTTCGTACATTTAAAGACCCTAATC
CTAGTTGTGAATCTAAGATCGCTGAGCTGGTTCAAAACTACCCAATTAAGAGCCAAGCTGTTGTCAGTCATATCCGTCAA
GCCAATCGTGGTGGTGTTAATCTAGAAAATACTCACCCTTTTACTCGTGAGCTTTGGGGACGATACTGGACCTTTGCTCA
CAATGGTCAACTAACGGATTATGATGATCTGATTAGTGGTCGATTCAGACCTGTCGGTGAGACGGACAGCGAACTCTCTT
TCTGCTGGCTGTTGAAACAACTTGAAGACCGTTTTCCTGAACCTCCTCAAGATATGGAAGGTATGTTTCGCTTTGTCGCT
GAGTGCTGTGACCAGTTACGAGAGAAAGGTGTTTTCAATATGTTGCTCAGTGACGGTGAGTACGTGATGAACTATTGTAC
GAACCATTTATACTGGATAACAAGACGCGCTCCTTTTGGTAACGCGAGCCTGATTGATGAAGATGTTGAGATTAACTTCC
AAGAAGAAACTACGCCCAACGATGTAGTAACCGTAGTTGCAACTCAGCCTCTAACAGACAATGAAGAGTGGTTTAGAATG
AAGCCAGGTGAATACGCGCTGTTTCATTTTGGTGAGCTGATCGGCAACAACCACCAAGCGTTGGAAGATGTCGCTTATGC
TCCGAAGAAGGTGGCAAGCCAAGCGCCTACTGAACCATTGAGCTAA

Upstream 100 bases:

>100_bases
GTGATTCAGCTTGTTGAAAAAGAAATGGAATAATAGTTTTACAGACATTTGGAGCCTAGGTTTGTGGCTCCATTGGTTTA
AATAGGGAGTAGATTAAACC

Downstream 100 bases:

>100_bases
TCTAGTCGATACTATTATTTGTCCTTATTAAAAAAGGCCGCTTACTGTACTAATAAGTCAGTGAGCGGCCTTTTTGTTTT
CTGTTTTTAAGGCTTATTCT

Product: glutamine amidotransferases class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MCELLGMSANVPTDICFSFTGLMQRGGNTGPHRDGWGITFYEGKGFRTFKDPNPSCESKIAELVQNYPIKSQAVVSHIRQ
ANRGGVNLENTHPFTRELWGRYWTFAHNGQLTDYDDLISGRFRPVGETDSELSFCWLLKQLEDRFPEPPQDMEGMFRFVA
ECCDQLREKGVFNMLLSDGEYVMNYCTNHLYWITRRAPFGNASLIDEDVEINFQEETTPNDVVTVVATQPLTDNEEWFRM
KPGEYALFHFGELIGNNHQALEDVAYAPKKVASQAPTEPLS

Sequences:

>Translated_281_residues
MCELLGMSANVPTDICFSFTGLMQRGGNTGPHRDGWGITFYEGKGFRTFKDPNPSCESKIAELVQNYPIKSQAVVSHIRQ
ANRGGVNLENTHPFTRELWGRYWTFAHNGQLTDYDDLISGRFRPVGETDSELSFCWLLKQLEDRFPEPPQDMEGMFRFVA
ECCDQLREKGVFNMLLSDGEYVMNYCTNHLYWITRRAPFGNASLIDEDVEINFQEETTPNDVVTVVATQPLTDNEEWFRM
KPGEYALFHFGELIGNNHQALEDVAYAPKKVASQAPTEPLS
>Mature_281_residues
MCELLGMSANVPTDICFSFTGLMQRGGNTGPHRDGWGITFYEGKGFRTFKDPNPSCESKIAELVQNYPIKSQAVVSHIRQ
ANRGGVNLENTHPFTRELWGRYWTFAHNGQLTDYDDLISGRFRPVGETDSELSFCWLLKQLEDRFPEPPQDMEGMFRFVA
ECCDQLREKGVFNMLLSDGEYVMNYCTNHLYWITRRAPFGNASLIDEDVEINFQEETTPNDVVTVVATQPLTDNEEWFRM
KPGEYALFHFGELIGNNHQALEDVAYAPKKVASQAPTEPLS

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=71.6535433070866, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 32030; Mature: 32030

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLMQRGGNTGPHRDGWGITFYEGKGFRTFKDPNPSCESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AELVQNYPIKSQAVVSHIRQANRGGVNLENTHPFTRELWGRYWTFAHNGQLTDYDDLISG
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHCC
RFRPVGETDSELSFCWLLKQLEDRFPEPPQDMEGMFRFVAECCDQLREKGVFNMLLSDGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCH
YVMNYCTNHLYWITRRAPFGNASLIDEDVEINFQEETTPNDVVTVVATQPLTDNEEWFRM
HHHHHHCCCEEEEEECCCCCCCCEECCCCEEEECCCCCCCCEEEEEEECCCCCCCHHEEE
KPGEYALFHFGELIGNNHQALEDVAYAPKKVASQAPTEPLS
CCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLMQRGGNTGPHRDGWGITFYEGKGFRTFKDPNPSCESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AELVQNYPIKSQAVVSHIRQANRGGVNLENTHPFTRELWGRYWTFAHNGQLTDYDDLISG
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHCC
RFRPVGETDSELSFCWLLKQLEDRFPEPPQDMEGMFRFVAECCDQLREKGVFNMLLSDGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCH
YVMNYCTNHLYWITRRAPFGNASLIDEDVEINFQEETTPNDVVTVVATQPLTDNEEWFRM
HHHHHHCCCEEEEEECCCCCCCCEECCCCEEEECCCCCCCCEEEEEEECCCCCCCHHEEE
KPGEYALFHFGELIGNNHQALEDVAYAPKKVASQAPTEPLS
CCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]