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The map label for this gene is dapE

Identifier: 218710268

GI number: 218710268

Start: 2470937

End: 2472073

Strand: Direct

Name: dapE

Synonym: VS_2301

Alternate gene names: 218710268

Gene position: 2470937-2472073 (Clockwise)

Preceding gene: 218710267

Following gene: 218710269

Centisome position: 74.89

GC content: 47.76

Gene sequence:

>1137_bases
ATGACAGACAGCCCAACTTTGGCTCTGGCAAAAGACCTAATCAGCCGTCAATCGGTAACACCAGAAGATGCAGGCTGCCA
AGACCTGATGATTGAACGCTTAAAAGCGCTAGGTTTTGAAATCGAAGTGATGGTATTTGAAGATACGACGAACTTCTGGG
CTCGCCGTGGTACCGAAGCCCCCCTATTTGCTTTTGCTGGTCACACTGATGTTGTACCTGCAGGCCCAATTGAGCAGTGG
AACACTAAGCCATTCGAACCGACTATCATAGATGGTTTCCTGCACGGTCGCGGCGCGGCAGATATGAAAGGTTCTCTGGC
TTCAATGGTTGTGGCTGTAGAACAGTTCATTGCTAAGCACCCTGATCACACAGGCTCAATCGGTTTCCTTATCACGTCAG
ATGAAGAAGGCCCGTTCATCAACGGGACAGTGCGCGTTGTCGAAGCATTGATGGCTCGTGGTGAGAACATCGACATGTGT
ATTGTTGGCGAACCATCAAGTACTGAGTACGTAGGTGATGTCGTAAAGAACGGCCGTCGTGGTTCTATCACTGGCGATCT
AACGATTAAAGGCACACAAGGTCACGTTGCCTACCCTCACCTTGCGAACAACCCGGTACACAGCTCTCTGCTTGCGATCA
ATGAATTGGCGACGACCGAGTGGGACAAAGGTAATGATTATTTCCCACCAACCAGCTTCCAGATCCCAAATGTAAGTGCG
GGCACTGGCGCATCAAACGTGATTCCTGGTGAATTTAATGTTCAGTTTAACCTTCGCTTTAGTACAGAGTTAAACAACGA
CATCATCGTTGAAAGAATCACAAATACACTCGACAAGTACGATTTCGAATACGATTTAAAATGGACATTCAATGGCGACC
CGTTCTTAACCGACGCAGGCTCGTTGCTTGATGCTATTGTCGATGCAGTAGGTCACGTGAATGATGTTAAGCCGGCACTG
CTTACCACAGGTGGTACTTCTGACGGACGCTTTATTGCTCGCATGAAGGGACAAGTGGTAGAACTAGGCCCTGTGAATGC
AACCATTCACAAGGTTAACGAGTGCGTGAAAGTGGCTGATTTAGAGAAACTAACTGACATGTACGAAAGAACATTAGTGA
ACCTGTTCGCTAAGTAG

Upstream 100 bases:

>100_bases
GTAATCAGCTGTATTAGAACAGATAACAAAGCGTTGAACTTCCCCTGTTCAACGCCTGAAATTAATGACTAGACGTTTAA
TTTAAACAAGGAACTCAAGG

Downstream 100 bases:

>100_bases
TTTTTTCATCAGCGATTCATAGCATCTAATTATTGAGAGACGCGTTATGACACCAGAGCAGCTTACCGGACAATCAGATT
CTCATCTGGAACCTAGCCTG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTDSPTLALAKDLISRQSVTPEDAGCQDLMIERLKALGFEIEVMVFEDTTNFWARRGTEAPLFAFAGHTDVVPAGPIEQW
NTKPFEPTIIDGFLHGRGAADMKGSLASMVVAVEQFIAKHPDHTGSIGFLITSDEEGPFINGTVRVVEALMARGENIDMC
IVGEPSSTEYVGDVVKNGRRGSITGDLTIKGTQGHVAYPHLANNPVHSSLLAINELATTEWDKGNDYFPPTSFQIPNVSA
GTGASNVIPGEFNVQFNLRFSTELNNDIIVERITNTLDKYDFEYDLKWTFNGDPFLTDAGSLLDAIVDAVGHVNDVKPAL
LTTGGTSDGRFIARMKGQVVELGPVNATIHKVNECVKVADLEKLTDMYERTLVNLFAK

Sequences:

>Translated_378_residues
MTDSPTLALAKDLISRQSVTPEDAGCQDLMIERLKALGFEIEVMVFEDTTNFWARRGTEAPLFAFAGHTDVVPAGPIEQW
NTKPFEPTIIDGFLHGRGAADMKGSLASMVVAVEQFIAKHPDHTGSIGFLITSDEEGPFINGTVRVVEALMARGENIDMC
IVGEPSSTEYVGDVVKNGRRGSITGDLTIKGTQGHVAYPHLANNPVHSSLLAINELATTEWDKGNDYFPPTSFQIPNVSA
GTGASNVIPGEFNVQFNLRFSTELNNDIIVERITNTLDKYDFEYDLKWTFNGDPFLTDAGSLLDAIVDAVGHVNDVKPAL
LTTGGTSDGRFIARMKGQVVELGPVNATIHKVNECVKVADLEKLTDMYERTLVNLFAK
>Mature_377_residues
TDSPTLALAKDLISRQSVTPEDAGCQDLMIERLKALGFEIEVMVFEDTTNFWARRGTEAPLFAFAGHTDVVPAGPIEQWN
TKPFEPTIIDGFLHGRGAADMKGSLASMVVAVEQFIAKHPDHTGSIGFLITSDEEGPFINGTVRVVEALMARGENIDMCI
VGEPSSTEYVGDVVKNGRRGSITGDLTIKGTQGHVAYPHLANNPVHSSLLAINELATTEWDKGNDYFPPTSFQIPNVSAG
TGASNVIPGEFNVQFNLRFSTELNNDIIVERITNTLDKYDFEYDLKWTFNGDPFLTDAGSLLDAIVDAVGHVNDVKPALL
TTGGTSDGRFIARMKGQVVELGPVNATIHKVNECVKVADLEKLTDMYERTLVNLFAK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=374, Percent_Identity=63.1016042780749, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1790395, Length=243, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_VIBSL (B7VIL8)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417889.1
- ProteinModelPortal:   B7VIL8
- MEROPS:   M20.010
- GeneID:   7161843
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2301
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41136; Mature: 41005

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPTLALAKDLISRQSVTPEDAGCQDLMIERLKALGFEIEVMVFEDTTNFWARRGTEA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCCCC
PLFAFAGHTDVVPAGPIEQWNTKPFEPTIIDGFLHGRGAADMKGSLASMVVAVEQFIAKH
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
PDHTGSIGFLITSDEEGPFINGTVRVVEALMARGENIDMCIVGEPSSTEYVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCC
GSITGDLTIKGTQGHVAYPHLANNPVHSSLLAINELATTEWDKGNDYFPPTSFQIPNVSA
CCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCC
GTGASNVIPGEFNVQFNLRFSTELNNDIIVERITNTLDKYDFEYDLKWTFNGDPFLTDAG
CCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHH
SLLDAIVDAVGHVNDVKPALLTTGGTSDGRFIARMKGQVVELGPVNATIHKVNECVKVAD
HHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHH
LEKLTDMYERTLVNLFAK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSPTLALAKDLISRQSVTPEDAGCQDLMIERLKALGFEIEVMVFEDTTNFWARRGTEA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCCCC
PLFAFAGHTDVVPAGPIEQWNTKPFEPTIIDGFLHGRGAADMKGSLASMVVAVEQFIAKH
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
PDHTGSIGFLITSDEEGPFINGTVRVVEALMARGENIDMCIVGEPSSTEYVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCC
GSITGDLTIKGTQGHVAYPHLANNPVHSSLLAINELATTEWDKGNDYFPPTSFQIPNVSA
CCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCC
GTGASNVIPGEFNVQFNLRFSTELNNDIIVERITNTLDKYDFEYDLKWTFNGDPFLTDAG
CCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHH
SLLDAIVDAVGHVNDVKPALLTTGGTSDGRFIARMKGQVVELGPVNATIHKVNECVKVAD
HHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHH
LEKLTDMYERTLVNLFAK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA