Click here to switch to the map view.

The map label for this gene is crr [H]

Identifier: 218710259

GI number: 218710259

Start: 2463851

End: 2464360

Strand: Direct

Name: crr [H]

Synonym: VS_2292

Alternate gene names: 218710259

Gene position: 2463851-2464360 (Clockwise)

Preceding gene: 218710258

Following gene: 218710264

Centisome position: 74.68

GC content: 42.55

Gene sequence:

>510_bases
ATGGGTCTGTTTGACAAACTGAAAAAGCTTGTATCTGATGACAGCGCTGATGCTGGTGCAATCGAAATCATCGCACCTCT
TTCTGGTGAAATCGTAAACATCGAAGACGTGCCAGATGTAGTTTTCGCTGAAAAAATCGTTGGTGATGGCATTGCTATCA
AACCAGCTGGCGATAAAATGGTAGCTCCAGTTAACGGTACTATCGGTAAAATCTTCGAAACAAACCACGCATTCTCTATC
GAGTCTGACGACGGTATTGAGCTTTTCGTTCACTTCGGTATCGATACTGTTGAACTTAAAGGCGAAGGCTTCACTCGTGT
AGCTGAAGAAGGTCAATCTGTTAAAGCTGGTGACACAATCATCACTTTCGACCTTGCGCTTCTAGAAGAGAAAGCAAAAT
CTACGCTTACTCCAGTTGTTATCTCTAACATGGACGAAATCAAGGAGCTGAACAAGCTTTCTGGTTCTGTAACTGTTGGC
GAAACTCCAGTTCTTAAAGTAACTAAGTAA

Upstream 100 bases:

>100_bases
AGAGCTTAATGAAGTTAGACGGCTAAAAATAGTCGTCTAATTTGTTAGATTGGTATAGTATATTCTACAGAATAAAACTA
AACGTTAGGAGCATGACACA

Downstream 100 bases:

>100_bases
TCCCGATTACTTATCTGCTTTAATTAAAACGCTACCTAAGGGTAGCGTTTTTTGTGCCTGCTATATTTGTTCAATACCTC
AGATAGGCAGTACTGACTAT

Product: PTS system glucose-specific transporter subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MGLFDKLKKLVSDDSADAGAIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGDKMVAPVNGTIGKIFETNHAFSI
ESDDGIELFVHFGIDTVELKGEGFTRVAEEGQSVKAGDTIITFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVG
ETPVLKVTK

Sequences:

>Translated_169_residues
MGLFDKLKKLVSDDSADAGAIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGDKMVAPVNGTIGKIFETNHAFSI
ESDDGIELFVHFGIDTVELKGEGFTRVAEEGQSVKAGDTIITFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVG
ETPVLKVTK
>Mature_168_residues
GLFDKLKKLVSDDSADAGAIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGDKMVAPVNGTIGKIFETNHAFSIE
SDDGIELFVHFGIDTVELKGEGFTRVAEEGQSVKAGDTIITFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVGE
TPVLKVTK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=169, Percent_Identity=86.9822485207101, Blast_Score=289, Evalue=6e-80,
Organism=Escherichia coli, GI1786894, Length=149, Percent_Identity=46.9798657718121, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790159, Length=148, Percent_Identity=40.5405405405405, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17900; Mature: 17768

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFDKLKKLVSDDSADAGAIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGDKM
CCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEEECCCCEE
VAPVNGTIGKIFETNHAFSIESDDGIELFVHFGIDTVELKGEGFTRVAEEGQSVKAGDTI
EECCCCCEEEEEECCCEEEEECCCCEEEEEEECCEEEEECCCCCHHHHHCCCCEECCCEE
ITFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVGETPVLKVTK
EEEHHHHHHHHHHHCCCHHEECCHHHHHHHHHHCCCEEECCCCEEEECC
>Mature Secondary Structure 
GLFDKLKKLVSDDSADAGAIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGDKM
CHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEEECCCCEE
VAPVNGTIGKIFETNHAFSIESDDGIELFVHFGIDTVELKGEGFTRVAEEGQSVKAGDTI
EECCCCCEEEEEECCCEEEEECCCCEEEEEEECCEEEEECCCCCHHHHHCCCCEECCCEE
ITFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVGETPVLKVTK
EEEHHHHHHHHHHHCCCHHEECCHHHHHHHHHHCCCEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]