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The map label for this gene is ptsI [H]

Identifier: 218710258

GI number: 218710258

Start: 2462020

End: 2463744

Strand: Direct

Name: ptsI [H]

Synonym: VS_2291

Alternate gene names: 218710258

Gene position: 2462020-2463744 (Clockwise)

Preceding gene: 218710257

Following gene: 218710259

Centisome position: 74.62

GC content: 43.07

Gene sequence:

>1725_bases
ATGATTTCAGGCATCCTAGCATCTCCTGGTATTGCTTTCGGTAAAGCACTACTACTTCAAGAAGATGAAATTGTCCTAAA
CACTCAATCTATCTCAGACGATCAAGTTGAAGCAGAAGTTCAGCGTTTTTTTGACGCTCGTAACAAATCTTCTCAACAAC
TTGAAGTTGTAAAGCAAAAAGCACTTGAAACTTTTGGCGAAGAAAAAGAAGCAATCTTTGAAGGCCACATCATGCTGCTT
GAAGATGAAGAGCTAGAAGAAGAGATTTTAGCACTCATCAAGAAAGACAAAATGCACGCAGACAACGCGATCTACACAGT
GATCGAAGAGCAAGCTGTTGCACTTGAGTCGCTTGATGATGAGTACCTAAAAGAACGTGCGACTGATATCCGTGATATCG
GTACTCGCTTCGTTAAAAATGCACTAGGCATCAACATTGTATCTCTAGCAGATATCAATGAAGAAGTTATCCTAGTTGCT
TACGATTTAACGCCATCTGAAACGGCACAAATCAACCTAGACTACGTTCTTGGTTTTGCTTGTGACATCGGCGGTCGTAC
ATCTCATACTTCAATCATGGCACGTTCACTTGAGCTTCCAGCTATCGTTGGTACTAACGATATCACTAAGCAAGTTAAAA
ACGGCGACATGCTTGTGCTAGACGCGATGAACAACAAGATCATCATCAACCCTTCTGACGCTGAATTAGCAGAAGCTAAG
AAAATCAAATCTGATTTCGAAGCAGAAGCAGTTGAACTAGCAAAACTAAAAGACTTACATGCTGAAACTCTAGACGGTCA
CCGTGTAGAAGTTTGTGGCAACATCGGTACAGTAAAAGACTGTGACGGTATTCTGCGTAACGGCGGTGAAGGCGTTGGTC
TGTACCGTACTGAATTCCTATTCATGGACCGTACTGCACTTCCTACTGAAGAAGAGCAATACGTTGCTTACAAAGAAGTA
GCGGAAGCAATGGAAGGCGAGTCAGTGATTATCCGTACTATGGATATCGGTGGCGATAAAGACCTACCATACATGGACCT
TCCACAAGAAATGAACCCTTTCCTAGGCTGGCGTGCAGTACGTATCAGCTTGGATCGTCGTGAAATTCTACGTGACCAAC
TACGTGGTATCCTACGTGCATCTGCACACGGTAAACTACGCATCATGTTCCCAATGATCATTTCTGTTGAAGAGATCCGT
GAACTGAAAAAAGCAATCGAAGAGTACAAAGTTGAACTTCGCGCTGAAGGCCTAGCTTTCGATGAAGAAATCGAAATCGG
CGTAATGGTTGAGACTCCAGCAGCAGCTGCAATCGCACACCACCTAGCGAAAGAAGTATCTTTCTTCTCTATCGGTACTA
ACGACCTAACGCAATATACTCTTGCGGTAGACCGTGGTAACGAAATGATTTCTCACCTATACAACCCACTATCTCCAGCG
GTTCTTCTTGTTATCAAGCAAGTAATCGATGCTTCTCACAAAGAAGGTAAATGGACAGGTATGTGTGGCGAACTAGCAGG
TGATGAGCGTGCAACTCTACTTCTTCTTGGTATGGGTCTAGATGAGTTCTCTATGAGCGGTATCTCTATCCCTAAAGTTA
AGAAAGTAATCCGTAACTCTAACTTCGCTGAAGTTAAAGCTATGGCTGACGAAGCACTATCTCTACCTACAGCTGCAGAA
ATTGAAGCTTGTGTAGAAAAGTTCATCGCTGAGAAAACTCAGTAA

Upstream 100 bases:

>100_bases
ATAGATAGCAATTCGAAATAGATAGCAATAAGCGCGACATTAGTCGACGACTTAAAGTCACACATTCTCCCGTTTACAGT
TGAACAACTAAGGTAAGGCT

Downstream 100 bases:

>100_bases
TCACCAAGAGCTTAATGAAGTTAGACGGCTAAAAATAGTCGTCTAATTTGTTAGATTGGTATAGTATATTCTACAGAATA
AAACTAAACGTTAGGAGCAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MISGILASPGIAFGKALLLQEDEIVLNTQSISDDQVEAEVQRFFDARNKSSQQLEVVKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKDKMHADNAIYTVIEEQAVALESLDDEYLKERATDIRDIGTRFVKNALGINIVSLADINEEVILVA
YDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLVLDAMNNKIIINPSDAELAEAK
KIKSDFEAEAVELAKLKDLHAETLDGHRVEVCGNIGTVKDCDGILRNGGEGVGLYRTEFLFMDRTALPTEEEQYVAYKEV
AEAMEGESVIIRTMDIGGDKDLPYMDLPQEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDEEIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNSNFAEVKAMADEALSLPTAAE
IEACVEKFIAEKTQ

Sequences:

>Translated_574_residues
MISGILASPGIAFGKALLLQEDEIVLNTQSISDDQVEAEVQRFFDARNKSSQQLEVVKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKDKMHADNAIYTVIEEQAVALESLDDEYLKERATDIRDIGTRFVKNALGINIVSLADINEEVILVA
YDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLVLDAMNNKIIINPSDAELAEAK
KIKSDFEAEAVELAKLKDLHAETLDGHRVEVCGNIGTVKDCDGILRNGGEGVGLYRTEFLFMDRTALPTEEEQYVAYKEV
AEAMEGESVIIRTMDIGGDKDLPYMDLPQEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDEEIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNSNFAEVKAMADEALSLPTAAE
IEACVEKFIAEKTQ
>Mature_574_residues
MISGILASPGIAFGKALLLQEDEIVLNTQSISDDQVEAEVQRFFDARNKSSQQLEVVKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKDKMHADNAIYTVIEEQAVALESLDDEYLKERATDIRDIGTRFVKNALGINIVSLADINEEVILVA
YDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLVLDAMNNKIIINPSDAELAEAK
KIKSDFEAEAVELAKLKDLHAETLDGHRVEVCGNIGTVKDCDGILRNGGEGVGLYRTEFLFMDRTALPTEEEQYVAYKEV
AEAMEGESVIIRTMDIGGDKDLPYMDLPQEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDEEIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNSNFAEVKAMADEALSLPTAAE
IEACVEKFIAEKTQ

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=72.6003490401396, Blast_Score=830, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=38.474870017331, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI48994992, Length=503, Percent_Identity=38.3697813121272, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI1789193, Length=586, Percent_Identity=33.2764505119454, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1787994, Length=388, Percent_Identity=27.319587628866, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI226510935, Length=214, Percent_Identity=28.0373831775701, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63550; Mature: 63550

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALLLQEDEIVLNTQSISDDQVEAEVQRFFDARNKSSQQLEVVKQK
CCCCCCCCCCHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKKDKMHADNAIYTVIEEQAVALESLDD
HHHHHCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCH
EYLKERATDIRDIGTRFVKNALGINIVSLADINEEVILVAYDLTPSETAQINLDYVLGFA
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLVLDAMNNKIIINPSDAELAEAK
ECCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHH
KIKSDFEAEAVELAKLKDLHAETLDGHRVEVCGNIGTVKDCDGILRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEE
FMDRTALPTEEEQYVAYKEVAEAMEGESVIIRTMDIGGDKDLPYMDLPQEMNPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKKAIEEYKVELRAEGLAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEEIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCHHH
VLLVIKQVIDASHKEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNS
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NFAEVKAMADEALSLPTAAEIEACVEKFIAEKTQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGIAFGKALLLQEDEIVLNTQSISDDQVEAEVQRFFDARNKSSQQLEVVKQK
CCCCCCCCCCHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKKDKMHADNAIYTVIEEQAVALESLDD
HHHHHCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCH
EYLKERATDIRDIGTRFVKNALGINIVSLADINEEVILVAYDLTPSETAQINLDYVLGFA
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLVLDAMNNKIIINPSDAELAEAK
ECCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHH
KIKSDFEAEAVELAKLKDLHAETLDGHRVEVCGNIGTVKDCDGILRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEE
FMDRTALPTEEEQYVAYKEVAEAMEGESVIIRTMDIGGDKDLPYMDLPQEMNPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKKAIEEYKVELRAEGLAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEEIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCHHH
VLLVIKQVIDASHKEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNS
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NFAEVKAMADEALSLPTAAEIEACVEKFIAEKTQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]