Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 218710231

GI number: 218710231

Start: 2433849

End: 2435519

Strand: Direct

Name: asnB [H]

Synonym: VS_2264

Alternate gene names: 218710231

Gene position: 2433849-2435519 (Clockwise)

Preceding gene: 218710230

Following gene: 218710233

Centisome position: 73.77

GC content: 45.84

Gene sequence:

>1671_bases
ATGTGTTCAGTATTTGGCATTCTCGACATTAAAAGTGATGCCGCAGCACTTCGCCCTATTGCTTTAGAAATGTCTAAAAA
GCTTCGTCACCGTGGCCCAGACTGGTCTGGTATCTATGCCGGTGAAAAAGCTATCCTTGCTCACGAGCGTCTAGCTATCG
TCGGCTTAAACAGTGGTGCTCAACCACTATACAGCCAAGACAAAAAGCACATTCTTGCAGTTAATGGCGAAATCTATAAC
CACAAAGAACTTCGCGCTCGCTACGAAGATAAATACCAGTTCCAAACTGACTCTGATTGTGAAGTCATTCTTGCGCTTTA
CCAAGAGATGGGTGCCGACCTTTTAGAAGAACTTAACGGTATTTTCGCTTTCGTTTTATATGACGAAGAGAAAGACGAAT
ACCTAGTCGGCCGCGACCATATTGGCATCATCCCGCTTTACCAAGGCTACGATGAGCACGGCAACTATTACGTTGCTTCA
GAGATGAAAGCACTGGTTCCAGTTTGTAAGACGATCAGTGAGTTCCCTCCTGGTAGCTTCTACTCTTCTAAAGATGCAGA
ACCTCAACGCTACTACATCCGCGATTGGAACGAATACGCAGCCGTTCAAGGTAACAGCACAAGCAAAGAAGAGCTAACTG
AAGCGCTAGAAGCTGCTGTTAAGCGTCAACTAATGACTGACGTTCCTTACGGTGTACTTCTATCTGGTGGCCTTGATTCA
TCAATCACTTCTGCAGTCGCTAAGCGTTTTGCAGCAATGCGTATCGAAGATGATGAGCAATCAGAAGCTTGGTGGCCACA
ACTACACTCATTTGCTGTTGGCCTAGAAAATGCTCCCGACTTGATTGCTGCACGTGAAGTTGCGGACAAAATCGGTACTG
TACACCATGAGATGACTTACACCATTCAGGAAGGCTTAGATGCCATCCGTGATGTTATCTACCACATCGAAACTTACGAT
GTAACGACCATTCGTGCATCGACGCCAATGTACTTGCTTGCTCGTAAGATCAAAGCAATGGGTATCAAAATGGTACTTTC
TGGTGAAGGTGCTGATGAAATCTTTGGCGGCTACCTGTACTTCCATAAAGCGCCAAACGCAAAAGAGTTCCACGAAGAGA
CGGTACGTAAACTTCTTGCTCTAAGCATGTTTGACTGTGCCCGTGCAAACAAATCACTTGCAGCATGGGGAGTCGAAGGT
CGTGTACCATTCTTGGATAAAGAGTTCATCGATGTGGCTATGCGTTTAAACCCTGAAGACAAAATGTGCGGTAACGGTAA
AATGGAGAAACACATCCTACGTGAGTGTTTTGAAGACTACTTACCAGATTCAATCGCATGGCGCCAAAAAGAACAGTTCT
CTGATGGTGTTGGCTACGACTGGATTGATACATTGAAAGCAACGGCTGAAGAAAAAGTAACGGATCAACAAATGGAAGCT
GCTCAGTTCCGTTTCCCTTACAACACACCAACAACCAAAGAAGGTTATGCTTACCGTGAAATCTTTGAGGAGCTATTCCC
TCTAGAATCGGCGGCAGAGTGTGTACCTGGCGGCCCTTCAGTTGCCTGTTCATCAGCAAAAGCGATCGAGTGGGATGAGT
CATTCAAAAACTGTGTAGACCCATCAGGCCGTGCGGTACAAGCCGTTCACAACGACGCCTATAACGCTTAA

Upstream 100 bases:

>100_bases
ACCCCTTTATATTATTTTTTTGCTCATATTCGGTTGCTTTTTTTAGCCAGAATGGCAAATTGAATACAGTTGATTTAGAA
ATTATTTAAAAGGAAGTTCT

Downstream 100 bases:

>100_bases
GCGTTTGAAAAATCAAATACTAAAAAAGGCGCATTATGCGCCTTTTTGTTTTAAGCCTAGTTTTTACTTTTTTAATATTT
GACTTATACGTGAGCTTGTA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MCSVFGILDIKSDAAALRPIALEMSKKLRHRGPDWSGIYAGEKAILAHERLAIVGLNSGAQPLYSQDKKHILAVNGEIYN
HKELRARYEDKYQFQTDSDCEVILALYQEMGADLLEELNGIFAFVLYDEEKDEYLVGRDHIGIIPLYQGYDEHGNYYVAS
EMKALVPVCKTISEFPPGSFYSSKDAEPQRYYIRDWNEYAAVQGNSTSKEELTEALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEQSEAWWPQLHSFAVGLENAPDLIAAREVADKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLARKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALSMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPEDKMCGNGKMEKHILRECFEDYLPDSIAWRQKEQFSDGVGYDWIDTLKATAEEKVTDQQMEA
AQFRFPYNTPTTKEGYAYREIFEELFPLESAAECVPGGPSVACSSAKAIEWDESFKNCVDPSGRAVQAVHNDAYNA

Sequences:

>Translated_556_residues
MCSVFGILDIKSDAAALRPIALEMSKKLRHRGPDWSGIYAGEKAILAHERLAIVGLNSGAQPLYSQDKKHILAVNGEIYN
HKELRARYEDKYQFQTDSDCEVILALYQEMGADLLEELNGIFAFVLYDEEKDEYLVGRDHIGIIPLYQGYDEHGNYYVAS
EMKALVPVCKTISEFPPGSFYSSKDAEPQRYYIRDWNEYAAVQGNSTSKEELTEALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEQSEAWWPQLHSFAVGLENAPDLIAAREVADKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLARKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALSMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPEDKMCGNGKMEKHILRECFEDYLPDSIAWRQKEQFSDGVGYDWIDTLKATAEEKVTDQQMEA
AQFRFPYNTPTTKEGYAYREIFEELFPLESAAECVPGGPSVACSSAKAIEWDESFKNCVDPSGRAVQAVHNDAYNA
>Mature_556_residues
MCSVFGILDIKSDAAALRPIALEMSKKLRHRGPDWSGIYAGEKAILAHERLAIVGLNSGAQPLYSQDKKHILAVNGEIYN
HKELRARYEDKYQFQTDSDCEVILALYQEMGADLLEELNGIFAFVLYDEEKDEYLVGRDHIGIIPLYQGYDEHGNYYVAS
EMKALVPVCKTISEFPPGSFYSSKDAEPQRYYIRDWNEYAAVQGNSTSKEELTEALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEQSEAWWPQLHSFAVGLENAPDLIAAREVADKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLARKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALSMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPEDKMCGNGKMEKHILRECFEDYLPDSIAWRQKEQFSDGVGYDWIDTLKATAEEKVTDQQMEA
AQFRFPYNTPTTKEGYAYREIFEELFPLESAAECVPGGPSVACSSAKAIEWDESFKNCVDPSGRAVQAVHNDAYNA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=544, Percent_Identity=38.4191176470588, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI168229252, Length=544, Percent_Identity=38.4191176470588, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI168229250, Length=544, Percent_Identity=38.4191176470588, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI296010848, Length=557, Percent_Identity=38.4201077199282, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI296010852, Length=489, Percent_Identity=39.0593047034765, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI296010850, Length=489, Percent_Identity=39.0593047034765, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1786889, Length=555, Percent_Identity=75.8558558558559, Blast_Score=908, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=571, Percent_Identity=43.4325744308231, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=43.4704830053667, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17560178, Length=524, Percent_Identity=35.4961832061069, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=36.6568914956012, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=51.0091743119266, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=50, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=47.6603119584055, Blast_Score=489, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62565; Mature: 62565

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSVFGILDIKSDAAALRPIALEMSKKLRHRGPDWSGIYAGEKAILAHERLAIVGLNSGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHEEEECCEEEEECCCCC
QPLYSQDKKHILAVNGEIYNHKELRARYEDKYQFQTDSDCEVILALYQEMGADLLEELNG
CCHHCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
IFAFVLYDEEKDEYLVGRDHIGIIPLYQGYDEHGNYYVASEMKALVPVCKTISEFPPGSF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDAEPQRYYIRDWNEYAAVQGNSTSKEELTEALEAAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCCCEEHEECCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKRFAAMRIEDDEQSEAWWPQLHSFAVGLENAPDLIAAREVADKIGTVHHEMTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMYLLARKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAKEFHEETVRKLLALSMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPED
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHEECCCC
KMCGNGKMEKHILRECFEDYLPDSIAWRQKEQFSDGVGYDWIDTLKATAEEKVTDQQMEA
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQFRFPYNTPTTKEGYAYREIFEELFPLESAAECVPGGPSVACSSAKAIEWDESFKNCVD
HHEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVQAVHNDAYNA
CCCCEEHHHHHCCCCC
>Mature Secondary Structure
MCSVFGILDIKSDAAALRPIALEMSKKLRHRGPDWSGIYAGEKAILAHERLAIVGLNSGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHEEEECCEEEEECCCCC
QPLYSQDKKHILAVNGEIYNHKELRARYEDKYQFQTDSDCEVILALYQEMGADLLEELNG
CCHHCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
IFAFVLYDEEKDEYLVGRDHIGIIPLYQGYDEHGNYYVASEMKALVPVCKTISEFPPGSF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDAEPQRYYIRDWNEYAAVQGNSTSKEELTEALEAAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCCCEEHEECCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKRFAAMRIEDDEQSEAWWPQLHSFAVGLENAPDLIAAREVADKIGTVHHEMTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMYLLARKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAKEFHEETVRKLLALSMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPED
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHEECCCC
KMCGNGKMEKHILRECFEDYLPDSIAWRQKEQFSDGVGYDWIDTLKATAEEKVTDQQMEA
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQFRFPYNTPTTKEGYAYREIFEELFPLESAAECVPGGPSVACSSAKAIEWDESFKNCVD
HHEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVQAVHNDAYNA
CCCCEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]