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The map label for this gene is hupB [H]

Identifier: 218710171

GI number: 218710171

Start: 2350549

End: 2350842

Strand: Reverse

Name: hupB [H]

Synonym: VS_2201

Alternate gene names: 218710171

Gene position: 2350842-2350549 (Counterclockwise)

Preceding gene: 218710172

Following gene: 218710170

Centisome position: 71.25

GC content: 45.24

Gene sequence:

>294_bases
ATGACACATAGGGGAAAAACAGTGAATAAAACACAACTAGTAGAATCAATCGCAGAAAACGCAGACATCTCTAAAGCTTC
AGCTGGTCGCGCTCTAGACGCTTTCATCGAAGCAGTTGGCACAACGCTACAATCAGGCGACCAAGTTGCACTTGTTGGCT
TTGGTACTTTCAGTGTTCGTACTCGTGCTGCTCGTACAGGTCGTAACCCAAAAACTGGTGAAGAGATCCAAATCGCAGAA
GCTAAAGTACCTGGCTTCAAAGCGGGTAAAGCACTTAAAGACGCATGTAACTAA

Upstream 100 bases:

>100_bases
ACGCTAAGTTATTCGACTAAAGCGGCAGCCCTTTTGTAATAAGGCTTGCGGCTAAATTAAAACCAAAATGGAACGTACAG
TCATCGAGAAGTAGTCACAG

Downstream 100 bases:

>100_bases
TTTTTGTTGCTGAAACCTCAAGTTTTTGTGAGGAGAAGGCAGTAATATGCGTTTACTGTTTGAACAATGATGTTTCATTT
TCAAACAGTATATGTGCAAA

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MTHRGKTVNKTQLVESIAENADISKASAGRALDAFIEAVGTTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIQIAE
AKVPGFKAGKALKDACN

Sequences:

>Translated_97_residues
MTHRGKTVNKTQLVESIAENADISKASAGRALDAFIEAVGTTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIQIAE
AKVPGFKAGKALKDACN
>Mature_96_residues
THRGKTVNKTQLVESIAENADISKASAGRALDAFIEAVGTTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIQIAEA
KVPGFKAGKALKDACN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=70, Blast_Score=128, Evalue=5e-32,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=58.4269662921348, Blast_Score=105, Evalue=6e-25,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=38.4615384615385, Blast_Score=78, Evalue=9e-17,
Organism=Escherichia coli, GI1788005, Length=86, Percent_Identity=38.3720930232558, Blast_Score=70, Evalue=2e-14,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10147; Mature: 10015

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRGKTVNKTQLVESIAENADISKASAGRALDAFIEAVGTTLQSGDQVALVGFGTFSVR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TRAARTGRNPKTGEEIQIAEAKVPGFKAGKALKDACN
HHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
THRGKTVNKTQLVESIAENADISKASAGRALDAFIEAVGTTLQSGDQVALVGFGTFSVR
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TRAARTGRNPKTGEEIQIAEAKVPGFKAGKALKDACN
HHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]