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The map label for this gene is ybfI [H]

Identifier: 218710137

GI number: 218710137

Start: 2312502

End: 2313347

Strand: Reverse

Name: ybfI [H]

Synonym: VS_2159

Alternate gene names: 218710137

Gene position: 2313347-2312502 (Counterclockwise)

Preceding gene: 218710140

Following gene: 218710136

Centisome position: 70.12

GC content: 46.57

Gene sequence:

>846_bases
TTGGGGATTGTTATGGAGAACAAGAAACGCTCTAAAGAAAAGGCCGAATATAAGATCACAGAAGAGCTCGGTGGCCTTGA
AACCCTTAATGCTGAATACGAAAAGCAGAACTTCTCACGCCACAGCCACGAGGGCTACACGCTTGGAGTCATCGAACAAG
GCGCTCAGCGTTTCTATCGAACGGGCGGGCATCATATTGCACCACAAAATGCGATTATTTTGGTCAATGCCGACGAAGTT
CATAGCGGTCACTCTGCCACTGAAGGGGGTTGGGCGTATCGAGCCATGTATCCTCTCCCAGAGCAACTTGCCAAGATCAC
TCAAGAGCTTAACCTACCGAATTACGGTGCGCCTTACTTCCCAAGAGCGGTGGTTGAAGACCCTGAACTCGCCAATCAAT
TGAGATTGGTATTTAACACGATTGATGAATCCGACAATCGTTTGTTAAGAGAAACCTTGATGTACGGGATGTTGGTTAAG
CTGATTAGCCGACACGGAAAATCGCCGCTTAACCCTCAACTCGACGGGAAAACTCAGCGCCAGCTTGTGTTGGTGAAAGA
GTTTTTAGATGATTTTCCACAAGCCGATGTGTCCTTAGAGGAGCTGTCTAAGCTAGCGGCTTTGAGCCCGTTTCACTTAG
TGCGTTCTTTTCAAAAAGAGTTCGGCCTTCCACCACATGCGTATCAGATTCAGTCTAGATTGAGGTTATCGCGTAAGTTA
TTGAAGCAAGGACATACGATTTCAGATACGGCTCAAGAATGCGGTTTTCATGATCAAAGCCATTTTCATCGACATTTCAA
AAAGGCCAATGGTTACACGCCGGGGCAATACATCAAAATGCTCTAA

Upstream 100 bases:

>100_bases
CCAACGATAAAAACAGTATTAACAAACAAAAGCGCAATTTTGTACAAAAGCTGAAAGAGCCTATAGAGTAGACTGATAAG
TCGCTCGATAATTAACAGGC

Downstream 100 bases:

>100_bases
ACTTGCGTGGCTAAGGCATGGCCAACTCGTGTTAAGTTCGGTTAAGTGTGGCAAACAGAGGGACATGAAATCGAGCAAAT
TTGTACAATCGAACCGACTC

Product: AraC-type DNA-binding domain-containing protein

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MGIVMENKKRSKEKAEYKITEELGGLETLNAEYEKQNFSRHSHEGYTLGVIEQGAQRFYRTGGHHIAPQNAIILVNADEV
HSGHSATEGGWAYRAMYPLPEQLAKITQELNLPNYGAPYFPRAVVEDPELANQLRLVFNTIDESDNRLLRETLMYGMLVK
LISRHGKSPLNPQLDGKTQRQLVLVKEFLDDFPQADVSLEELSKLAALSPFHLVRSFQKEFGLPPHAYQIQSRLRLSRKL
LKQGHTISDTAQECGFHDQSHFHRHFKKANGYTPGQYIKML

Sequences:

>Translated_281_residues
MGIVMENKKRSKEKAEYKITEELGGLETLNAEYEKQNFSRHSHEGYTLGVIEQGAQRFYRTGGHHIAPQNAIILVNADEV
HSGHSATEGGWAYRAMYPLPEQLAKITQELNLPNYGAPYFPRAVVEDPELANQLRLVFNTIDESDNRLLRETLMYGMLVK
LISRHGKSPLNPQLDGKTQRQLVLVKEFLDDFPQADVSLEELSKLAALSPFHLVRSFQKEFGLPPHAYQIQSRLRLSRKL
LKQGHTISDTAQECGFHDQSHFHRHFKKANGYTPGQYIKML
>Mature_280_residues
GIVMENKKRSKEKAEYKITEELGGLETLNAEYEKQNFSRHSHEGYTLGVIEQGAQRFYRTGGHHIAPQNAIILVNADEVH
SGHSATEGGWAYRAMYPLPEQLAKITQELNLPNYGAPYFPRAVVEDPELANQLRLVFNTIDESDNRLLRETLMYGMLVKL
ISRHGKSPLNPQLDGKTQRQLVLVKEFLDDFPQADVSLEELSKLAALSPFHLVRSFQKEFGLPPHAYQIQSRLRLSRKLL
KQGHTISDTAQECGFHDQSHFHRHFKKANGYTPGQYIKML

Specific function: Repair Of Alkylated Guanine In DNA By Stoichiometrically Transferring The Alkyl Group At The O-6 Position To A Cysteine Residue In The Enzyme. This Is A Suicide Reaction: The Enzyme Is Irreversibly Inactivated. Can Also Repair O-4-Methylthymine. The Meth

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 32081; Mature: 31950

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIVMENKKRSKEKAEYKITEELGGLETLNAEYEKQNFSRHSHEGYTLGVIEQGAQRFYR
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TGGHHIAPQNAIILVNADEVHSGHSATEGGWAYRAMYPLPEQLAKITQELNLPNYGAPYF
CCCCEECCCCEEEEEECHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PRAVVEDPELANQLRLVFNTIDESDNRLLRETLMYGMLVKLISRHGKSPLNPQLDGKTQR
CCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QLVLVKEFLDDFPQADVSLEELSKLAALSPFHLVRSFQKEFGLPPHAYQIQSRLRLSRKL
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKQGHTISDTAQECGFHDQSHFHRHFKKANGYTPGQYIKML
HHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure 
GIVMENKKRSKEKAEYKITEELGGLETLNAEYEKQNFSRHSHEGYTLGVIEQGAQRFYR
CCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TGGHHIAPQNAIILVNADEVHSGHSATEGGWAYRAMYPLPEQLAKITQELNLPNYGAPYF
CCCCEECCCCEEEEEECHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PRAVVEDPELANQLRLVFNTIDESDNRLLRETLMYGMLVKLISRHGKSPLNPQLDGKTQR
CCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QLVLVKEFLDDFPQADVSLEELSKLAALSPFHLVRSFQKEFGLPPHAYQIQSRLRLSRKL
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKQGHTISDTAQECGFHDQSHFHRHFKKANGYTPGQYIKML
HHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]