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The map label for this gene is cysB [H]

Identifier: 218710030

GI number: 218710030

Start: 2200651

End: 2201625

Strand: Reverse

Name: cysB [H]

Synonym: VS_2050

Alternate gene names: 218710030

Gene position: 2201625-2200651 (Counterclockwise)

Preceding gene: 218710033

Following gene: 218710029

Centisome position: 66.73

GC content: 44.1

Gene sequence:

>975_bases
ATGAAGTTACAGCAACTGAAGTATATTGTTGAGGTTCTAAACCATAACCTAAATGTTTCAGCGACAGCAGAGAGTTTGTA
CACATCTCAGCCCGGCATAAGTAAACAGGTTAGATTACTTGAAGATGAGCTAGGCATTCAAATATTTGAGCGTAGCGGTA
AGCATTTAACTCAGGTTACACAAGCCGGTGAGGATATTATTCGTATCTCTCAAGAGATTTTGGCTCGTGTTGAGAGTATT
AAAGCGGTTGCGGGTGAGCACACTCATCCTGAGATGGGCACATTGAATATTTCAACCACTCATACTCAAGCTCGTTATGC
ACTCCCTGATGTGATTAAAGGTTTCACGGCGCGTTATCCAAAAGTGTCGCTTCACATGCATCAAGGCACGCCAAGCCAGA
TGTCAGAAGCGGTAGCTAAGGGTACAGCGAACTTTGCGATTGCAACGGAAGCGCTACACCTTTATCAAGATGCAATTATG
CTGCCTTGTTATCATTGGAACCGTTCGATCGTGGTAACGAAAGATCACCCTCTTGCACAGAAACGAAATATCTCGATTGA
AGATCTTGCCGCTTATTCTCTCGTGACTTATGTATTTGGTTTTACTGGCCGTTCCGAGCTGGATACAGCATTTAACAAAG
TAGGCTTAACACCGCGTGTTGTGTTTACCGCGACGGATGCTGATGTGATTAAAACTTATGTTCGGATGGGAATCGGGGTG
GGTGTAATTGCAAGCATGGCGATTGACCACGAGCAAGATACAGACTTGGTTGCTATTGACGCGAGCCACTTGTTTGGTGC
GAGTACCACAAGTATTGGCTTTAGAAAGGGTACTTTCTTACGTTCTTACATGTTCGATTTCATGGAGCGTTTTGCACCGC
ACTTAACTCGCCCAGTAGTTGAGCAAGCTATTTCTTTGAAATCGAATGAAGAGATCGAAGAGATGTTTAAAGACATCGAC
TTACCCGTTCGCTAA

Upstream 100 bases:

>100_bases
TCTCGAAGCGTTATAACTATTAATTATATTACACGAGACTATAACGCTCTATTTGCAAATAAGTTAGCGCAGAAATGAGT
AAAGGATTACGAGGTTAGAT

Downstream 100 bases:

>100_bases
GGGTCATAGTCTTACCTAGATTCCCAATTCGGCCTGTTTACAGGCCGTTTTCATTTAAGCTCATTTGTGGTTTTATTGCT
GGTAGGTTGTCTTTATACAA

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLKYIVEVLNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTQAGEDIIRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFTARYPKVSLHMHQGTPSQMSEAVAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQKRNISIEDLAAYSLVTYVFGFTGRSELDTAFNKVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAIDHEQDTDLVAIDASHLFGASTTSIGFRKGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNEEIEEMFKDID
LPVR

Sequences:

>Translated_324_residues
MKLQQLKYIVEVLNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTQAGEDIIRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFTARYPKVSLHMHQGTPSQMSEAVAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQKRNISIEDLAAYSLVTYVFGFTGRSELDTAFNKVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAIDHEQDTDLVAIDASHLFGASTTSIGFRKGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNEEIEEMFKDID
LPVR
>Mature_324_residues
MKLQQLKYIVEVLNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTQAGEDIIRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFTARYPKVSLHMHQGTPSQMSEAVAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQKRNISIEDLAAYSLVTYVFGFTGRSELDTAFNKVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAIDHEQDTDLVAIDASHLFGASTTSIGFRKGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNEEIEEMFKDID
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=75.4658385093168, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=38.9776357827476, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=25.8741258741259, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI157672245, Length=252, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=27.6422764227642, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=234, Percent_Identity=23.9316239316239, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=240, Percent_Identity=24.1666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=271, Percent_Identity=20.6642066420664, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=23.7931034482759, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=262, Percent_Identity=23.6641221374046, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=23.9043824701195, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36082; Mature: 36082

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLKYIVEVLNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHH
QAGEDIIRISQEILARVESIKAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFTARYP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
KVSLHMHQGTPSQMSEAVAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVTKDHPLAQ
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCHH
KRNISIEDLAAYSLVTYVFGFTGRSELDTAFNKVGLTPRVVFTATDADVIKTYVRMGIGV
HCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAIDHEQDTDLVAIDASHLFGASTTSIGFRKGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNEEIEEMFKDIDLPVR
HHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLKYIVEVLNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHH
QAGEDIIRISQEILARVESIKAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFTARYP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
KVSLHMHQGTPSQMSEAVAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVTKDHPLAQ
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCHH
KRNISIEDLAAYSLVTYVFGFTGRSELDTAFNKVGLTPRVVFTATDADVIKTYVRMGIGV
HCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAIDHEQDTDLVAIDASHLFGASTTSIGFRKGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNEEIEEMFKDIDLPVR
HHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]