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The map label for this gene is purB [H]

Identifier: 218710014

GI number: 218710014

Start: 2179953

End: 2181323

Strand: Direct

Name: purB [H]

Synonym: VS_2034

Alternate gene names: 218710014

Gene position: 2179953-2181323 (Clockwise)

Preceding gene: 218710013

Following gene: 218710016

Centisome position: 66.07

GC content: 45.81

Gene sequence:

>1371_bases
ATGGAACTGTCAGCATTGACTGCTGTTTCACCAGTAGACGGCCGTTACGGAAGCAAGACTGTTGCATTACGCAGCATCTT
TAGTGAGTTTGGCCTACTAAAGTACCGCTCTATCGTTGAAGTTCGTTGGTTACAAAAGCTTGCAGCTACAGATGCAATCA
AAGAAGTACCAGCGTTCAGTGCAGAAGCTAACCAGTTTCTTGATGAACTAGCTGCTAACTTCAGTGAAGAAGATGCTCTA
CGTATCAAAGAGATCGAGCGCACGACTAACCACGACGTTAAAGCGGTTGAGTACTTCTTGAAAGAGAAAGTTGCTGGCGT
TCCTGAACTTCACGCTGTAAACGAATTTATTCACTTTGCATGTACTTCTGAAGACATCAACAACACATCTCACGCGCTTA
TGCTTAAAGAAGCTCGTGACACAGTGATTCTTCCAGAAATCCGTAACGTAATTGATGCTATCAAAGCGCTTGCGAACGAG
TACCGTGACATTCCATTACTGTCTCGTACACACGGTCAGCCTGCTTCTCCTTCTACTATGGGTAAAGAGATGGCTAACGT
TGCGTACCGTATGGAACGTCAATACAAGCAAATCGAAAACGTTGAGATCCTAGCGAAAATCAACGGTGCTGTAGGTAACT
ACAACGCACACCTTTCTGCATACCCAGAAGTTGAATGGCACCAGTTCAGCGAAGAGTTCATCACTGAATCTCTTGGCGTA
ACTTGGAACCCGTACACAACTCAAATCGAACCTCACGATTACATCGCTGAGCTTTTTGATGCAGTTGCACGTTTCAACAC
TATCCTTCTAGACTTCGACCGTGACGTTTGGGGCTACATCGCTCTTGGTCACTTCAAGCAGAAGACTATTGCTGGCGAAA
TCGGTTCTTCTACAATGCCGCATAAAGTTAACCCAATTGACTTCGAAAACTCTGAAGGCAACCTTGGTCTAGCAAACGCA
GTATTCAGCCACCTTGCACAAAAGCTTCCAGTTTCTCGCTGGCAGCGTGACCTTACTGACTCTACGGTTCTTCGTAACCT
AGGTGTTGGTGTTGGCTACGCTATCATTGCATACACTTCAACTCTGAAAGGTATTAGCAAGCTAGAAGTTAACCGTGAAG
CGCTACTTGCTGAACTAGACAAGAACTGGGAAGTACTAGCAGAACCAGTACAAACAGTAATGCGCCGTTACGGCATCGAG
AAGCCATACGAGAAGCTAAAAGAGCTAACTCGTGGTAAACGTGTAGATGGCGAAGGCATGCGTGCATTCATCGACGGCCT
TGAAATCCCTGCAGACGAGAAAGTTCGTCTGAAAGAGATGACTCCAGCGAACTACATCGGTCAAGCAATCGAGCTAACTG
ACAAGCTGTAA

Upstream 100 bases:

>100_bases
CACAACTCTCACGCAAACGATTGCGCAATATGCGTGACAATTGAGATTGTTACTGGTATAAACCTCTCAAAATTTAGAAA
ATTCAATTCAGGAGAACACC

Downstream 100 bases:

>100_bases
GATTCGAATTTCGAATCACAGAATAGACTAAGGCTTCCCATGTGGAAGCCTTTTTGTTGAGCGGAAGAAAGTGTGCTTAG
TACGCGCTGTCATCAAAAGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSALTAVSPVDGRYGSKTVALRSIFSEFGLLKYRSIVEVRWLQKLAATDAIKEVPAFSAEANQFLDELAANFSEEDAL
RIKEIERTTNHDVKAVEYFLKEKVAGVPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPEVEWHQFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAVARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFSHLAQKLPVSRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNREALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKVRLKEMTPANYIGQAIELTDKL

Sequences:

>Translated_456_residues
MELSALTAVSPVDGRYGSKTVALRSIFSEFGLLKYRSIVEVRWLQKLAATDAIKEVPAFSAEANQFLDELAANFSEEDAL
RIKEIERTTNHDVKAVEYFLKEKVAGVPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPEVEWHQFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAVARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFSHLAQKLPVSRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNREALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKVRLKEMTPANYIGQAIELTDKL
>Mature_456_residues
MELSALTAVSPVDGRYGSKTVALRSIFSEFGLLKYRSIVEVRWLQKLAATDAIKEVPAFSAEANQFLDELAANFSEEDAL
RIKEIERTTNHDVKAVEYFLKEKVAGVPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPEVEWHQFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAVARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFSHLAQKLPVSRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNREALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKVRLKEMTPANYIGQAIELTDKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=82.4561403508772, Blast_Score=789, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323391, Length=354, Percent_Identity=26.271186440678, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647570, Length=271, Percent_Identity=25.830258302583, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51307; Mature: 51307

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTAVSPVDGRYGSKTVALRSIFSEFGLLKYRSIVEVRWLQKLAATDAIKEVPAFS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEANQFLDELAANFSEEDALRIKEIERTTNHDVKAVEYFLKEKVAGVPELHAVNEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPEVEWHQFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
TWNPYTTQIEPHDYIAELFDAVARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAVFSHLAQKLPVSRWQRDLTDSTVLRNLGVGVGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLKGISKLEVNREALLAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDGEGM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
RAFIDGLEIPADEKVRLKEMTPANYIGQAIELTDKL
HHHHHCCCCCCCCCEEHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MELSALTAVSPVDGRYGSKTVALRSIFSEFGLLKYRSIVEVRWLQKLAATDAIKEVPAFS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEANQFLDELAANFSEEDALRIKEIERTTNHDVKAVEYFLKEKVAGVPELHAVNEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPEVEWHQFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
TWNPYTTQIEPHDYIAELFDAVARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAVFSHLAQKLPVSRWQRDLTDSTVLRNLGVGVGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLKGISKLEVNREALLAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDGEGM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
RAFIDGLEIPADEKVRLKEMTPANYIGQAIELTDKL
HHHHHCCCCCCCCCEEHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]