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The map label for this gene is trpG [H]

Identifier: 218709948

GI number: 218709948

Start: 2110711

End: 2111355

Strand: Direct

Name: trpG [H]

Synonym: VS_1967

Alternate gene names: 218709948

Gene position: 2110711-2111355 (Clockwise)

Preceding gene: 218709947

Following gene: 218709949

Centisome position: 63.97

GC content: 46.82

Gene sequence:

>645_bases
ATGGCTGATATTGTATTCATCGATAACTTCGACTCGTTCACTTACAACCTTGTAGACCAGTTCCGTTCATTAGGTCACAG
CGTGAAAATTTACCGTAACAATATTCCTGCAAAGGTAGTTGAAGCGGCGATCAACGAACTAGACAACCCTGTTGCACTGC
TTTCACCAGGCCCTGGCGCTCCAGCAGATGCGGGCTGTATGCCAGAGCTGATTCAGCTTCTAAAAGGTAAAGTACCAATG
ATTGGTATTTGCTTAGGCCACCAAGCGATTGTTGAGGCTTACGGCGGCACCGTTGCAGGCGCTGGCGAAATCATTCATGG
TAAGGTGTCGATGATGGAACACCAAAACCATGCGACTTACCAAGGCTTACCTTCACCACTGGCTATTGCTCGTTACCACT
CTTTAGTGGCAACGCATGTATCTGATAGCCTAACGGTGACAGCTGAAGTCGATGATTTGGTGATGTCTGTGGTTCAAGAA
CAAGACAAGGTTTGTGGATTCCAATTCCACCCTGAATCAATTATGACGACCTACGGTGCAACGCTTCTAGCAAACGCTAT
CGAATGGGCTCTTGAGAAACCTCTTCATTTAGAGAAAAGCTCTCCCCTTGAGAAGACAGCGACTCTTGACCAAAAGACTA
ACTAG

Upstream 100 bases:

>100_bases
AAGCAGAAGCAGATGAAACTCGCGGCAAAGCTCAAGCGGTCATCTCTGCGATTCAAGCAGCTCATACTAAAAACCTAACA
ACTAAGAAGGAGTCGTAATC

Downstream 100 bases:

>100_bases
GAAACGATCATGGAACAGATTAATAAACTTTACGAACAGCAGTCTCTTACTCAAGAAGAAAGCCAACAATTGTTTGATGT
GATCATCAAGGGTGAGCTAG

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MADIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNNIPAKVVEAAINELDNPVALLSPGPGAPADAGCMPELIQLLKGKVPM
IGICLGHQAIVEAYGGTVAGAGEIIHGKVSMMEHQNHATYQGLPSPLAIARYHSLVATHVSDSLTVTAEVDDLVMSVVQE
QDKVCGFQFHPESIMTTYGATLLANAIEWALEKPLHLEKSSPLEKTATLDQKTN

Sequences:

>Translated_214_residues
MADIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNNIPAKVVEAAINELDNPVALLSPGPGAPADAGCMPELIQLLKGKVPM
IGICLGHQAIVEAYGGTVAGAGEIIHGKVSMMEHQNHATYQGLPSPLAIARYHSLVATHVSDSLTVTAEVDDLVMSVVQE
QDKVCGFQFHPESIMTTYGATLLANAIEWALEKPLHLEKSSPLEKTATLDQKTN
>Mature_213_residues
ADIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNNIPAKVVEAAINELDNPVALLSPGPGAPADAGCMPELIQLLKGKVPMI
GICLGHQAIVEAYGGTVAGAGEIIHGKVSMMEHQNHATYQGLPSPLAIARYHSLVATHVSDSLTVTAEVDDLVMSVVQEQ
DKVCGFQFHPESIMTTYGATLLANAIEWALEKPLHLEKSSPLEKTATLDQKTN

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=204, Percent_Identity=57.3529411764706, Blast_Score=253, Evalue=5e-69,
Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=40.1069518716578, Blast_Score=140, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322638, Length=188, Percent_Identity=39.8936170212766, Blast_Score=144, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6324361, Length=214, Percent_Identity=30.8411214953271, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 23079; Mature: 22947

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNNIPAKVVEAAINELDNPVALLSPGPGA
CCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
PADAGCMPELIQLLKGKVPMIGICLGHQAIVEAYGGTVAGAGEIIHGKVSMMEHQNHATY
CCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCC
QGLPSPLAIARYHSLVATHVSDSLTVTAEVDDLVMSVVQEQDKVCGFQFHPESIMTTYGA
CCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHH
TLLANAIEWALEKPLHLEKSSPLEKTATLDQKTN
HHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
ADIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNNIPAKVVEAAINELDNPVALLSPGPGA
CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
PADAGCMPELIQLLKGKVPMIGICLGHQAIVEAYGGTVAGAGEIIHGKVSMMEHQNHATY
CCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCC
QGLPSPLAIARYHSLVATHVSDSLTVTAEVDDLVMSVVQEQDKVCGFQFHPESIMTTYGA
CCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHH
TLLANAIEWALEKPLHLEKSSPLEKTATLDQKTN
HHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]