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The map label for this gene is trpE [H]

Identifier: 218709947

GI number: 218709947

Start: 2109071

End: 2110708

Strand: Direct

Name: trpE [H]

Synonym: VS_1966

Alternate gene names: 218709947

Gene position: 2109071-2110708 (Clockwise)

Preceding gene: 218709943

Following gene: 218709948

Centisome position: 63.92

GC content: 45.97

Gene sequence:

>1638_bases
ATGCGACTATGTACGGCAGGAAATTTAAAGAATCATAAAGGAGGTCTTGTGAACAAGGCCATTGAAATCAAAAAGCTGGG
AACCATTGAGGTCATCAATTCTTCCGTTCCTTACTCGCAAGATCCAACCAGTGTTTTTCATACTCTGTGTGAAAACAAAA
CAGACAGTCTGTTGTTGGAATCTGCTGAGATTGAATCTAAACAGAACTTGACAAGTCTACTCCTTATCGACTCTGCTGTT
CGCATCGTATGTCGCGGACATGAAGTAACCTTTCAAGCCTTGACTCAAAATGGTCAAGCGCTTATCGAACACCTATCTCA
AAACGTTAAAACAGAGATCAAATCTGACCTTACGAATAACGTTTTGACGCTGACTTTTGTTGAGCCAAGTAACGAATTAG
ACGAAGACTCACGTTTAAGAGAAGCGTCTTCATTCGATGCACTGCGTTTGGTTCAGCATAGCTTTGAGCAAGACGCGGAT
AACAAACACGCGTTATTCATGGCGGGCCTATTCGCTTACGACATCGTGGCGAACTTTGAACCGCTAGGCGATGCTGAAGC
GACCAACAATTGTCCTGACTTTGTTTTCTACGTTGCTGAAACACTATTACGTTTCGACCACCAAGAAAACGAAGGTCTGC
TTCACGCAAGCTTATTCACTCAAGATGAGAGCATTAAAGCGCAATTAACTGAGCGCCTAGCCGACATTCAAACACAGTGT
CAGTCATTGAAAGCCATCACAGAAGTTACGCCACTCGACAACGTTGACGCTGTACCGAGTGTTTCAGATGAAGACTTCTG
CCAAACGGTTCGTGATTTAAAAGAATACGTCGTTAAAGGCGATGTATTCCAAGTGGTACCTTCACGTCGCTTTACGCTAC
CTTGCCCTGCTCCACTGGCGGCTTACAAAGAACTCAAGCAAAGTAACCCAAGCCCTTACATGTTCTACATGCAAGATGAG
CTGTTTACTCTGTTTGGTGCTTCTCCAGAAAGCGCGCTGAAGTACGAAACTGAAACCAACCAAATCGAGATTTACCCAAT
TGCTGGTACTCGTCGTCGTGGTAAGCGCCCGAATGGGCAAATTGATTTCGACCTAGATAGCCGTATCGAACTTGAACTGC
GTACCGACAAGAAAGAAAACGCTGAACACATGATGCTGGTCGACCTAGCACGTAACGATGTGGCACGTATTGCAGAAGCT
GGCACTCGCCACGTAGCAGACTTGCTGAAAGTTGATCGCTACAGTCATGTGATGCACTTGGTTTCTCGTGTTGTTGGTCA
ACTACGTGAAGACTTAGATGCCCTGCACGCTTACCAAGCTTGTATGAACATGGGTACGCTAACAGGCGCACCAAAAATCC
GTGCAATGCAGCTTATTCGTGACGTAGAAAAAACACGTCGTGGTAGCTACGGCGGTGCAGTTGGTTACCTAACTGGTGAA
GGCACGTTAGATACTTGTATCGTGATTCGCTCTGCTTACGTTGAAGATGGCGTGGCACAAGTACAAGCGGGCGCAGGTGT
GGTATTCGACTCAGACCCACAAGCAGAAGCAGATGAAACTCGCGGCAAAGCTCAAGCGGTCATCTCTGCGATTCAAGCAG
CTCATACTAAAAACCTAACAACTAAGAAGGAGTCGTAA

Upstream 100 bases:

>100_bases
GCTTTCATAGCGGGCTTTTTAATTTGTCGAACATCTCACATTCAACTGACTAACAAATCAACGAATTAACCATATTCTAG
ATTGGGTGGAAGGTATCTTT

Downstream 100 bases:

>100_bases
TCATGGCTGATATTGTATTCATCGATAACTTCGACTCGTTCACTTACAACCTTGTAGACCAGTTCCGTTCATTAGGTCAC
AGCGTGAAAATTTACCGTAA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MRLCTAGNLKNHKGGLVNKAIEIKKLGTIEVINSSVPYSQDPTSVFHTLCENKTDSLLLESAEIESKQNLTSLLLIDSAV
RIVCRGHEVTFQALTQNGQALIEHLSQNVKTEIKSDLTNNVLTLTFVEPSNELDEDSRLREASSFDALRLVQHSFEQDAD
NKHALFMAGLFAYDIVANFEPLGDAEATNNCPDFVFYVAETLLRFDHQENEGLLHASLFTQDESIKAQLTERLADIQTQC
QSLKAITEVTPLDNVDAVPSVSDEDFCQTVRDLKEYVVKGDVFQVVPSRRFTLPCPAPLAAYKELKQSNPSPYMFYMQDE
LFTLFGASPESALKYETETNQIEIYPIAGTRRRGKRPNGQIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARIAEA
GTRHVADLLKVDRYSHVMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGSYGGAVGYLTGE
GTLDTCIVIRSAYVEDGVAQVQAGAGVVFDSDPQAEADETRGKAQAVISAIQAAHTKNLTTKKES

Sequences:

>Translated_545_residues
MRLCTAGNLKNHKGGLVNKAIEIKKLGTIEVINSSVPYSQDPTSVFHTLCENKTDSLLLESAEIESKQNLTSLLLIDSAV
RIVCRGHEVTFQALTQNGQALIEHLSQNVKTEIKSDLTNNVLTLTFVEPSNELDEDSRLREASSFDALRLVQHSFEQDAD
NKHALFMAGLFAYDIVANFEPLGDAEATNNCPDFVFYVAETLLRFDHQENEGLLHASLFTQDESIKAQLTERLADIQTQC
QSLKAITEVTPLDNVDAVPSVSDEDFCQTVRDLKEYVVKGDVFQVVPSRRFTLPCPAPLAAYKELKQSNPSPYMFYMQDE
LFTLFGASPESALKYETETNQIEIYPIAGTRRRGKRPNGQIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARIAEA
GTRHVADLLKVDRYSHVMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGSYGGAVGYLTGE
GTLDTCIVIRSAYVEDGVAQVQAGAGVVFDSDPQAEADETRGKAQAVISAIQAAHTKNLTTKKES
>Mature_545_residues
MRLCTAGNLKNHKGGLVNKAIEIKKLGTIEVINSSVPYSQDPTSVFHTLCENKTDSLLLESAEIESKQNLTSLLLIDSAV
RIVCRGHEVTFQALTQNGQALIEHLSQNVKTEIKSDLTNNVLTLTFVEPSNELDEDSRLREASSFDALRLVQHSFEQDAD
NKHALFMAGLFAYDIVANFEPLGDAEATNNCPDFVFYVAETLLRFDHQENEGLLHASLFTQDESIKAQLTERLADIQTQC
QSLKAITEVTPLDNVDAVPSVSDEDFCQTVRDLKEYVVKGDVFQVVPSRRFTLPCPAPLAAYKELKQSNPSPYMFYMQDE
LFTLFGASPESALKYETETNQIEIYPIAGTRRRGKRPNGQIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARIAEA
GTRHVADLLKVDRYSHVMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGSYGGAVGYLTGE
GTLDTCIVIRSAYVEDGVAQVQAGAGVVFDSDPQAEADETRGKAQAVISAIQAAHTKNLTTKKES

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=514, Percent_Identity=58.7548638132296, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1788114, Length=366, Percent_Identity=31.6939890710383, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1786809, Length=215, Percent_Identity=31.1627906976744, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082077, Length=275, Percent_Identity=27.6363636363636, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=377, Percent_Identity=36.604774535809, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324361, Length=289, Percent_Identity=27.3356401384083, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 60464; Mature: 60464

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLCTAGNLKNHKGGLVNKAIEIKKLGTIEVINSSVPYSQDPTSVFHTLCENKTDSLLLE
CCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHEEH
SAEIESKQNLTSLLLIDSAVRIVCRGHEVTFQALTQNGQALIEHLSQNVKTEIKSDLTNN
HHHHCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
VLTLTFVEPSNELDEDSRLREASSFDALRLVQHSFEQDADNKHALFMAGLFAYDIVANFE
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
PLGDAEATNNCPDFVFYVAETLLRFDHQENEGLLHASLFTQDESIKAQLTERLADIQTQC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QSLKAITEVTPLDNVDAVPSVSDEDFCQTVRDLKEYVVKGDVFQVVPSRRFTLPCPAPLA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCEECCCCCHHH
AYKELKQSNPSPYMFYMQDELFTLFGASPESALKYETETNQIEIYPIAGTRRRGKRPNGQ
HHHHHHHCCCCCEEEEEECCHHHHCCCCCHHHEEEECCCCEEEEEECCCCCCCCCCCCCC
IDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARIAEAGTRHVADLLKVDRYSHVMHL
EEECCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGSYGGAVGYLTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GTLDTCIVIRSAYVEDGVAQVQAGAGVVFDSDPQAEADETRGKAQAVISAIQAAHTKNLT
CCHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TKKES
CCCCC
>Mature Secondary Structure
MRLCTAGNLKNHKGGLVNKAIEIKKLGTIEVINSSVPYSQDPTSVFHTLCENKTDSLLLE
CCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHEEH
SAEIESKQNLTSLLLIDSAVRIVCRGHEVTFQALTQNGQALIEHLSQNVKTEIKSDLTNN
HHHHCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
VLTLTFVEPSNELDEDSRLREASSFDALRLVQHSFEQDADNKHALFMAGLFAYDIVANFE
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
PLGDAEATNNCPDFVFYVAETLLRFDHQENEGLLHASLFTQDESIKAQLTERLADIQTQC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QSLKAITEVTPLDNVDAVPSVSDEDFCQTVRDLKEYVVKGDVFQVVPSRRFTLPCPAPLA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCEECCCCCHHH
AYKELKQSNPSPYMFYMQDELFTLFGASPESALKYETETNQIEIYPIAGTRRRGKRPNGQ
HHHHHHHCCCCCEEEEEECCHHHHCCCCCHHHEEEECCCCEEEEEECCCCCCCCCCCCCC
IDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARIAEAGTRHVADLLKVDRYSHVMHL
EEECCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGSYGGAVGYLTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GTLDTCIVIRSAYVEDGVAQVQAGAGVVFDSDPQAEADETRGKAQAVISAIQAAHTKNLT
CCHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TKKES
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]