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The map label for this gene is rluB [H]

Identifier: 218709944

GI number: 218709944

Start: 2105961

End: 2106989

Strand: Reverse

Name: rluB [H]

Synonym: VS_1962

Alternate gene names: 218709944

Gene position: 2106989-2105961 (Counterclockwise)

Preceding gene: 218709945

Following gene: 218709940

Centisome position: 63.86

GC content: 47.33

Gene sequence:

>1029_bases
ATGAGCGAAAAATTACAGAAGGTTTTAGCGCGAGCTGGTCACGGTTCTCGTCGTGAACTAGAAGGTTTAATCAAATCTGG
TCGTGTAAGTGTTAACGGTCAAGTTGCGAAATTGGGTGAGCGTCTTGAAGACGAGAACTCAGTGATCCGTATCGATGGCC
ACACTATTACAGGCAAAGCCTCTGAAGAAGTGGTTTGTCGTGTACTTGCTTACTACAAACCTGAAGGTGAGCTTTGTACT
CGTCACGATCCTGAAGGTCGCCGTACTGTTTTCGATCGTCTACCTAAGATCCGTGGTTCTCGTTGGATTTCAGTTGGTCG
TCTTGATGCAAACACATCAGGCCTACTGTTGTTCACAACCGATGGTGAACTAGCTAACCGCCTAATGCACCCAAGCCGTC
AGGTTGAGCGTGAGTACCTAGTACGTGTATTTGGTGAAGTAACTGAGCAAAAAGTTAAGAACGTGACTCGTGGCGTTCAA
CTTGAAGATGGTATGGCTCGTTTCGAAGATGTGGTTTACGCTGGCGGTGAAGGTATGAACCATACTTTCTACGTAGCAAT
TAACGAAGGTCGTAACCGTGAGGTTCGTCGTCTTTGGGAATCACAAGAAACAACAGTAAGCCGTCTGAAACGTGTACGTT
ACGGTGATATCTACCTTGATAAGAAACTGCCTCGTGGCGGTTGGAAAGAGCTAGATCTTCAAGAAGTAAACTACTTGCGA
GAGCTTGTTGAACTTCAGCCAGAAAAAGAGACACTGTTGGATCTTGATCCTGCAAACACTTCTCGTAAGCGTGAGCGTTC
TCGTAGCCAAAAAATTCGTCGCGCTGTTAAGCGTCACGAAGAGCGTGCAAATGCTCCTAAAGGCCGTAGCAACCAGACTA
AGCGTAAGAAGCCTGCAACTCGCGGTACTACAACACCTGATTCAGGCCGTAGTGCTCCAGCGACCAATGGCAAACCTCAG
GCTACCAAAAAGCCTAAACTGAACAACGGACGCCCGGCTAAACCGGCTAAGCCAAGATCACGTAAGTAA

Upstream 100 bases:

>100_bases
TGCTTTTTGGCAGGTTATTTGCGATAATACGCGACCGCGATTTGGTCGCGAAAAATTCATTCGACGTCTGTGAAGACGAC
AATTAGGTAGAAAAGAGTAA

Downstream 100 bases:

>100_bases
TTACGTGGATTCTGAAGCTTAATCCAACGTTTCGAATACAAAAAAACCTGCTAACTTAGCAGGTTTTTTTATGTCTTGAA
TATGGCGCAGTTCAAATCAT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSEKLQKVLARAGHGSRRELEGLIKSGRVSVNGQVAKLGERLEDENSVIRIDGHTITGKASEEVVCRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVTEQKVKNVTRGVQ
LEDGMARFEDVVYAGGEGMNHTFYVAINEGRNREVRRLWESQETTVSRLKRVRYGDIYLDKKLPRGGWKELDLQEVNYLR
ELVELQPEKETLLDLDPANTSRKRERSRSQKIRRAVKRHEERANAPKGRSNQTKRKKPATRGTTTPDSGRSAPATNGKPQ
ATKKPKLNNGRPAKPAKPRSRK

Sequences:

>Translated_342_residues
MSEKLQKVLARAGHGSRRELEGLIKSGRVSVNGQVAKLGERLEDENSVIRIDGHTITGKASEEVVCRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVTEQKVKNVTRGVQ
LEDGMARFEDVVYAGGEGMNHTFYVAINEGRNREVRRLWESQETTVSRLKRVRYGDIYLDKKLPRGGWKELDLQEVNYLR
ELVELQPEKETLLDLDPANTSRKRERSRSQKIRRAVKRHEERANAPKGRSNQTKRKKPATRGTTTPDSGRSAPATNGKPQ
ATKKPKLNNGRPAKPAKPRSRK
>Mature_341_residues
SEKLQKVLARAGHGSRRELEGLIKSGRVSVNGQVAKLGERLEDENSVIRIDGHTITGKASEEVVCRVLAYYKPEGELCTR
HDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVTEQKVKNVTRGVQL
EDGMARFEDVVYAGGEGMNHTFYVAINEGRNREVRRLWESQETTVSRLKRVRYGDIYLDKKLPRGGWKELDLQEVNYLRE
LVELQPEKETLLDLDPANTSRKRERSRSQKIRRAVKRHEERANAPKGRSNQTKRKKPATRGTTTPDSGRSAPATNGKPQA
TKKPKLNNGRPAKPAKPRSRK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=294, Percent_Identity=64.6258503401361, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=29.2181069958848, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=29.2372881355932, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081838, Length=206, Percent_Identity=29.126213592233, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 38896; Mature: 38765

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRRELEGLIKSGRVSVNGQVAKLGERLEDENSVIRIDGHTITGKA
CCHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHCCCCCEEEECCEEECCCC
SEEVVCRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVTEQKVKNVTRGVQLEDGMARFEDVVYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCC
HTFYVAINEGRNREVRRLWESQETTVSRLKRVRYGDIYLDKKLPRGGWKELDLQEVNYLR
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHH
ELVELQPEKETLLDLDPANTSRKRERSRSQKIRRAVKRHEERANAPKGRSNQTKRKKPAT
HHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
RGTTTPDSGRSAPATNGKPQATKKPKLNNGRPAKPAKPRSRK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRRELEGLIKSGRVSVNGQVAKLGERLEDENSVIRIDGHTITGKA
CHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHCCCCCEEEECCEEECCCC
SEEVVCRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVTEQKVKNVTRGVQLEDGMARFEDVVYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCC
HTFYVAINEGRNREVRRLWESQETTVSRLKRVRYGDIYLDKKLPRGGWKELDLQEVNYLR
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHH
ELVELQPEKETLLDLDPANTSRKRERSRSQKIRRAVKRHEERANAPKGRSNQTKRKKPAT
HHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
RGTTTPDSGRSAPATNGKPQATKKPKLNNGRPAKPAKPRSRK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]