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The map label for this gene is polB [H]

Identifier: 218709939

GI number: 218709939

Start: 2100907

End: 2103270

Strand: Direct

Name: polB [H]

Synonym: VS_1957

Alternate gene names: 218709939

Gene position: 2100907-2103270 (Clockwise)

Preceding gene: 218709931

Following gene: 218709941

Centisome position: 63.68

GC content: 45.39

Gene sequence:

>2364_bases
TTGGATATTCAGCAAGGCTTTGTACTCACAAGACAAGCAAGAGACTTTTCAGGGCGCACACAAATCGACTTGTGGTTAAG
CACCCCTCAAGGCCCTACGCTACTGACTATTCAAAATGAAAAGCCTGTATTTTTTGTCGCGCAAACTGATGTTGAAGCAT
GTCAGGCTATCGCAGCGAAAGAGTCTATCAATTGTCAGTTTAAGCCTTTAGAGTTGGCGACATTTGACCAAACCCCTTTA
GCCGCTTGTTACACCTCTCTGTCGAGAAGTAGCTTTGGTTTAGCGCAAGCCTTTCACAGCGAAGAAATTCAGATCTTTGA
AAGCGATATTCGACTCGCCGATCGATATCTGATGGAACGCTTTATCAAGGGCAGTATCGAATTCACAGGCACTATCACTC
CAAAAAGACAGCATCGCAGAGTATCGAACGCAAAATGCCGAGCTGGCGATTACTTGCCAAACTTGTCTGTGGTTTCGCTT
GATATTGAGTGTTCGGAAAAAGGTGTGCTTTATTCCATCGGCCTAGACAGTCCAATGGATAGCCGAGTGATAATGATTGG
CGATCCACAAGAAGCAGACACCAATATCCAATGGGTTGCTAACGAGAAAGCGCTGCTTGAAGCTATGATTGCGTGGTTCT
CTGAGTTTGACCCAGACATCATCATCGGTTGGAATGTCATTGATTTCGACTTTAGGCTGCTGCACAAACGTTCAGAATGG
AACGAAGTGAAACTCAGTATTGGTCGCGACAATCAACCGAGCTTCTTTCGCAGCTCCGCACAAAACCAGCAGGGATTCAT
TACCATTCCTGGTCGCGTGGTCTTAGATGGTATCGATACGCTTAAAACAGCAACCTACCATTTCAGGTCATGGTCTCTTG
AGTCCGTGTCACAAGAGTTACTGGGCGAAGGCAAAGACATTCATAATGTTCATGACCGCATGGATGAAATCAATCGAATG
TTTAAGTTCGATAAACCTTCACTTGCCAAGTACAACCTTCAAGACTGTGTGCTAGTAAACCGAATCTTCGAACACACTCA
CCTACTCGATTTTGCTATTGAACGTTCTCGCTTAACTGGTGTTGAACTTGATCGCGTTGGCGGCTCTGTCGCTGCTTTTA
CTAACTTATACATGCCTCAGATACACCGAGCGGGTTACGTCGCGCCGAACTTAGAGCCTGAGAACTGGATCGCTAGCCCC
GGTGGTTATGTGATGGATTCGATTCCCAACCTTTATGACTCTGTGTTGGTACTCGATTTTAAGAGCCTTTACCCATCGAT
CATTCGTTCTTTCTTAATTGACCCGATGGGGTTAATTGAAGGACTAAAGTTAGAGTTAGGCTCTGAAGAAGACCAAGCGG
TGGCTGGGTTTCGTGGCGGTCAATTCCACCGCTCTAAGCACTTTCTACCAGAGATGATCGAAAATCTCTGGGCAGCGCGT
GATGTCGCAAAGAAAAATAACGAGAAAGCTTTTTCTCAGGCGATTAAGATCATCATGAACTCTTTCTATGGTGTGCTGGG
TTCTTCTGGTTGTCGTTTCTTTGATACTCGATTGGCGTCATCGATCACCATGCGTGGTCATGAGATCATGAAGCAAACCA
AGGTTCTGATCGAAGATAAAGGGTATCAAGTAATTTACGGAGATACCGATTCAACTTTCGTGTCTCTTAATGGCAGTTTT
GAGCAAGAACAAGCAGACGAGATTGGTCACTCTCTTGTGGCTTACATCAACGACTGGTGGACGAATCACCTGAAAGAGGT
CTACAACCTCACCTCGATACTTGAATTGGAATACGAGACCCATTACCGCAAGTTTCTAATGCCGACAATTCGGGGCTCTG
AGACAGGATCGAAGAAACGTTATGCAGGCTTAATCAATCAAGGTGACCAAGAGAAGATCATTTTTAAAGGCCTTGAAAGT
GCACGAACCGATTGGACGCCTCTCGCACAACAGTTCCAACAAACCTTGTATGAGATGGTCTTTCACGACCAAGATCCAAG
TGATTATGTTAGACAGTTTGTCGATGAAACATCCGCAGGCAAACACGATGACCTACTGGTTTATCAAAAGCGCTTGCGCC
GTAAATTGCATGAATACCAGAAGAACATTCCGCCACAGGTTCGCGCAGCAAGATTAGCCGATGAGATCAATGCTCAGCTC
GGTCGCCCACTTCAATACCAAAACAAAGGACGTATTGAATATTTGATTACGCTGAGTGGCCCTGAGCCCAAGGAGTACTT
AAAGAGTGGCATCGATTATCAACATTACATCGACAAGCAGATTAAACCGGTCGCAGAAGCGATTTTGCCCTTCATTGGTT
TAGATTTTGAAAGAGTTAGCGGGCAGCAACTAGGCCTGTTCTAG

Upstream 100 bases:

>100_bases
CCTCTGTTAACTAGAATTTAGTTAGTATTTTTGCTGCCTGTTATGCGCTGAGGGTCGCTAGGTGGTAGTATTCGATTCCC
AATTTCAATAGGTTACTACA

Downstream 100 bases:

>100_bases
ATTTAATTCTCTTTTCGAGGCGACATGAGACTCGAGATATCTATTAAAACTCGAACCGGACATCAAAACGAAAAAAGCAG
AGGAGTTCTTAACTCGCTCT

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MDIQQGFVLTRQARDFSGRTQIDLWLSTPQGPTLLTIQNEKPVFFVAQTDVEACQAIAAKESINCQFKPLELATFDQTPL
AACYTSLSRSSFGLAQAFHSEEIQIFESDIRLADRYLMERFIKGSIEFTGTITPKRQHRRVSNAKCRAGDYLPNLSVVSL
DIECSEKGVLYSIGLDSPMDSRVIMIGDPQEADTNIQWVANEKALLEAMIAWFSEFDPDIIIGWNVIDFDFRLLHKRSEW
NEVKLSIGRDNQPSFFRSSAQNQQGFITIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKDIHNVHDRMDEINRM
FKFDKPSLAKYNLQDCVLVNRIFEHTHLLDFAIERSRLTGVELDRVGGSVAAFTNLYMPQIHRAGYVAPNLEPENWIASP
GGYVMDSIPNLYDSVLVLDFKSLYPSIIRSFLIDPMGLIEGLKLELGSEEDQAVAGFRGGQFHRSKHFLPEMIENLWAAR
DVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDTRLASSITMRGHEIMKQTKVLIEDKGYQVIYGDTDSTFVSLNGSF
EQEQADEIGHSLVAYINDWWTNHLKEVYNLTSILELEYETHYRKFLMPTIRGSETGSKKRYAGLINQGDQEKIIFKGLES
ARTDWTPLAQQFQQTLYEMVFHDQDPSDYVRQFVDETSAGKHDDLLVYQKRLRRKLHEYQKNIPPQVRAARLADEINAQL
GRPLQYQNKGRIEYLITLSGPEPKEYLKSGIDYQHYIDKQIKPVAEAILPFIGLDFERVSGQQLGLF

Sequences:

>Translated_787_residues
MDIQQGFVLTRQARDFSGRTQIDLWLSTPQGPTLLTIQNEKPVFFVAQTDVEACQAIAAKESINCQFKPLELATFDQTPL
AACYTSLSRSSFGLAQAFHSEEIQIFESDIRLADRYLMERFIKGSIEFTGTITPKRQHRRVSNAKCRAGDYLPNLSVVSL
DIECSEKGVLYSIGLDSPMDSRVIMIGDPQEADTNIQWVANEKALLEAMIAWFSEFDPDIIIGWNVIDFDFRLLHKRSEW
NEVKLSIGRDNQPSFFRSSAQNQQGFITIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKDIHNVHDRMDEINRM
FKFDKPSLAKYNLQDCVLVNRIFEHTHLLDFAIERSRLTGVELDRVGGSVAAFTNLYMPQIHRAGYVAPNLEPENWIASP
GGYVMDSIPNLYDSVLVLDFKSLYPSIIRSFLIDPMGLIEGLKLELGSEEDQAVAGFRGGQFHRSKHFLPEMIENLWAAR
DVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDTRLASSITMRGHEIMKQTKVLIEDKGYQVIYGDTDSTFVSLNGSF
EQEQADEIGHSLVAYINDWWTNHLKEVYNLTSILELEYETHYRKFLMPTIRGSETGSKKRYAGLINQGDQEKIIFKGLES
ARTDWTPLAQQFQQTLYEMVFHDQDPSDYVRQFVDETSAGKHDDLLVYQKRLRRKLHEYQKNIPPQVRAARLADEINAQL
GRPLQYQNKGRIEYLITLSGPEPKEYLKSGIDYQHYIDKQIKPVAEAILPFIGLDFERVSGQQLGLF
>Mature_787_residues
MDIQQGFVLTRQARDFSGRTQIDLWLSTPQGPTLLTIQNEKPVFFVAQTDVEACQAIAAKESINCQFKPLELATFDQTPL
AACYTSLSRSSFGLAQAFHSEEIQIFESDIRLADRYLMERFIKGSIEFTGTITPKRQHRRVSNAKCRAGDYLPNLSVVSL
DIECSEKGVLYSIGLDSPMDSRVIMIGDPQEADTNIQWVANEKALLEAMIAWFSEFDPDIIIGWNVIDFDFRLLHKRSEW
NEVKLSIGRDNQPSFFRSSAQNQQGFITIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKDIHNVHDRMDEINRM
FKFDKPSLAKYNLQDCVLVNRIFEHTHLLDFAIERSRLTGVELDRVGGSVAAFTNLYMPQIHRAGYVAPNLEPENWIASP
GGYVMDSIPNLYDSVLVLDFKSLYPSIIRSFLIDPMGLIEGLKLELGSEEDQAVAGFRGGQFHRSKHFLPEMIENLWAAR
DVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDTRLASSITMRGHEIMKQTKVLIEDKGYQVIYGDTDSTFVSLNGSF
EQEQADEIGHSLVAYINDWWTNHLKEVYNLTSILELEYETHYRKFLMPTIRGSETGSKKRYAGLINQGDQEKIIFKGLES
ARTDWTPLAQQFQQTLYEMVFHDQDPSDYVRQFVDETSAGKHDDLLVYQKRLRRKLHEYQKNIPPQVRAARLADEINAQL
GRPLQYQNKGRIEYLITLSGPEPKEYLKSGIDYQHYIDKQIKPVAEAILPFIGLDFERVSGQQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=710, Percent_Identity=26.7605633802817, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI106507301, Length=795, Percent_Identity=22.1383647798742, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1786246, Length=790, Percent_Identity=51.3924050632911, Blast_Score=806, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=709, Percent_Identity=26.2341325811001, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32565317, Length=660, Percent_Identity=26.5151515151515, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320101, Length=630, Percent_Identity=26.1904761904762, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324227, Length=614, Percent_Identity=25.0814332247557, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325090, Length=434, Percent_Identity=25.3456221198157, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24664937, Length=710, Percent_Identity=25.0704225352113, Blast_Score=142, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24648780, Length=652, Percent_Identity=23.0061349693252, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 89853; Mature: 89853

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQQGFVLTRQARDFSGRTQIDLWLSTPQGPTLLTIQNEKPVFFVAQTDVEACQAIAAK
CCCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHHH
ESINCQFKPLELATFDQTPLAACYTSLSRSSFGLAQAFHSEEIQIFESDIRLADRYLMER
HCCCCEEECEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FIKGSIEFTGTITPKRQHRRVSNAKCRAGDYLPNLSVVSLDIECSEKGVLYSIGLDSPMD
HHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCC
SRVIMIGDPQEADTNIQWVANEKALLEAMIAWFSEFDPDIIIGWNVIDFDFRLLHKRSEW
CEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECEEECHHHHHHHCCCCC
NEVKLSIGRDNQPSFFRSSAQNQQGFITIPGRVVLDGIDTLKTATYHFRSWSLESVSQEL
CEEEEEECCCCCCHHHHHCCCCCCCEEEECCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
LGEGKDIHNVHDRMDEINRMFKFDKPSLAKYNLQDCVLVNRIFEHTHLLDFAIERSRLTG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VELDRVGGSVAAFTNLYMPQIHRAGYVAPNLEPENWIASPGGYVMDSIPNLYDSVLVLDF
CCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KSLYPSIIRSFLIDPMGLIEGLKLELGSEEDQAVAGFRGGQFHRSKHFLPEMIENLWAAR
HHHHHHHHHHHHCCHHHHHCCCCEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDTRLASSITMRGHEIMKQTKVLIEDK
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC
GYQVIYGDTDSTFVSLNGSFEQEQADEIGHSLVAYINDWWTNHLKEVYNLTSILELEYET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHH
HYRKFLMPTIRGSETGSKKRYAGLINQGDQEKIIFKGLESARTDWTPLAQQFQQTLYEMV
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDQDPSDYVRQFVDETSAGKHDDLLVYQKRLRRKLHEYQKNIPPQVRAARLADEINAQL
HCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRPLQYQNKGRIEYLITLSGPEPKEYLKSGIDYQHYIDKQIKPVAEAILPFIGLDFERVS
CCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
GQQLGLF
CCCCCCC
>Mature Secondary Structure
MDIQQGFVLTRQARDFSGRTQIDLWLSTPQGPTLLTIQNEKPVFFVAQTDVEACQAIAAK
CCCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHHH
ESINCQFKPLELATFDQTPLAACYTSLSRSSFGLAQAFHSEEIQIFESDIRLADRYLMER
HCCCCEEECEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FIKGSIEFTGTITPKRQHRRVSNAKCRAGDYLPNLSVVSLDIECSEKGVLYSIGLDSPMD
HHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCC
SRVIMIGDPQEADTNIQWVANEKALLEAMIAWFSEFDPDIIIGWNVIDFDFRLLHKRSEW
CEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECEEECHHHHHHHCCCCC
NEVKLSIGRDNQPSFFRSSAQNQQGFITIPGRVVLDGIDTLKTATYHFRSWSLESVSQEL
CEEEEEECCCCCCHHHHHCCCCCCCEEEECCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
LGEGKDIHNVHDRMDEINRMFKFDKPSLAKYNLQDCVLVNRIFEHTHLLDFAIERSRLTG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VELDRVGGSVAAFTNLYMPQIHRAGYVAPNLEPENWIASPGGYVMDSIPNLYDSVLVLDF
CCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KSLYPSIIRSFLIDPMGLIEGLKLELGSEEDQAVAGFRGGQFHRSKHFLPEMIENLWAAR
HHHHHHHHHHHHCCHHHHHCCCCEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDTRLASSITMRGHEIMKQTKVLIEDK
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC
GYQVIYGDTDSTFVSLNGSFEQEQADEIGHSLVAYINDWWTNHLKEVYNLTSILELEYET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHH
HYRKFLMPTIRGSETGSKKRYAGLINQGDQEKIIFKGLESARTDWTPLAQQFQQTLYEMV
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDQDPSDYVRQFVDETSAGKHDDLLVYQKRLRRKLHEYQKNIPPQVRAARLADEINAQL
HCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRPLQYQNKGRIEYLITLSGPEPKEYLKSGIDYQHYIDKQIKPVAEAILPFIGLDFERVS
CCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
GQQLGLF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]