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The map label for this gene is nrfA [H]

Identifier: 218709920

GI number: 218709920

Start: 2075990

End: 2077378

Strand: Direct

Name: nrfA [H]

Synonym: VS_1933

Alternate gene names: 218709920

Gene position: 2075990-2077378 (Clockwise)

Preceding gene: 218709903

Following gene: 218709921

Centisome position: 62.92

GC content: 48.24

Gene sequence:

>1389_bases
ATGGTTAGCGCATCACTAGCAAGCGCGACCAGTTTTGCTGCGTCTGAACAAAAAGAAATTGGCGACCCTCGTAACGACCA
GTTCGAGCAAAACCACCCAGATCAATACCATTCATGGAGACAAACTTCAGAAAGCGAAGTTATTGAAGACGCACTAAAAG
AAGATCCAAACATGGTCATCATGTGGGCTGGCTACGGCTTCGCAAAAGATTACAACAAAGCACGCGGCCACTTTTATGCC
ATTGACGATGTAAGACAAACACTTCGTACTGGCGGCCCAACGGACGAAAGCTCTGGCCCAATGCCAATGGCGTGTTGGAG
CTGTAAGAGCCCAGATGTCGCTCGTGTTATCGAAGAGCGTGGTGAAGATGGTTATTTTGAAGGTAAATGGGCGCGTCTTG
GCAACGAGATCATTAACCCTATTGGCTGTTCAGACTGTCACGACACCCAAAGCGAAGGCTTTAAGAATGGTGAGCCTGCA
CTGAAGGTGACTCGTCCTTACGTTGAGCGTGCATTTGAAACGATTGGTAAAAAGTTTGATGAACAAAGTCGTTTAGACCA
ACAAGCTTCTGTTTGTGCTCAGTGCCACGTGGAATACTACTTCACAGGGCCAACCAAAGGTGTGAAATTCCCTTGGGACC
AAGGTACAACCGTTGGCGACATGGAACGTTACTATGATGCAATCAACTTTAAAGATTGGACGCATAAAGTATCGAAAGCA
CCCATGCTGAAAGCACAGCACCCAGGCTTTGAAACATGGCGCGAAGGCATCCACGGCAAGAACAAAGTCGTTTGTGCTGA
CTGTCATATGCCGAAAGTCACCAAAGAAGATGGCACTGTTTATACCGACCATAAAGTGGGTAACCCATTCGACCGCTTCG
AAGACACGTGTGCAAACTGTCACACTCAATCGAAAGAAACCATGCGTAACATCGTTTCAAGCCGTAAAGCGCAAGTACTG
AACATGAAGCTGACAGCAGAGAAACAAATCGTTGCTGCTCACTTTGAAGCAGGCGCAGCATGGGAAGCGGGTGCGACAGA
GCAAGAGATGGAACCTATCCTACTCGATATCCGTCACGCTCAATGGCGCTGGGACTACGCTATCGCGTCTCACGGTATTC
ACATGCACGCACCTGAGATCGCTCTAGAAGTGCTAGGTACTGCCGTTGACCGCGCAGCCGATGCTCGTACTAAGATTGTT
CGTCTACTGGCGAAGAAAGGCATCACCGATCCAATCGAGATTCCAGATATCTCGACAAAAGAAGCGGCTCAAAAAGCGCT
TGGAATGGACATGGATAAGATGAACGCAGAGAAACAGCACTTCTTAGACACGGTTGTTCCTAAATGGGAAGAGCAAGCTG
AAAAGCGTGAAGCCAACTACGAATACTAG

Upstream 100 bases:

>100_bases
ATTTGAATAGCAAACTCACAACGCTTGGTATAAGAATCAGTATATTGGAGATATCACTGTGAAAAAGCATTGGATACGTA
ATTCGGTCACAGCACTATTA

Downstream 100 bases:

>100_bases
TTTCTTCCATTAGACCATTAAGTAAAAACCAGTCACACTATTGTTGTGTGGCTGGTTTTTTTAGGTTTTAGGGGAATACA
GATCAATATCGAATCCCCGC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MVSASLASATSFAASEQKEIGDPRNDQFEQNHPDQYHSWRQTSESEVIEDALKEDPNMVIMWAGYGFAKDYNKARGHFYA
IDDVRQTLRTGGPTDESSGPMPMACWSCKSPDVARVIEERGEDGYFEGKWARLGNEIINPIGCSDCHDTQSEGFKNGEPA
LKVTRPYVERAFETIGKKFDEQSRLDQQASVCAQCHVEYYFTGPTKGVKFPWDQGTTVGDMERYYDAINFKDWTHKVSKA
PMLKAQHPGFETWREGIHGKNKVVCADCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKETMRNIVSSRKAQVL
NMKLTAEKQIVAAHFEAGAAWEAGATEQEMEPILLDIRHAQWRWDYAIASHGIHMHAPEIALEVLGTAVDRAADARTKIV
RLLAKKGITDPIEIPDISTKEAAQKALGMDMDKMNAEKQHFLDTVVPKWEEQAEKREANYEY

Sequences:

>Translated_462_residues
MVSASLASATSFAASEQKEIGDPRNDQFEQNHPDQYHSWRQTSESEVIEDALKEDPNMVIMWAGYGFAKDYNKARGHFYA
IDDVRQTLRTGGPTDESSGPMPMACWSCKSPDVARVIEERGEDGYFEGKWARLGNEIINPIGCSDCHDTQSEGFKNGEPA
LKVTRPYVERAFETIGKKFDEQSRLDQQASVCAQCHVEYYFTGPTKGVKFPWDQGTTVGDMERYYDAINFKDWTHKVSKA
PMLKAQHPGFETWREGIHGKNKVVCADCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKETMRNIVSSRKAQVL
NMKLTAEKQIVAAHFEAGAAWEAGATEQEMEPILLDIRHAQWRWDYAIASHGIHMHAPEIALEVLGTAVDRAADARTKIV
RLLAKKGITDPIEIPDISTKEAAQKALGMDMDKMNAEKQHFLDTVVPKWEEQAEKREANYEY
>Mature_462_residues
MVSASLASATSFAASEQKEIGDPRNDQFEQNHPDQYHSWRQTSESEVIEDALKEDPNMVIMWAGYGFAKDYNKARGHFYA
IDDVRQTLRTGGPTDESSGPMPMACWSCKSPDVARVIEERGEDGYFEGKWARLGNEIINPIGCSDCHDTQSEGFKNGEPA
LKVTRPYVERAFETIGKKFDEQSRLDQQASVCAQCHVEYYFTGPTKGVKFPWDQGTTVGDMERYYDAINFKDWTHKVSKA
PMLKAQHPGFETWREGIHGKNKVVCADCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANCHTQSKETMRNIVSSRKAQVL
NMKLTAEKQIVAAHFEAGAAWEAGATEQEMEPILLDIRHAQWRWDYAIASHGIHMHAPEIALEVLGTAVDRAADARTKIV
RLLAKKGITDPIEIPDISTKEAAQKALGMDMDKMNAEKQHFLDTVVPKWEEQAEKREANYEY

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=432, Percent_Identity=63.1944444444444, Blast_Score=608, Evalue=1e-175,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 52166; Mature: 52166

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSASLASATSFAASEQKEIGDPRNDQFEQNHPDQYHSWRQTSESEVIEDALKEDPNMVI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEE
MWAGYGFAKDYNKARGHFYAIDDVRQTLRTGGPTDESSGPMPMACWSCKSPDVARVIEER
EECCCCCHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHC
GEDGYFEGKWARLGNEIINPIGCSDCHDTQSEGFKNGEPALKVTRPYVERAFETIGKKFD
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
EQSRLDQQASVCAQCHVEYYFTGPTKGVKFPWDQGTTVGDMERYYDAINFKDWTHKVSKA
HHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
PMLKAQHPGFETWREGIHGKNKVVCADCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANC
CCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
HTQSKETMRNIVSSRKAQVLNMKLTAEKQIVAAHFEAGAAWEAGATEQEMEPILLDIRHA
CCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHH
QWRWDYAIASHGIHMHAPEIALEVLGTAVDRAADARTKIVRLLAKKGITDPIEIPDISTK
HEEEHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAAQKALGMDMDKMNAEKQHFLDTVVPKWEEQAEKREANYEY
HHHHHHHCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSASLASATSFAASEQKEIGDPRNDQFEQNHPDQYHSWRQTSESEVIEDALKEDPNMVI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEE
MWAGYGFAKDYNKARGHFYAIDDVRQTLRTGGPTDESSGPMPMACWSCKSPDVARVIEER
EECCCCCHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHC
GEDGYFEGKWARLGNEIINPIGCSDCHDTQSEGFKNGEPALKVTRPYVERAFETIGKKFD
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
EQSRLDQQASVCAQCHVEYYFTGPTKGVKFPWDQGTTVGDMERYYDAINFKDWTHKVSKA
HHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
PMLKAQHPGFETWREGIHGKNKVVCADCHMPKVTKEDGTVYTDHKVGNPFDRFEDTCANC
CCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
HTQSKETMRNIVSSRKAQVLNMKLTAEKQIVAAHFEAGAAWEAGATEQEMEPILLDIRHA
CCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHH
QWRWDYAIASHGIHMHAPEIALEVLGTAVDRAADARTKIVRLLAKKGITDPIEIPDISTK
HEEEHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAAQKALGMDMDKMNAEKQHFLDTVVPKWEEQAEKREANYEY
HHHHHHHCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]