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The map label for this gene is nrfB [H]

Identifier: 218709919

GI number: 218709919

Start: 2074882

End: 2075466

Strand: Reverse

Name: nrfB [H]

Synonym: VS_1932

Alternate gene names: 218709919

Gene position: 2075466-2074882 (Counterclockwise)

Preceding gene: 218709922

Following gene: 218709918

Centisome position: 62.91

GC content: 43.25

Gene sequence:

>585_bases
ATGGGCAATATTAAATTGGCCATAGTCATAATGCTTAAATCCCTTCTAGCATTCTGCCTCTATGGTTATTCCATTCATGC
TGTTGCTGAGCCTGCTGTTACGCCAGTAGGAGAATCAACAAGACATGAAGTCGAATTAATCCGCGACAAAGACTACAAGT
GTGTTCAATGCCATAAAGATTCAAAAGAAACGGTATTGGGTTCACATGGTGAAAGTGCACACGCTTTACTTGGCCGTGAA
GTAAATTGTACCGATTGTCATAGTTCAATTAGCCCCGATCACCGAGAAGGTGCACCTGAAGTGGTGAAGTATCGCGCGGC
TCAATCACAACCAGGAACCGAAAAGGTTTTCCTCGATCCTAGCTTAATTTTAGATGCCAATAGCCAATGTATCGATTGTC
ACAAACCTGATGATCTGCGTGAAGCTAGCTGGACTCACGATGTTCACGCGCAAAACTTAACGTGTTCAAACTGTCATGAT
GTTCATGCGACTGAAGCAAAGGTGTTGGGCTTAGATAAAAAGCAAACCATTAAGCTGTGTGTGGATTGCCATTCAGACTT
CAACCAGAAGAAAGAAGGGGAGTAA

Upstream 100 bases:

>100_bases
TATCATTTAAGCGGTAATAATATCGTTTAAGGGTACTTTTGTTTAACCGGAATATTATTTAGTTGAGAGTTAAATAATAC
CGGTATAAGGAAAGGATAAA

Downstream 100 bases:

>100_bases
TCTATGAGCTGCTCAAGAAGAAACTTTTTAGCAGGCGCTGGTGCCGTTATTTTTACCACGGGTGTAGCGGGAACTGCGGC
GGTGACAAGTCGTAAAACGT

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MGNIKLAIVIMLKSLLAFCLYGYSIHAVAEPAVTPVGESTRHEVELIRDKDYKCVQCHKDSKETVLGSHGESAHALLGRE
VNCTDCHSSISPDHREGAPEVVKYRAAQSQPGTEKVFLDPSLILDANSQCIDCHKPDDLREASWTHDVHAQNLTCSNCHD
VHATEAKVLGLDKKQTIKLCVDCHSDFNQKKEGE

Sequences:

>Translated_194_residues
MGNIKLAIVIMLKSLLAFCLYGYSIHAVAEPAVTPVGESTRHEVELIRDKDYKCVQCHKDSKETVLGSHGESAHALLGRE
VNCTDCHSSISPDHREGAPEVVKYRAAQSQPGTEKVFLDPSLILDANSQCIDCHKPDDLREASWTHDVHAQNLTCSNCHD
VHATEAKVLGLDKKQTIKLCVDCHSDFNQKKEGE
>Mature_193_residues
GNIKLAIVIMLKSLLAFCLYGYSIHAVAEPAVTPVGESTRHEVELIRDKDYKCVQCHKDSKETVLGSHGESAHALLGREV
NCTDCHSSISPDHREGAPEVVKYRAAQSQPGTEKVFLDPSLILDANSQCIDCHKPDDLREASWTHDVHAQNLTCSNCHDV
HATEAKVLGLDKKQTIKLCVDCHSDFNQKKEGE

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=179, Percent_Identity=34.0782122905028, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21415; Mature: 21284

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNIKLAIVIMLKSLLAFCLYGYSIHAVAEPAVTPVGESTRHEVELIRDKDYKCVQCHKD
CCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHEECCCCCEEEEECCC
SKETVLGSHGESAHALLGREVNCTDCHSSISPDHREGAPEVVKYRAAQSQPGTEKVFLDP
CCCCEECCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECC
SLILDANSQCIDCHKPDDLREASWTHDVHAQNLTCSNCHDVHATEAKVLGLDKKQTIKLC
EEEEECCCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHE
VDCHSDFNQKKEGE
EEHHHCCCCCCCCC
>Mature Secondary Structure 
GNIKLAIVIMLKSLLAFCLYGYSIHAVAEPAVTPVGESTRHEVELIRDKDYKCVQCHKD
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHEECCCCCEEEEECCC
SKETVLGSHGESAHALLGREVNCTDCHSSISPDHREGAPEVVKYRAAQSQPGTEKVFLDP
CCCCEECCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECC
SLILDANSQCIDCHKPDDLREASWTHDVHAQNLTCSNCHDVHATEAKVLGLDKKQTIKLC
EEEEECCCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHE
VDCHSDFNQKKEGE
EEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]