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The map label for this gene is insF1 [H]

Identifier: 218709860

GI number: 218709860

Start: 2019544

End: 2020431

Strand: Reverse

Name: insF1 [H]

Synonym: VS_1873

Alternate gene names: 218709860

Gene position: 2020431-2019544 (Counterclockwise)

Preceding gene: 218709861

Following gene: 218709859

Centisome position: 61.24

GC content: 42.57

Gene sequence:

>888_bases
GTGGCAACGATTCGTTGCCCAAGAAAATCGACAAAATTCCGATTCATAGCCCATTACAAAACGCGTTATTCCATTGTCTT
GATGTGTCGTTTTCTATCTGTTTCTAAGTCGGGTTACTACGCATGGCTTGATAGAGAGCCAAGCCGCTACGATCAAGAAG
AGCAGGCTTTGAAGAAGCGCATAATTGAAGTGTTTACCCAGAGTCGAGAGACTTACGGTAGTCCACGAGTTCATGCAGAG
CTGAGGCGCCAAGGAGTTTTGGTTAGCCGCAAGCGTGTAGCTCGGATCATGAGAGAGCAAGGGCTAAGAGCGCGAAGTTA
TCGCATTTACATGAAAATGGCCAAGCTACATCGGTTCTATCAATCGATTAAGAATATTAAAAAAGACACACCAAAACCAA
CGGCAGTTAATCAACAATGGTCTGGAGATCTAACGTATATAAAGCAAGGTAAACGTTGGATGTATCTAGCGGTCGTTATC
GATCTTTACTCGCGTAAAATCGTCGGTTGGTCGCTTGGTAGTAAGAAGAGTACACAACTAACGATGAGTTCGTTGAGAAT
GGCAATTAGAAATCGAAAGCCACAAGAGCGATTATTATTTCATACCGACAGAGGCTCTGAATATCGAGCCCATGAAGTTC
AAGCTTTATTATCAAAAAATGGAATTGTCCCAAGTATGAATCGTCCAGGTCATTGCACTGATAATGCGGAGGTGGAGTCG
TTCTTCCATACGCTCAAAGGCGACATAATTAGGAAAAATAGTTTTAAAAGTGAGAAGCAGCTTAGAGATAAACTTGCCGG
TTATATCCAGCATTTTTATAACCGTTATAGACTGCACTCAAGTCTTGGATATCGAACCCCGCATGAATATGAAGTAGCGA
CGGGTTAA

Upstream 100 bases:

>100_bases
AAAGGACTCAGATCCCGAGCCAGAAAACTGAGTCTAGTCGGATTGCAGAGCTTGAACGTCAGAACGAACAACTTAAGCTC
GAGAATGACTTGCTAAAAAA

Downstream 100 bases:

>100_bases
AGATAATAGGGTGTCCATTTTATCGGGTGAAGATCATTCCCAACGCATGGCATTTGTCATTCCATCGTTGGGTTTTGTGT
TTACGGTGGTATGGTTAAGT

Product: ISVisp1 transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKRIIEVFTQSRETYGSPRVHAE
LRRQGVLVSRKRVARIMREQGLRARSYRIYMKMAKLHRFYQSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVI
DLYSRKIVGWSLGSKKSTQLTMSSLRMAIRNRKPQERLLFHTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVES
FFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG

Sequences:

>Translated_295_residues
MATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKRIIEVFTQSRETYGSPRVHAE
LRRQGVLVSRKRVARIMREQGLRARSYRIYMKMAKLHRFYQSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVI
DLYSRKIVGWSLGSKKSTQLTMSSLRMAIRNRKPQERLLFHTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVES
FFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG
>Mature_294_residues
ATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKRIIEVFTQSRETYGSPRVHAEL
RRQGVLVSRKRVARIMREQGLRARSYRIYMKMAKLHRFYQSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVID
LYSRKIVGWSLGSKKSTQLTMSSLRMAIRNRKPQERLLFHTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVESF
FHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=255, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788405, Length=282, Percent_Identity=38.2978723404255, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1787263, Length=282, Percent_Identity=38.2978723404255, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1786753, Length=282, Percent_Identity=38.2978723404255, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1786570, Length=282, Percent_Identity=38.2978723404255, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1786490, Length=282, Percent_Identity=38.2978723404255, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1789981, Length=281, Percent_Identity=31.6725978647687, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790724, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789424, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789224, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788305, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787669, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786558, Length=286, Percent_Identity=26.5734265734266, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 34889; Mature: 34758

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKR
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHH
IIEVFTQSRETYGSPRVHAELRRQGVLVSRKRVARIMREQGLRARSYRIYMKMAKLHRFY
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVIDLYSRKIVGWSLGSKKSTQL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHH
TMSSLRMAIRNRKPQERLLFHTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVES
HHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
FFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG
HHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
>Mature Secondary Structure 
ATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKR
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHH
IIEVFTQSRETYGSPRVHAELRRQGVLVSRKRVARIMREQGLRARSYRIYMKMAKLHRFY
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVIDLYSRKIVGWSLGSKKSTQL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHH
TMSSLRMAIRNRKPQERLLFHTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVES
HHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
FFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG
HHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]