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The map label for this gene is adh1 [H]

Identifier: 218709823

GI number: 218709823

Start: 1976329

End: 1977621

Strand: Reverse

Name: adh1 [H]

Synonym: VS_1835

Alternate gene names: 218709823

Gene position: 1977621-1976329 (Counterclockwise)

Preceding gene: 218709826

Following gene: 218709817

Centisome position: 59.94

GC content: 46.87

Gene sequence:

>1293_bases
ATGTTTCAATTTATGACATCTACAAAGATCATCTTTGGTGATGGAGCACTCTCCGCTTCATTGTCTCTCTTTAATCAATA
CGGTTACAGCGTGCTATTGGTGACTGGCAATACATTAGAGCGCACTTCAATAGTCACTGACTATCTCGATGCACAGAGTA
TGCGCTATCAACACATTGCCGTATCCGGTGAACCTAATATCAAAATGGTTGAGGAGGCTGCTATTTCTGCACGTCGATTT
AAGCCAGATATGGTTGTCGCTATGGGGGGCGGTAGCGCCATCGATATGGGCAAAGCGTTAGCCGCGGTTTTACCTAACCA
AGGTAGTTTGTATGACTATGTTGAAGTCGTTGGGCGTAATGTCCCTCTTAAAACCAAACCACTTCCGTTCATTGCGATTC
CAACCACAGCGAGTACAGGTGCAGAAGTCACTAAAAACGCGGTGCTGAAATCAGGGCAAGACCAAGTGAAAATCAGCCTT
AGAAACCCAGACATGCTGGCTGACGTTGCGATTGTTGACCCAACCCTAACTCATGGTACGAATCTGTATTTATCGGGGCG
AGGTGCTATGGACGCATTTACCCATCTAATGGAAGCCTATGTGTGCGGTGAACCGAACCCACTGACTGATATGATCTGTG
AAGAAGGCTTACGTAAGCTGAGTGGTTCTGTCATTCAGGCGTGTATCTACGATGAACCTCAAGCGCGTTCTGATCTCGCG
TTTTCCTCTATGTTAGGGGGAATAGCGATAACTAACGCCAAACTTGGTGCTGCGCATGGTTTGGCTTCTGCGCTAGGTGG
CAAAATCTCGGCCCCTCATAGTGTGATAACTGCACGTTTAGCACCATTTGTGATGTTAGAGAATATAGCTGTTGCGAGAG
AACAGCAGAGATGTGACATCCTAGCGCGTTATCAGCGAATTTCTCAGATAGTGACAGGTAACGTAGAAGCAACAGAGGAA
GAGGCGATTTCTTGGTTGTCAGAAGTGTTGGATACGCTCAAGCTTCCAAGCTTACTGGAATTTGGTGTCTGTGAAGCCCA
ATTTGATGAGGTGTCAGCCGACGCGCTCAAATCAGTGGCGATTAAAGGGAACCCTTTACCGCTGAACCAAGAACGGCTCG
TACGTATTCTGAAGAAGGTTTGCGAGAAGTGCGGTTGTGTCGAAGGGCATCATGAGGGAGCTTTAATGAATCAAATTGAG
CAAGAGCCTACCGCTGAGTCGAGCTTTACCATTATCAATTCTTATGCGTCCGAGAATAGCGTGGTTGAGAAGGGCAATAG
CTGGACTATCTAA

Upstream 100 bases:

>100_bases
CAAATTTCAAATGTAAAATTCAAGACACCAATTTCCAGAAATCAATTTTCTAAAAACCAAGAGTTCGCAGTTTTGTTCGG
TCATAGGCGCAGGAGGCACC

Downstream 100 bases:

>100_bases
CAGTCTTCAATCTGCTCTCGGCATAACCAGACACAGTATAACCAATCACAGCACAAACCCATCACGACATAACCCATTTA
AACAGACAAATAAAAACGGA

Product: alcohol dehydrogenase IV

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFQFMTSTKIIFGDGALSASLSLFNQYGYSVLLVTGNTLERTSIVTDYLDAQSMRYQHIAVSGEPNIKMVEEAAISARRF
KPDMVVAMGGGSAIDMGKALAAVLPNQGSLYDYVEVVGRNVPLKTKPLPFIAIPTTASTGAEVTKNAVLKSGQDQVKISL
RNPDMLADVAIVDPTLTHGTNLYLSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRKLSGSVIQACIYDEPQARSDLA
FSSMLGGIAITNAKLGAAHGLASALGGKISAPHSVITARLAPFVMLENIAVAREQQRCDILARYQRISQIVTGNVEATEE
EAISWLSEVLDTLKLPSLLEFGVCEAQFDEVSADALKSVAIKGNPLPLNQERLVRILKKVCEKCGCVEGHHEGALMNQIE
QEPTAESSFTIINSYASENSVVEKGNSWTI

Sequences:

>Translated_430_residues
MFQFMTSTKIIFGDGALSASLSLFNQYGYSVLLVTGNTLERTSIVTDYLDAQSMRYQHIAVSGEPNIKMVEEAAISARRF
KPDMVVAMGGGSAIDMGKALAAVLPNQGSLYDYVEVVGRNVPLKTKPLPFIAIPTTASTGAEVTKNAVLKSGQDQVKISL
RNPDMLADVAIVDPTLTHGTNLYLSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRKLSGSVIQACIYDEPQARSDLA
FSSMLGGIAITNAKLGAAHGLASALGGKISAPHSVITARLAPFVMLENIAVAREQQRCDILARYQRISQIVTGNVEATEE
EAISWLSEVLDTLKLPSLLEFGVCEAQFDEVSADALKSVAIKGNPLPLNQERLVRILKKVCEKCGCVEGHHEGALMNQIE
QEPTAESSFTIINSYASENSVVEKGNSWTI
>Mature_430_residues
MFQFMTSTKIIFGDGALSASLSLFNQYGYSVLLVTGNTLERTSIVTDYLDAQSMRYQHIAVSGEPNIKMVEEAAISARRF
KPDMVVAMGGGSAIDMGKALAAVLPNQGSLYDYVEVVGRNVPLKTKPLPFIAIPTTASTGAEVTKNAVLKSGQDQVKISL
RNPDMLADVAIVDPTLTHGTNLYLSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRKLSGSVIQACIYDEPQARSDLA
FSSMLGGIAITNAKLGAAHGLASALGGKISAPHSVITARLAPFVMLENIAVAREQQRCDILARYQRISQIVTGNVEATEE
EAISWLSEVLDTLKLPSLLEFGVCEAQFDEVSADALKSVAIKGNPLPLNQERLVRILKKVCEKCGCVEGHHEGALMNQIE
QEPTAESSFTIINSYASENSVVEKGNSWTI

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994951, Length=386, Percent_Identity=30.8290155440415, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789163, Length=376, Percent_Identity=28.7234042553192, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082107, Length=375, Percent_Identity=29.3333333333333, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787493, Length=357, Percent_Identity=29.6918767507003, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789386, Length=355, Percent_Identity=21.9718309859155, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=289, Percent_Identity=26.9896193771626, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321181, Length=381, Percent_Identity=29.9212598425197, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24657991, Length=413, Percent_Identity=24.9394673123487, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 46238; Mature: 46238

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQFMTSTKIIFGDGALSASLSLFNQYGYSVLLVTGNTLERTSIVTDYLDAQSMRYQHIA
CCEEEECCEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEE
VSGEPNIKMVEEAAISARRFKPDMVVAMGGGSAIDMGKALAAVLPNQGSLYDYVEVVGRN
ECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
VPLKTKPLPFIAIPTTASTGAEVTKNAVLKSGQDQVKISLRNPDMLADVAIVDPTLTHGT
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCC
NLYLSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRKLSGSVIQACIYDEPQARSDLA
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FSSMLGGIAITNAKLGAAHGLASALGGKISAPHSVITARLAPFVMLENIAVAREQQRCDI
HHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARYQRISQIVTGNVEATEEEAISWLSEVLDTLKLPSLLEFGVCEAQFDEVSADALKSVA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHE
IKGNPLPLNQERLVRILKKVCEKCGCVEGHHEGALMNQIEQEPTAESSFTIINSYASENS
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCC
VVEKGNSWTI
HHCCCCCCCC
>Mature Secondary Structure
MFQFMTSTKIIFGDGALSASLSLFNQYGYSVLLVTGNTLERTSIVTDYLDAQSMRYQHIA
CCEEEECCEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEE
VSGEPNIKMVEEAAISARRFKPDMVVAMGGGSAIDMGKALAAVLPNQGSLYDYVEVVGRN
ECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
VPLKTKPLPFIAIPTTASTGAEVTKNAVLKSGQDQVKISLRNPDMLADVAIVDPTLTHGT
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCC
NLYLSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRKLSGSVIQACIYDEPQARSDLA
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FSSMLGGIAITNAKLGAAHGLASALGGKISAPHSVITARLAPFVMLENIAVAREQQRCDI
HHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARYQRISQIVTGNVEATEEEAISWLSEVLDTLKLPSLLEFGVCEAQFDEVSADALKSVA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHE
IKGNPLPLNQERLVRILKKVCEKCGCVEGHHEGALMNQIEQEPTAESSFTIINSYASENS
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCC
VVEKGNSWTI
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.005 {cyclohexanol}} 0.004 {

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]