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The map label for this gene is fumB [H]

Identifier: 218709785

GI number: 218709785

Start: 1924427

End: 1926112

Strand: Reverse

Name: fumB [H]

Synonym: VS_1797

Alternate gene names: 218709785

Gene position: 1926112-1924427 (Counterclockwise)

Preceding gene: 218709787

Following gene: 218709784

Centisome position: 58.38

GC content: 46.14

Gene sequence:

>1686_bases
TTGATTAGAACGGTGCAAACTACATGGTTTAGTTTACAGCTATGCAAGCGATTGCTAAAGCAACTAAATACAGCTCAACA
CAATAAAAAGCTAAGCCAACTAATCCGGTTAGACAAAAACAGTCAAACCAAGACCCCACATGGAACCAGAACAATAAAGA
GGCATGCAATGACGGTTATTCGTAAGCAAGATGTGATCAGCAGTGTCGCTGACGCACTTCAGTACATTTCTTATTATCAC
CCTTTAGACTTTGTCCAAGCCCTAGAAAAAGCGTACGAGAAAGAAGAGAGCCAAGCAGCTAAAGATGCTATTGCTCAGAT
TCTTATCAACTCACGTATGTCTGCGGAAGGCCATCGTCCAATTTGTCAGGATACGGGTATTGTTACTTGTTTCGTGAACA
TCGGTATGGATGTTAGGTGGGAAACGGATCAAACAGTACAACAGATGGTTGATGAAGGCGTTCGTCAAGCTTACAACAAT
CCAGATAACCCATTGCGTGCATCTGTCCTAATGGATCCTGCAGGTAAGCGTATTAATACCAAAGACAACACACCAGCGGT
TGTTCACATTAATATGGTTCCTGGCAACAAAGTTGAAATTCAAATCGCAGCAAAAGGCGGCGGTTCTGAGAACAAAACCA
AGATGGTTATGCTGAACCCTTCTGATGATATTGCAGAATGGGTAGAGAAGACGCTACCAACAATGGGTGCGGGCTGGTGT
CCACCGGGCATGCTAGGCATAGGTATTGGCGGTACGGCAGAGAAAGCAGCAGTACTAGCAAAAGAATCTCTGATGGAACA
CATCGATATTCAAGAGCTTATCGACAAAGGCCCAGAGAACGCTGAAGAAGAGCTTCGTTTAGATATCTTCAACCGTGTAA
ATAAACTGGGCATTGGCGCACAAGGTCTTGGCGGTCTAACGACAGTGGTTGACGTGAAAATCAAAACAGCGCCAACGCAC
GCAGCCTCTAAGCCTGTATGCTTGATCCCGAACTGTGCAGCAACGCGTCACGTACACTTCACATTAGATGGCAGCGGCCC
TGCAGAACTAACGCCACCTAAGCTAGAAGAGTGGCCAGATATTACTTGGGAAGCAGGCGCAAATACACGCCGTGTTAACC
TTGATGAAATTACTAAAGAAGACGTTCAAGAGTGGAAGACCGGCGAAACGGTTCTTCTATCAGGAAAGATCTTAACCGGT
CGTGATGCAGCGCATAAGCGTATTCAAGGCATGCTTGAAAGTGGTGAAGGTTTACCAGAAGGCGTCGACCTGAAAGGTAA
GTTCATTTACTACGTTGGCCCTGTTGATGCTGTGGGTGATGAAGCGGTAGGTCCTGCTGGCCCGACAACGTCAACTCGTA
TGGATAAGTTCACCGACATGATGCTAGAAGAAACCGGCATTATGGGCATGATTGGTAAAGCAGAACGTGGCCCTGCGACC
GTTGAATCAATCAAACAACATAAATCGGTTTACCTGATGGCGGTTGGTGGTGCGGCTTATTTAGTCGCGAAAGCCATTAA
AAAAGCACGTGTGGTTGCGTTTGAAGATCTCGGTATGGAAGCGATTTACGAGTTTGAAGTTGAAGATATGCCTGTAACGG
TTGCGGTTGACTCTAACGGCGTGAACGCGCACCAGATCGGTCCTGACACGTGGAAAGTGAAAATTGCTGAAGCTGAAAAA
GCATAA

Upstream 100 bases:

>100_bases
TCGTTGTTATTCATTTGTTTAATTTGTGACAAAGAGTTCGATCACTGCATGGCGTGTGAGAGGAAGCAAGAGTATCATAA
ATTGAAAATAAAATGGGTTC

Downstream 100 bases:

>100_bases
TTTACCGAAGTCAAAAAATCGTAATAGACACTAACTTCAGCGTTTGAATTGCAAACCTTGACAGAAAGTTGAAGACAAAA
GTGCAGCATTATTTGCACTT

Product: fumarate hydratase class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MIRTVQTTWFSLQLCKRLLKQLNTAQHNKKLSQLIRLDKNSQTKTPHGTRTIKRHAMTVIRKQDVISSVADALQYISYYH
PLDFVQALEKAYEKEESQAAKDAIAQILINSRMSAEGHRPICQDTGIVTCFVNIGMDVRWETDQTVQQMVDEGVRQAYNN
PDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTLPTMGAGWC
PPGMLGIGIGGTAEKAAVLAKESLMEHIDIQELIDKGPENAEEELRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKTAPTH
AASKPVCLIPNCAATRHVHFTLDGSGPAELTPPKLEEWPDITWEAGANTRRVNLDEITKEDVQEWKTGETVLLSGKILTG
RDAAHKRIQGMLESGEGLPEGVDLKGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLEETGIMGMIGKAERGPAT
VESIKQHKSVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAVDSNGVNAHQIGPDTWKVKIAEAEK
A

Sequences:

>Translated_561_residues
MIRTVQTTWFSLQLCKRLLKQLNTAQHNKKLSQLIRLDKNSQTKTPHGTRTIKRHAMTVIRKQDVISSVADALQYISYYH
PLDFVQALEKAYEKEESQAAKDAIAQILINSRMSAEGHRPICQDTGIVTCFVNIGMDVRWETDQTVQQMVDEGVRQAYNN
PDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTLPTMGAGWC
PPGMLGIGIGGTAEKAAVLAKESLMEHIDIQELIDKGPENAEEELRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKTAPTH
AASKPVCLIPNCAATRHVHFTLDGSGPAELTPPKLEEWPDITWEAGANTRRVNLDEITKEDVQEWKTGETVLLSGKILTG
RDAAHKRIQGMLESGEGLPEGVDLKGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLEETGIMGMIGKAERGPAT
VESIKQHKSVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAVDSNGVNAHQIGPDTWKVKIAEAEK
A
>Mature_561_residues
MIRTVQTTWFSLQLCKRLLKQLNTAQHNKKLSQLIRLDKNSQTKTPHGTRTIKRHAMTVIRKQDVISSVADALQYISYYH
PLDFVQALEKAYEKEESQAAKDAIAQILINSRMSAEGHRPICQDTGIVTCFVNIGMDVRWETDQTVQQMVDEGVRQAYNN
PDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTLPTMGAGWC
PPGMLGIGIGGTAEKAAVLAKESLMEHIDIQELIDKGPENAEEELRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKTAPTH
AASKPVCLIPNCAATRHVHFTLDGSGPAELTPPKLEEWPDITWEAGANTRRVNLDEITKEDVQEWKTGETVLLSGKILTG
RDAAHKRIQGMLESGEGLPEGVDLKGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLEETGIMGMIGKAERGPAT
VESIKQHKSVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAVDSNGVNAHQIGPDTWKVKIAEAEK
A

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=477, Percent_Identity=30.8176100628931, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787897, Length=470, Percent_Identity=32.7659574468085, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789442, Length=276, Percent_Identity=29.7101449275362, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=33.5294117647059, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 61235; Mature: 61235

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTVQTTWFSLQLCKRLLKQLNTAQHNKKLSQLIRLDKNSQTKTPHGTRTIKRHAMTVI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RKQDVISSVADALQYISYYHPLDFVQALEKAYEKEESQAAKDAIAQILINSRMSAEGHRP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ICQDTGIVTCFVNIGMDVRWETDQTVQQMVDEGVRQAYNNPDNPLRASVLMDPAGKRINT
CCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
KDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTLPTMGAGWC
CCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCC
PPGMLGIGIGGTAEKAAVLAKESLMEHIDIQELIDKGPENAEEELRLDIFNRVNKLGIGA
CCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
QGLGGLTTVVDVKIKTAPTHAASKPVCLIPNCAATRHVHFTLDGSGPAELTPPKLEEWPD
CCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHCCC
ITWEAGANTRRVNLDEITKEDVQEWKTGETVLLSGKILTGRDAAHKRIQGMLESGEGLPE
CEEECCCCCEECCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHHHHHCCCCCCC
GVDLKGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLEETGIMGMIGKAERGPAT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHH
VESIKQHKSVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAVDSNG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCCEEEEECCCC
VNAHQIGPDTWKVKIAEAEKA
CCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MIRTVQTTWFSLQLCKRLLKQLNTAQHNKKLSQLIRLDKNSQTKTPHGTRTIKRHAMTVI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RKQDVISSVADALQYISYYHPLDFVQALEKAYEKEESQAAKDAIAQILINSRMSAEGHRP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ICQDTGIVTCFVNIGMDVRWETDQTVQQMVDEGVRQAYNNPDNPLRASVLMDPAGKRINT
CCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
KDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTLPTMGAGWC
CCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCC
PPGMLGIGIGGTAEKAAVLAKESLMEHIDIQELIDKGPENAEEELRLDIFNRVNKLGIGA
CCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
QGLGGLTTVVDVKIKTAPTHAASKPVCLIPNCAATRHVHFTLDGSGPAELTPPKLEEWPD
CCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHCCC
ITWEAGANTRRVNLDEITKEDVQEWKTGETVLLSGKILTGRDAAHKRIQGMLESGEGLPE
CEEECCCCCEECCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHHHHHCCCCCCC
GVDLKGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLEETGIMGMIGKAERGPAT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHH
VESIKQHKSVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAVDSNG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCCEEEEECCCC
VNAHQIGPDTWKVKIAEAEKA
CCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]