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The map label for this gene is dosC [H]

Identifier: 218709773

GI number: 218709773

Start: 1910296

End: 1911834

Strand: Reverse

Name: dosC [H]

Synonym: VS_1785

Alternate gene names: 218709773

Gene position: 1911834-1910296 (Counterclockwise)

Preceding gene: 218709778

Following gene: 218709772

Centisome position: 57.95

GC content: 41.78

Gene sequence:

>1539_bases
ATGCCCCAAACTACTGACCCACTAACAGGGTTGAAGAGACAAACTCTCCCGTTGGTCGTATTTTCGGTCAGTTTTTTGAT
TACTGTTTTGTGCTTTGTATTAGTCATGCTTAATCAAAAGCGTGCGCTGAATATGAATTTGGATAGTTTTGCTACTCACC
AGACATTGAGCTTGGAAGCCTTTATAGATAATGATGTGGCATATATTGGGTCTGGTGCTAATTTTTTCTACGCAAATGAC
CCTGAAAATTGGGATAAGTTTGATCGCTTTGCTAAGCAAACCATAGATGATTCAAAGAGTTTAATTGCCTTGCAGTGGAT
GCAAAAAGTGCCCGCAGACGAGATAGCTGAGCACACTGCGAAAATGAAAGAAAAGTACCCATTCTTTAAGCTGTATACCA
TTCCTAAGAACCAAGCAAAAACCTATGGCTACATTTTGGATGGTCAGCCAGCTTTCGTTGCTAGCGATATTTTCCCTAAT
ACAGAACCAAACAACAAAGTTCTTGGCTTTTACTCTTCTAGAGAGCGATTTGATCTCGTTCTAGATAATATCAAACAGAC
ACGTAAGGCGAATATTTCAGACAAAGTTCGCTTGATACAAGATGGGCTAGATAAAGACACAAAAAAGAGTGGCATGTTGG
TGTATCACCCTGTCTTTGAGGGAGAGAGTGACAACTTACTCGGCGTTGTAATTGGTGTGGTTCGCACCACATACTATTTT
GAGAACTTGCTGGCTTCGACGGTTGGGGATATGAATGTTTATGTCCGCGTTAGTGATGTCGGTTTTGAAGCCGAAGATGC
ACCTGTCTTGTTCGAAACGAAAGGTTATGAGGGTGTGACTGGACAACATATCACCAAGAAAATCACACTCACTAACCGTG
AATGGGTTGTCGACTTCAAGATAGACTCATGCATTTCGTTTAATGGTTACCTGGTGTTGGCCGGGATTGCCCTCGTGGGC
ACGACTATTTCACTGTTGCTTTCTTATATCGTTAAACTGCAAGTCCAAGAAAAAGTACGCCTGTATAAAATGATTGATGA
AAGAACGGAAGAACTCCGTTTTCTTGCCAATCATGACAGCCTCACAGAGATTTATAATCGACGAGCCTTTAATAAGGTGC
TCGATAAAGCGATACAAGGTGACAAGCCGTTTAGCTTGATTGGATTTGATGTCGATAAATTTAAAGGGATTAATGATCAA
TTTGGCCACCCGGCTGGTGATGCCTTACTGGTTCATGTAATTCAGTTGATTTCAGGCAGTTTGAAAGAGGGCGATCATGT
CTTCCGTATGGGAGGAGATGAGTTTGGTATCATTTCGAGCGTCATTGAACACGACGCGCTGGCACGTTATTTAGAATGTA
TTTTAGATACGGTGAGCGAGTCTCACTGTGAGCATAAAGGTCAACTATTGAGTTGTACTCTAAGTATCGGTGCTACCATC
CGCAGTAAAGAAGACATCGAAGAGCTCCTTCAAAAAACCGATAGTATGCTTTACCTAAGTAAGTCAAATGGCCGAAATCG
CGTTACAATTGCAGGCTAA

Upstream 100 bases:

>100_bases
AAAATGCCCGTTTAATGTGACATTTAGGACTAAACATGAATGGAGCATTATTTCCGTCCGTTATATACTTTTCATTCAAT
AGAGCCTATTTGGAACCCGC

Downstream 100 bases:

>100_bases
ATGAGCACTTGATGCAACTAGGGTCGAGTTCTGTATTGGGCTCATGTACAATTTGACAAAATTTTTGACGAGAGAATTAG
GCGACGTATGAATCACAATC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MPQTTDPLTGLKRQTLPLVVFSVSFLITVLCFVLVMLNQKRALNMNLDSFATHQTLSLEAFIDNDVAYIGSGANFFYAND
PENWDKFDRFAKQTIDDSKSLIALQWMQKVPADEIAEHTAKMKEKYPFFKLYTIPKNQAKTYGYILDGQPAFVASDIFPN
TEPNNKVLGFYSSRERFDLVLDNIKQTRKANISDKVRLIQDGLDKDTKKSGMLVYHPVFEGESDNLLGVVIGVVRTTYYF
ENLLASTVGDMNVYVRVSDVGFEAEDAPVLFETKGYEGVTGQHITKKITLTNREWVVDFKIDSCISFNGYLVLAGIALVG
TTISLLLSYIVKLQVQEKVRLYKMIDERTEELRFLANHDSLTEIYNRRAFNKVLDKAIQGDKPFSLIGFDVDKFKGINDQ
FGHPAGDALLVHVIQLISGSLKEGDHVFRMGGDEFGIISSVIEHDALARYLECILDTVSESHCEHKGQLLSCTLSIGATI
RSKEDIEELLQKTDSMLYLSKSNGRNRVTIAG

Sequences:

>Translated_512_residues
MPQTTDPLTGLKRQTLPLVVFSVSFLITVLCFVLVMLNQKRALNMNLDSFATHQTLSLEAFIDNDVAYIGSGANFFYAND
PENWDKFDRFAKQTIDDSKSLIALQWMQKVPADEIAEHTAKMKEKYPFFKLYTIPKNQAKTYGYILDGQPAFVASDIFPN
TEPNNKVLGFYSSRERFDLVLDNIKQTRKANISDKVRLIQDGLDKDTKKSGMLVYHPVFEGESDNLLGVVIGVVRTTYYF
ENLLASTVGDMNVYVRVSDVGFEAEDAPVLFETKGYEGVTGQHITKKITLTNREWVVDFKIDSCISFNGYLVLAGIALVG
TTISLLLSYIVKLQVQEKVRLYKMIDERTEELRFLANHDSLTEIYNRRAFNKVLDKAIQGDKPFSLIGFDVDKFKGINDQ
FGHPAGDALLVHVIQLISGSLKEGDHVFRMGGDEFGIISSVIEHDALARYLECILDTVSESHCEHKGQLLSCTLSIGATI
RSKEDIEELLQKTDSMLYLSKSNGRNRVTIAG
>Mature_511_residues
PQTTDPLTGLKRQTLPLVVFSVSFLITVLCFVLVMLNQKRALNMNLDSFATHQTLSLEAFIDNDVAYIGSGANFFYANDP
ENWDKFDRFAKQTIDDSKSLIALQWMQKVPADEIAEHTAKMKEKYPFFKLYTIPKNQAKTYGYILDGQPAFVASDIFPNT
EPNNKVLGFYSSRERFDLVLDNIKQTRKANISDKVRLIQDGLDKDTKKSGMLVYHPVFEGESDNLLGVVIGVVRTTYYFE
NLLASTVGDMNVYVRVSDVGFEAEDAPVLFETKGYEGVTGQHITKKITLTNREWVVDFKIDSCISFNGYLVLAGIALVGT
TISLLLSYIVKLQVQEKVRLYKMIDERTEELRFLANHDSLTEIYNRRAFNKVLDKAIQGDKPFSLIGFDVDKFKGINDQF
GHPAGDALLVHVIQLISGSLKEGDHVFRMGGDEFGIISSVIEHDALARYLECILDTVSESHCEHKGQLLSCTLSIGATIR
SKEDIEELLQKTDSMLYLSKSNGRNRVTIAG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3614

COG function: function code T; Predicted periplasmic ligand-binding sensor domain

Gene ontology:

Cell location: Integral membrane protein (Potential) [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=196, Percent_Identity=36.2244897959184, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=35.4838709677419, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=32.4324324324324, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=35.5029585798817, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=30.8641975308642, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=213, Percent_Identity=26.2910798122066, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=30.5389221556886, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=226, Percent_Identity=27.8761061946903, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=154, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=30.5389221556886, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=26.875, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=29.2207792207792, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57609; Mature: 57477

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50839 CHASE ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTTDPLTGLKRQTLPLVVFSVSFLITVLCFVLVMLNQKRALNMNLDSFATHQTLSLEA
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCEEEEEE
FIDNDVAYIGSGANFFYANDPENWDKFDRFAKQTIDDSKSLIALQWMQKVPADEIAEHTA
EECCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHH
KMKEKYPFFKLYTIPKNQAKTYGYILDGQPAFVASDIFPNTEPNNKVLGFYSSRERFDLV
HHHHCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHH
LDNIKQTRKANISDKVRLIQDGLDKDTKKSGMLVYHPVFEGESDNLLGVVIGVVRTTYYF
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ENLLASTVGDMNVYVRVSDVGFEAEDAPVLFETKGYEGVTGQHITKKITLTNREWVVDFK
HHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCEEEEEEE
IDSCISFNGYLVLAGIALVGTTISLLLSYIVKLQVQEKVRLYKMIDERTEELRFLANHDS
ECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LTEIYNRRAFNKVLDKAIQGDKPFSLIGFDVDKFKGINDQFGHPAGDALLVHVIQLISGS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
LKEGDHVFRMGGDEFGIISSVIEHDALARYLECILDTVSESHCEHKGQLLSCTLSIGATI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RSKEDIEELLQKTDSMLYLSKSNGRNRVTIAG
CCHHHHHHHHHHCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
PQTTDPLTGLKRQTLPLVVFSVSFLITVLCFVLVMLNQKRALNMNLDSFATHQTLSLEA
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCEEEEEE
FIDNDVAYIGSGANFFYANDPENWDKFDRFAKQTIDDSKSLIALQWMQKVPADEIAEHTA
EECCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHH
KMKEKYPFFKLYTIPKNQAKTYGYILDGQPAFVASDIFPNTEPNNKVLGFYSSRERFDLV
HHHHCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHH
LDNIKQTRKANISDKVRLIQDGLDKDTKKSGMLVYHPVFEGESDNLLGVVIGVVRTTYYF
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ENLLASTVGDMNVYVRVSDVGFEAEDAPVLFETKGYEGVTGQHITKKITLTNREWVVDFK
HHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCEEEEEEE
IDSCISFNGYLVLAGIALVGTTISLLLSYIVKLQVQEKVRLYKMIDERTEELRFLANHDS
ECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LTEIYNRRAFNKVLDKAIQGDKPFSLIGFDVDKFKGINDQFGHPAGDALLVHVIQLISGS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
LKEGDHVFRMGGDEFGIISSVIEHDALARYLECILDTVSESHCEHKGQLLSCTLSIGATI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RSKEDIEELLQKTDSMLYLSKSNGRNRVTIAG
CCHHHHHHHHHHCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]