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The map label for this gene is hupB [H]

Identifier: 218709724

GI number: 218709724

Start: 1863117

End: 1863392

Strand: Direct

Name: hupB [H]

Synonym: VS_1735

Alternate gene names: 218709724

Gene position: 1863117-1863392 (Clockwise)

Preceding gene: 218709723

Following gene: 218709725

Centisome position: 56.47

GC content: 44.2

Gene sequence:

>276_bases
ATGAACAAATCTCAATTAGTTGAACACATCGCAACTTCTGCTGACATCTCAAAAGAGCAAGCAGGCACAGCACTCAATGC
ACTCGTTGAAGGCATCTCGACTACGCTTGCTAATGGCGACGATGTATCTATCCTTGGATTTGGTAGCTTTAAAGTGAATA
GCCGCGCTGCTCGCACTGGTCGCAATCCAAGAACTGGAGAAGAGATTCAAATCTCAGCGTCAAAAACACCAGCATTTAAA
GCAGGTAAAGCACTAAAAGAGACATGCAACCTGTAA

Upstream 100 bases:

>100_bases
TCAATCCTAGCAATGACATAACTTCAGTTCATATTGGTCATATTTAACCAATAGTTTGCAAAAACCAAACGCCAGACGAA
CTCGACAAGGAATTTAAAAC

Downstream 100 bases:

>100_bases
GATAGAAAATACAAGGAAGGTTACCGATGACAACTAAGGAAAAAACAAAAACGAGCCGCAGTAAAAAAGCCGGCAAGTCA
ACCAAAGCACAAGCTGAAGG

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MNKSQLVEHIATSADISKEQAGTALNALVEGISTTLANGDDVSILGFGSFKVNSRAARTGRNPRTGEEIQISASKTPAFK
AGKALKETCNL

Sequences:

>Translated_91_residues
MNKSQLVEHIATSADISKEQAGTALNALVEGISTTLANGDDVSILGFGSFKVNSRAARTGRNPRTGEEIQISASKTPAFK
AGKALKETCNL
>Mature_91_residues
MNKSQLVEHIATSADISKEQAGTALNALVEGISTTLANGDDVSILGFGSFKVNSRAARTGRNPRTGEEIQISASKTPAFK
AGKALKETCNL

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=58.8888888888889, Blast_Score=117, Evalue=1e-28,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=53.9325842696629, Blast_Score=102, Evalue=5e-24,
Organism=Escherichia coli, GI1787141, Length=92, Percent_Identity=40.2173913043478, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1788005, Length=86, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9506; Mature: 9506

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSQLVEHIATSADISKEQAGTALNALVEGISTTLANGDDVSILGFGSFKVNSRAARTG
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCC
RNPRTGEEIQISASKTPAFKAGKALKETCNL
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNKSQLVEHIATSADISKEQAGTALNALVEGISTTLANGDDVSILGFGSFKVNSRAARTG
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCC
RNPRTGEEIQISASKTPAFKAGKALKETCNL
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]