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The map label for this gene is sph [H]

Identifier: 218709697

GI number: 218709697

Start: 1834580

End: 1835914

Strand: Direct

Name: sph [H]

Synonym: VS_1708

Alternate gene names: 218709697

Gene position: 1834580-1835914 (Clockwise)

Preceding gene: 218709695

Following gene: 218709698

Centisome position: 55.61

GC content: 48.09

Gene sequence:

>1335_bases
ATGAAAACTCAATGGACTTGGTTAGCTGCACTTGTGGCATCCACATCAATCGCTTCGACCTCTGCGATCGCAGATACCGA
CGTGTATCTCACCAATAACACCAATCAAGTGATGACCATTCAAGCCAACCACACTGGGACCGACTTGCTTCAACTCGGTG
ATGAGTGGCAACAGCATGTTGAACAGATTGGTCCTTGGGAGACGAAGAAGCTGATCAGTTTTAACCGCTGGACTGGTGTG
AAATCTGGGAAGACCTATGAGTTTGACACTGTGGTATCGAACGCTGTGGGTGAGAGTGTCACGCTCAACCAAACCATGAA
GGGTCATTGGTACAACTCGACACTGCAACATGGTTTAAGCGCCGCAGACGTCAACCTAACGTTGCATGACGACCGTAATA
TTCATCGCAGCACAACCGATGCGTTCGGGGTCAATGCAGAGCTAGCACTTAAAGCCGATTCTACGGCGCGCTACGATGAT
ATTTATTACACCATCACTCCACCAAAAGTGGATGAGCAACCAGAGCCAGATGCCAATACACTCAAAGTCATAACTTATAA
CATCTGGGCACTGCCTGCCATCGCTTCGCATATTGGTGACCGCTATGACCTGATCCCTCAATACGTAAAGGGCTATGACG
TGTTGGCGCTACAAGAGGTGTTTGCTAACGGCAGAGATGAATTCTTGCGTGAACTTGCAAAAGAGTATCCTTATCAAACC
AAAATGCTCGATAAAGATGGGATTAACATCTATGACGGCGGTGTGATAATTGTGAGCCGCTACCCTATTGTCAACGAAGC
GCAATACGTGTTCCCTGATTGTACAGGCACAGATTGTTTCGCAGATAAAGGCGTGAATTACGCTGAAGTCATCAAAAACG
GTCAGGCTTACCACGTGTTTGGCACTCACACCGCATCATTCGATACGGACACCGCTCGTGATTACCGACAACGTCAATTC
AAGCAGATGCGTGCACTGGCGCAGTCTCTGAATATCCCAGCATCAGAAACTGTGGTTTACAGCGGTGACTTCAACGTGAA
CAAGCTGAAGTTCCCAGACGACTACCAACAGATGTTCGCTAACCTGCAGGCTGATAAACCAACATACTCTGGTTACACCG
CTTCTACCTTTGACCCACGCATCAACAACTTTGCGGGCGAACCTATGTCGGGTGGCGAGAACGTTGAGTATCTAGATTAT
GTGGTGGTGAGTTCAGAGTATGCAGCTAAGGCTCATAACGACAATCGCGTGGATGTCCCAAGATCAACAAGCAGTGAGTT
ATGGAAGCACTACAACCTTTCAGACCACTTCCCTGTTAGCGCCGTGATCAAGTAA

Upstream 100 bases:

>100_bases
TATATCTCATATTTGAATAAATGCCATAGAGTTATAACTCCAATGTCATAACTCTTGACTACGTTATTCTAGACATCAAT
CAATAAGGATAACGTAAACG

Downstream 100 bases:

>100_bases
CTCGGTGAACCAATAATGCATCGAGTTATATTAATCCTAATGCTCGTCTTAACGGCGAGCATTGTTGGCTACTCATGGTC
ATCAAAATCGATTGAAACAC

Product: putative phospholipase C precursor

Products: NA

Alternate protein names: SMase; Cereolysin B; SMPLC; Sphingomyelin phosphodiesterase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKTQWTWLAALVASTSIASTSAIADTDVYLTNNTNQVMTIQANHTGTDLLQLGDEWQQHVEQIGPWETKKLISFNRWTGV
KSGKTYEFDTVVSNAVGESVTLNQTMKGHWYNSTLQHGLSAADVNLTLHDDRNIHRSTTDAFGVNAELALKADSTARYDD
IYYTITPPKVDEQPEPDANTLKVITYNIWALPAIASHIGDRYDLIPQYVKGYDVLALQEVFANGRDEFLRELAKEYPYQT
KMLDKDGINIYDGGVIIVSRYPIVNEAQYVFPDCTGTDCFADKGVNYAEVIKNGQAYHVFGTHTASFDTDTARDYRQRQF
KQMRALAQSLNIPASETVVYSGDFNVNKLKFPDDYQQMFANLQADKPTYSGYTASTFDPRINNFAGEPMSGGENVEYLDY
VVVSSEYAAKAHNDNRVDVPRSTSSELWKHYNLSDHFPVSAVIK

Sequences:

>Translated_444_residues
MKTQWTWLAALVASTSIASTSAIADTDVYLTNNTNQVMTIQANHTGTDLLQLGDEWQQHVEQIGPWETKKLISFNRWTGV
KSGKTYEFDTVVSNAVGESVTLNQTMKGHWYNSTLQHGLSAADVNLTLHDDRNIHRSTTDAFGVNAELALKADSTARYDD
IYYTITPPKVDEQPEPDANTLKVITYNIWALPAIASHIGDRYDLIPQYVKGYDVLALQEVFANGRDEFLRELAKEYPYQT
KMLDKDGINIYDGGVIIVSRYPIVNEAQYVFPDCTGTDCFADKGVNYAEVIKNGQAYHVFGTHTASFDTDTARDYRQRQF
KQMRALAQSLNIPASETVVYSGDFNVNKLKFPDDYQQMFANLQADKPTYSGYTASTFDPRINNFAGEPMSGGENVEYLDY
VVVSSEYAAKAHNDNRVDVPRSTSSELWKHYNLSDHFPVSAVIK
>Mature_444_residues
MKTQWTWLAALVASTSIASTSAIADTDVYLTNNTNQVMTIQANHTGTDLLQLGDEWQQHVEQIGPWETKKLISFNRWTGV
KSGKTYEFDTVVSNAVGESVTLNQTMKGHWYNSTLQHGLSAADVNLTLHDDRNIHRSTTDAFGVNAELALKADSTARYDD
IYYTITPPKVDEQPEPDANTLKVITYNIWALPAIASHIGDRYDLIPQYVKGYDVLALQEVFANGRDEFLRELAKEYPYQT
KMLDKDGINIYDGGVIIVSRYPIVNEAQYVFPDCTGTDCFADKGVNYAEVIKNGQAYHVFGTHTASFDTDTARDYRQRQF
KQMRALAQSLNIPASETVVYSGDFNVNKLKFPDDYQQMFANLQADKPTYSGYTASTFDPRINNFAGEPMSGGENVEYLDY
VVVSSEYAAKAHNDNRVDVPRSTSSELWKHYNLSDHFPVSAVIK

Specific function: Required, with sphingomyelinase, to effect target cell lysis (hemolysis) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005135
- InterPro:   IPR017766 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.4.12 [H]

Molecular weight: Translated: 49891; Mature: 49891

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQWTWLAALVASTSIASTSAIADTDVYLTNNTNQVMTIQANHTGTDLLQLGDEWQQHV
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHH
EQIGPWETKKLISFNRWTGVKSGKTYEFDTVVSNAVGESVTLNQTMKGHWYNSTLQHGLS
HHCCCCCHHHHEEECCCCCCCCCCCEEHHHHHHHHCCCCEEECCCCCCCEECHHHHCCCC
AADVNLTLHDDRNIHRSTTDAFGVNAELALKADSTARYDDIYYTITPPKVDEQPEPDANT
EEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCE
LKVITYNIWALPAIASHIGDRYDLIPQYVKGYDVLALQEVFANGRDEFLRELAKEYPYQT
EEEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCEE
KMLDKDGINIYDGGVIIVSRYPIVNEAQYVFPDCTGTDCFADKGVNYAEVIKNGQAYHVF
EEECCCCCEEECCCEEEEECCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHCCCCEEEEE
GTHTASFDTDTARDYRQRQFKQMRALAQSLNIPASETVVYSGDFNVNKLKFPDDYQQMFA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHH
NLQADKPTYSGYTASTFDPRINNFAGEPMSGGENVEYLDYVVVSSEYAAKAHNDNRVDVP
HCCCCCCCCCCEECCCCCCCHHCCCCCCCCCCCCCEEEEEEEEECCHHHHCCCCCCCCCC
RSTSSELWKHYNLSDHFPVSAVIK
CCCHHHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MKTQWTWLAALVASTSIASTSAIADTDVYLTNNTNQVMTIQANHTGTDLLQLGDEWQQHV
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHH
EQIGPWETKKLISFNRWTGVKSGKTYEFDTVVSNAVGESVTLNQTMKGHWYNSTLQHGLS
HHCCCCCHHHHEEECCCCCCCCCCCEEHHHHHHHHCCCCEEECCCCCCCEECHHHHCCCC
AADVNLTLHDDRNIHRSTTDAFGVNAELALKADSTARYDDIYYTITPPKVDEQPEPDANT
EEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCE
LKVITYNIWALPAIASHIGDRYDLIPQYVKGYDVLALQEVFANGRDEFLRELAKEYPYQT
EEEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCEE
KMLDKDGINIYDGGVIIVSRYPIVNEAQYVFPDCTGTDCFADKGVNYAEVIKNGQAYHVF
EEECCCCCEEECCCEEEEECCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHCCCCEEEEE
GTHTASFDTDTARDYRQRQFKQMRALAQSLNIPASETVVYSGDFNVNKLKFPDDYQQMFA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHH
NLQADKPTYSGYTASTFDPRINNFAGEPMSGGENVEYLDYVVVSSEYAAKAHNDNRVDVP
HCCCCCCCCCCEECCCCCCCHHCCCCCCCCCCCCCEEEEEEEEECCHHHHCCCCCCCCCC
RSTSSELWKHYNLSDHFPVSAVIK
CCCHHHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2848222; 8387306; 3137122 [H]